Journal articles: 'Bond among walls. eng' – Grafiati (2024)

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Existing masonry and reinforced concrete structures are characterized by a wide use of structural and non-structural masonry members such as structural walls, infill walls, arches, vaults etc. All these members are characterized by high vulnerability when subjected to seismic events, since unreinforced masonry has a negligible tensile strength. The use of fiber reinforced polymers (FRP) composites has become a common practice and it represents a light-weight, easy, fast, and non-invasive solution for rehabilitation of existing masonry structures. Fabric reinforced cementitious matrix (FRCM) are relatively newly developed composite materials, representing a valid alternative to FRP in strengthening and retrofitting of existing reinforced concrete and masonry structures. Despite of the numerous advantages guaranteed by the inorganic matrix, the bond-behavior between the fibers and the embedding matrix is still under investigation. Different set-ups have been proposed in the literature to study the bond behavior of FRCM composites. Among them, single-and double-lap shear tests are the most commonly used. In this paper, the bond behavior of a polyparaphenylene benzobisoxazole (PBO) FRCM composite applied to masonry elements is studied using a bending and a single-lap shear test set-up. The bond capacities obtained by the two set-ups are analyzed and discussed.

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Autoclaved aerated concrete (AAC) blocks are commonly used for masonry walls. In order to understand the strength of AAC masonry, it is essential to assess the tensile and shear bond strengths of the AAC block-mortar interface for various mortar combinations. This research investigates the bond strength of AAC block mortar interface made up of a) polymer modified mortar (PMM) and b) ordinary cement sand mortar of 1:4 or 1:6 ratio with thickness of 10mm, 15mm or 20mm. A thin cement slurry coating was applied on the block surface before placing the cement sand mortar in the masonry. For all types of interface, shear bond strength of masonry was studied using a triplet test, while the tensile bond strength was determined through a cross-couplet test. Among the cement sand mortar used in this study, cement sand mortar of ratio 1:4 and thickness 15mm showed the maximum shear strength of 0.13MPa with the failure of blocks as the predominant failure while the PMM had shear bond strength of 0.12MPa with the failure of blocks as the predominant failure type. However, in case of the tensile bond strength testing, PMM showed the tensile bond strength of 0.19MPa, which was highest among all the test specimens used in this study. Considering both the tensile and shear bond strengths of the AAC masonry and based on the observed failure pattern, among all the combinations used in the experiment, either PMM or cement-sand mortar of ratio 1:4 and thickness of 15mm can be chosen for the AAC masonry.

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Hyperhom*ocysteinemia (HHc) is a new factor being considered at the moment that can cause damage to vessel walls. Its occurrence depends on genetic peculiarities of a body. Our research goal was to estimate frequency of genetic polymorphisms (SNP) in folate cycle genes among people living in Perm region and its influence on hom*ocysteine (Hc) concentration in blood serum. We examined 189 women (32.2±5.25). Hc concentration in blood serum was determined with immune chemiluminescent procedure. We examined frequency of SNP in folate cycle genes with pyrosequencing. hom*ozygote state as per minor alleles in methylene tetrahydrofolate reductase (MTHFR) gene (rs 1801133 и rs 1801131) and MTR gene (rs 1805087) was registered 7.5, 5.4, and 13.75 times less frequently than hom*ozygote state as per neutral alleles. Heterozygote state prevailed for genes of methionine synthase reductase and folate transport protein among examined SNP. hom*ozygotes as per minor allele SNP in MTHFR gene (Ala222Val; rs 1801133) had higher Hc concentration in blood serum that amounted to 8.476 ± 3.193 mmol/L and was 1.276 times higher than the same parameter in hom*ozygotes as per neutral allele (р=0.0036). We didn’t establish any influence on Hc contents in blood serum for the remaining 4 SNP in folate cycle genes (р> 0.1). Examined SNP in MTHFR and MTR genes tended to have neutral alleles more frequently than minor ones. SNP in genes of other examined proteins belonging to folate cycle didn’t have any differences in frequency of examined alleles. We didn’t detect a combination of hom*ozygote state as per two SNP in MTHFR gene or hom*ozygote state as per one SNP and heterozygote state as per another one in a genome. Only SNP in MTHFR gene (Ala222Val, rs 1801133) authentically causes increase in hom*ocysteine concentration out of all the examined SNP in genes of folate cycle enzymes and proteins

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Hyperhom*ocysteinemia (HHc) is a new factor being considered at the moment that can cause damage to vessel walls. Its occurrence depends on genetic peculiarities of a body. Our research goal was to estimate frequency of genetic polymorphisms (SNP) in folate cycle genes among people living in Perm region and its influence on hom*ocysteine (Hc) concentration in blood serum. We examined 189 women (32.2±5.25). Hc concentration in blood serum was determined with immune chemiluminescent procedure. We examined frequency of SNP in folate cycle genes with pyrosequencing. hom*ozygote state as per minor alleles in methylene tetrahydrofolate reductase (MTHFR) gene (rs 1801133 и rs 1801131) and MTR gene (rs 1805087) was registered 7.5, 5.4, and 13.75 times less frequently than hom*ozygote state as per neutral alleles. Heterozygote state prevailed for genes of methionine synthase reductase and folate transport protein among examined SNP. hom*ozygotes as per minor allele SNP in MTHFR gene (Ala222Val; rs 1801133) had higher Hc concentration in blood serum that amounted to 8.476 ± 3.193 mmol/L and was 1.276 times higher than the same parameter in hom*ozygotes as per neutral allele (р=0.0036). We didn’t establish any influence on Hc contents in blood serum for the remaining 4 SNP in folate cycle genes (р> 0.1). Examined SNP in MTHFR and MTR genes tended to have neutral alleles more frequently than minor ones. SNP in genes of other examined proteins belonging to folate cycle didn’t have any differences in frequency of examined alleles. We didn’t detect a combination of hom*ozygote state as per two SNP in MTHFR gene or hom*ozygote state as per one SNP and heterozygote state as per another one in a genome. Only SNP in MTHFR gene (Ala222Val, rs 1801133) authentically causes increase in hom*ocysteine concentration out of all the examined SNP in genes of folate cycle enzymes and proteins

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The cleaning of cavity walls aims to improve adhesive restorative procedures and longevity of restorations. This study has compared the effect of three cleaning agents - sodium bicarbonate jet (Profi II, Dabi Atlante, São Paulo, Brazil); pumice paste plus a biologic detergent (Tergestesim, Probem, São Paulo, Brazil); air water spray - on the bond strength between dentin and two different adhesive systems: Clearfil SE Bond (Kuraray, Kioto, Japan) and Scotchbond Multi-Purpose Plus (3M-ESPE, São Paulo, Brazil). Six groups (n:10) of dental fragments obtained from young adult extracted teeth were prepared, and each one received one of the listed surface cleaning techniques. After the adhesive application, a cone-shaped test body was built with AP-X (Kuraray, Kioto, Japan) or Z100 (3M-ESPE, São Paulo, Brazil) composite resins, using a Teflon matrix. The specimens were tested for tensile bond strength after one-week storage in distilled water at 37°C. Two pairs of fractured specimens of each group were randomly chosen and processed for scanning electron microscopy (SEM) analysis. ANOVA test of the bond strength values showed no statistical differences among the cleaning agents and neither between their interactions with the bonding systems. Upon SEM analysis, most surfaces showed mixed fractures of adhesive and cohesive failures in bonding resin to dentin. Based on statistical and SEM analysis, it was concluded that the cleaning agents studied did not interfere with the bond strength of the adhesive systems used to dentin.

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Abstract Osteoporosis characterized by low bone mineral density (BMD) as assessed by dual-energy X-ray absorptiometry (DXA) is common among end-stage renal disease (ESRD) patients and associates with high fracture incidence and high all-cause mortality. This is because chronic kidney disease-mineral bone disorders (CKD-MBDs) promote not only bone disease (osteoporosis and renal dystrophy) but also vascular calcification and cardiovascular disease. The disturbed bone metabolism in ESRD leads to ‘loss of cortical bone’ with increased cortical porosity and thinning of cortical bone rather than to loss of trabecular bone. Low BMD, especially at cortical-rich bone sites, is closely linked to CKD-MBD, vascular calcification and poor cardiovascular outcomes. These effects appear to be largely mediated by shared mechanistic pathways via the ‘bone–vascular axis’ through which impaired bone status associates with changes in the vascular wall. Thus, bone is more than just the scaffolding that holds the body together and protects organs from external forces but is—in addition to its physical supportive function—also an active endocrine organ that interacts with the vasculature by paracrine and endocrine factors through pathways including Wnt signalling, osteoprotegerin (OPG)/receptor activator of nuclear factor-κB (RANK)/RANK ligand system and the Galectin-3/receptor of advanced glycation end products axis. The insight that osteogenesis and vascular calcification share many similarities—and the knowledge that vascular calcification is a cell-mediated active rather than a passive mineralization process—suggest that low BMD and vascular calcification (‘vascular ossification’) to a large extent represent two sides of the same coin. Here, we briefly review changes of BMD in ESRD as observed using different DXA methods (central and whole-body DXA) at different bone sites for BMD measurements, and summarize recent knowledge regarding the relationships between ‘low BMD’ and ‘fracture incidence, vascular calcification and increased mortality’ in ESRD patients, as well as potential ‘molecular mechanisms’ underlying these associations.

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ABSTRACT Background Smear layer is a negative factor which prevents adhesion of the filling material to the dentinal walls. Recent advances in dental research have incorporated lasers as a potential adjunct in root canal treatment by removing the smear layer before filling the root canal system, enhancing the adhesion of sealers to dentin and improving the sealing ability. Aim To evaluate the microtensile bond strength of AH-Plus resin-based sealer to dentin after treatment with 980 nm diode and 1,064 nm neodymium-doped:yttrium aluminum garnet (Nd:YAG) laser in vitro. Materials and methods Thirty specimens prepared for three groups namely group I (control), group II (980 nm diode–lased specimens) and group III (Nd:YAG–lased specimens). One tooth from each group was observed under scanning electron microscope for evaluation of intracanal root dentin morphology. Remaining specimens were used for making microsections by hard tissue microtome. Specimens for groups II and III were lased with 980 nm diode and 1,064 nm Nd:YAG laser. AH Plus sealer was applied onto specimens and mounted onto Instron universal testing machine for microtensile bond strength testing. Results were subjected to statistical analysis using one-way analysis of variance (ANOVA) and Tukey's test. Results Group III Nd:YAG had maximum mean microtensile bond strength values (11.558 ± 0.869), followed by group II diode (9.073 ± 0.468) and group I control (6.05 ± 0.036). Statistically significant differences were seen among all the groups. SEM analysis shows removal of smear layer in both groups II and III. Conclusion Both Nd:YAG and diode laser were more effective than control group in improving the microtensile bond strength of AH Plus sealer to dentin. Clinical significance Lasers have the potential to increase the adhesiveness of root canal sealer to dentin surface, thereby improving the quality of root canal obturation. How to cite this article Maneesha D, Anil KG, Sindhu R, Surendranath G, Deepak S. An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser. J Contemp Dent Pract 2013;14(2): 183-187.

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This study evaluated comparatively the adhesion of Epiphany and AH Plus endodontic sealers to human root dentin treated with 1% NaOCl and 1% NaOCl+17% EDTA, using the push-out test. Sixty root cylinders obtained from maxillary canines had the canals prepared and were randomly assigned to 3 groups (n=20), according to root dentin treatment: GI - distilled water (control), GII - 1% NaOCl and GIII - 1% NaOCl+17% EDTA. Each group was divided into 2 subgroups (n=10) filled with either Epiphany or AH Plus. Bond strength push-out test data (kN) were obtained and analyzed statistically by ANOVA and Tukey's post-hoc test. There was statistically significant difference between sealers (AH Plus: 0.78 ± 0.13; Epiphany: 0.61 ± 0.19; p<0.01) and among root dentin treatments (distilled water: 0.58 ± 0.19; 1% NaOCl: 0.71 ± 0.12; 1% NaOCl+17% EDTA: 0.80 ± 0.17; p<0.05). In conclusion, AH Plus sealer presented greater adhesion to dentin than Epiphany, regardless of the treatment of root canal walls.

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ABSTRACTObjectives: CAD-CAM system is popular because of high esthetic and short fabrication time. But, there is limited information available about the microtensile bonding of luting cements to CAD-CAM inlays and to dentin. The aim of this study was to examine the bond strength of CAD-CAM (Cerec 3) and pressed-ceramic (IPS Empress 2) inlays to dentin surface by microtensile testing using two luting cements.Materials and Methods: Standardized mesio-occlusal cavities were made in forty extracted molar teeth. An occlusal reduction of 2 mm was made; the bucco-lingual width of the proximal boxes was 4 mm, the occlusal width 3 mm and the depth of the pulpal and axial walls 2 mm. The proximal boxes were extended 1 mm below the cemento-enamel junction. Teeth were randomly assigned to 2 groups to evaluate the bonding of 2 ceramic systems, Cerec 3 (Group I) and IPS Empress 2 (Group II), to dentin. Each of the 2 groups were further divided into 2 luting cement groups, Panavia F (Group A) and Variolink II (Group B). After cementation, the teeth were sectioned into two 1.2x1.2 mm wide ‘I’ shape sections. The specimens were then subjected to microtensile testing at a crosshead speed of 1 mm/min. Twoway ANOVA and Tukey HSD tests were used to evaluate the results.Results: The mean microtensile bond strengths of Cerec 3 and IPS Empress 2 bonding to dentin with luting agents in MPa were Panavia F (13.98±3.44), Variolink II (14.19±3.12) and Panavia F (15.12±3.15), Variolink II (15.45±3.08) respectively. No significant differences were found among the 2 ceramic systems (P>.05) and 2 luting cements with regard to dentin bond strengths (P>.05).Conlusions: There was no difference found between the dentin bond strength of the Cerec 3 and IPS Empress 2 inlays cemented with two luting cements. (Eur J Dent 2007;2:91-96)

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This in vivo study compared the effect of mineral trioxide aggregate (MTA), IRM, Super EBA and ZOE in a puttylike consistency, used as retrofilling materials, in the healing process of periapical tissue of pulpless dogs' teeth submitted to a conventional retrofilling technique. Twenty-four premolars obtained from three dogs were used. At the first intervention, the animals were anesthetized, coronal access was obtained and pulpectomy was done. Root canals were kept open to the oral environment for 180 days to induce the formation of apical lesions. After surgical removal of the lesions with curettes, 2 mm of the apical root was cut out perpendicular to the long axis of the teeth, and root-end cavities were shaped with a low-speed round bur. The bone cavities were irrigated and dried, and the root-end cavities were filled with MTA, IRM, Super EBA and ZOE in a puttylike consistency. The bone cavities were passively filled with blood and flaps were sutured. The coronal access openings were cleaned and double-sealed with ZOE and amalgam. After 180 days, the animals were killed by anesthetic overdose, maxilla and mandible were removed and the pieces were processed for histomorphologic analysis. Data were evaluated blindly on the basis of several histopathologic events and the scores obtained were analyzed statistically using the Kruskal Wallis test. No significant differences were observed among MTA, Super EBA and IRM (p>0.05). However, ZOE had a significantly more negative influence on the apical healing (p<0.05). In conclusion, MTA, Super EBA and IRM had similar histopathologic effects among each other and better performance than ZOE used in a puttylike consistency. Furthermore, only MTA stimulated hard tissue deposition in direct contact with the retrofilling material, even when it was inserted under critical conditions.

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Both carbon dioxide (CO2) and water (H2O) are triatomic molecules that are ubiquitous in nature, and both are among the five most abundant gases in the Earth’s atmosphere. At low temperature and ambient pressure, both CO2 and H2O form molecular crystals––dry ice I and ice Ih. Because water possesses distinctive hydrogen bonds, it exhibits intricate and highly pressure-dependent phase behavior, including at least 17 crystalline ice phases and three amorphous ice phases. In contrast, due to its weak van der Waals intermolecular interactions, CO2 exhibits fewer crystalline phases except at extremely high pressures, where nonmolecular ordered structures arise. Herein, we show the molecular dynamics simulation results of numerous 2D polymorphs of CO2 molecules in slit nanopores. Unlike bulk polymorphs of CO2, 2D CO2 polymorphs exhibit myriad crystalline and amorphous structures, showing remarkable polymorphism and polyamorphism. We also show that depending on the thermodynamic path, 2D solid-to-solid phase transitions can give rise to previously unreported structures, e.g., wave-like amorphous CO2 structures. Our simulation also suggests intriguing structural connections between 2D and 3D dry ice phases (e.g., Cmca and PA-3) and offers insights into CO2 polyamorphic transitions through intermediate liquid or amorphous phases.

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Background. In modern dental practice, there is a problem of complications after treatment of caries, such as the postoperative sensitivity of the tooth, formation of secondary caries, and caries complications in the form of pulpitis or periodontitis of the tooth. In addition, this problem exists despite the achievements in the production of high-quality composite filling materials, which are very popular among dentists worldwide. One of the problems that lead to complications after caries treatment is a violation of the integrity of the adhesive bond between the composite and the tooth tissue, which is manifested by a violation of the seal of restoration and the formation of a gap between the filling material and the tooth tissues. This in turn leads to the development of tooth sensitivity and the possibility of bacterial invasion between the filling material and the walls of the tooth, followed by the progression of the pathological process. In the scientific literature, there is evidence of the positive effect of preheating and vibration on the composite before its polymerization. Aim. Additionally, the purpose of this study is to determine the effect of physical impact, such as heating and vibration, on the composite using ultrasound in order to improve the quality of the adhesive fixation of the composite filling material to the walls of the tooth. Materials and methods. The study was conducted on the extracted teeth of patients. Based on this, we have proposed a method for the simultaneous thermo-vibration action on a non-polymerized composite directly in the formed tooth cavity to improve the physicochemical, including the adhesive, properties of light-curing composites. Results of the study revealed a statistically significant positive effect of thermo-vibration on the composite, not only in comparison with the classical method of working with the composite at room temperature, but also with the method of preheating the composite in a furnace used for heating the composite.

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This study aimed to evaluate by the intra-osseous implant technique the most commonly used materials for pulp therapy in pediatric dentistry: calcium hydroxide (CH), Guedes Pinto paste and CTZ paste, according to FDI (1980) and ANSI/ADA (1982) recommendations. Thirty guinea pigs, 10 for each material, divided into experimental periods of 4 and 12 weeks received one implant on each side of the lower jaw symphysis. The external lateral tube wall served as control for the technique. At the end of the observation periods, the animals were euthanized and specimens were prepared for routine histological examination. It was observed that CH and CTZ paste induced severe inflammation, a large amount of necrotic tissue, lymphocytes, foreign body cells and bone resorption, while Guedes Pinto Paste induced little or no inflammation in the 4-week observation period. After 12 weeks, the reactions to CH and Guedes Pinto paste were also absent/mild, presenting a general pattern of replacement by recently formed bone tissue while a moderate to severe inflammatory response was observed with CTZ paste. Guedes Pinto paste presented acceptable biocompatibility levels in both analyzed periods; CH only showed acceptable biocompatibility in the 12-week period while CTZ paste showed no biocompatibility in both periods. Among the tested materials, only Guedes Pinto paste presented an acceptable biocompatibility.

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Multi-leaf stone masonry walls are among the most vulnerable elements of historic constructions. Grout injection is a common and efficient technique to consolidate such masonries. It consists of introducing a grout into the masonry inner core in order to upgrading the cohesion of the wall by ensuring the transversal bond between the external leaves and improving its monolithic behaviour. Notwithstanding, the recrystallization of salts due to changes in moisture content causes several damages in these masonries, even after the consolidation intervention. This paper aims to assess the potential use of linseed oil in natural hydraulic lime-based grouts to mitigate the water penetration and therefore the damages from salts crystallization. Linseed oil was used in former times as an additive for mortars in order to grant hydrophobicity. In this study several properties of the grouts were evaluated: rheology, mechanical strength, water absorption, adhesion and durability assessed by testing the resistance to sodium chloride. Moreover, this paper also analyses the correlation between non-standard specimens (with reduced size) and standard specimens (40x40x160 mm3). The experimental results revealed that the grouts durability and water transport are significantly improved with added linseed oil. It was also possible to observe a small reduction in mechanical resistance with the presence of linseed oil; however, acceptable strength values to promote an appropriate consolidation were ensured. Furthermore, the reduced size specimens revealed to be a viable alternative to the standard ones.

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ABSTRACT Cell surface hydrophobicity (CSH) of Candida species enhances virulence by promoting adhesion to host tissues. Biochemical analysis of yeast cell walls has demonstrated that the most significant differences between hydrophobic and hydrophilic yeasts are found in the acid-labile fraction of Candida albicans phosphomannoprotein, suggesting that this fraction is important in the regulation of the CSH phenotype. The acid-labile fraction of C. albicans is unique among fungi, in that it is composed of an extended polymer of β-1,2-mannose linked to the acid-stable region of the N-glycan by a phosphodiester bond. C. albicans serotype A and B strains both contain a β-1,2-mannose acid-labile moiety, but only serotype A strains contain additional β-1,2-mannose in the acid-stable region. A knockout of the C. albicans hom*olog of the Saccharomyces cerevisiae MNN4 gene was generated in two serotype B C. albicans patient isolates by using hom*ologous gene replacement techniques, with the anticipation that they would be deficient in the acid-labile fraction and, therefore, demonstrate perturbed CSH. The resulting mnn4Δ-deficient derivative has no detectable phosphate-linked β-1,2-mannose in its cell wall, and hydrophobicity is increased significantly under conditions that promote the hydrophilic phenotype. The mnn4Δ mutant also demonstrates an unanticipated perturbation in the acid-stable mannan fraction. The present study reports the first genetic knockout constructed in a serotype B C. albicans strain and represents an important step for dissecting the regulation of CSH.

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Mechanical and physical properties of medium-density fibreboard (MDF) panels made from black spruce ( Picea mariana (Mill.) BSP) top, middle, and butt logs were studied. The analysis of variance and analysis of covariance were both performed to examine the impact of log position in the tree on panel modulus of rupture (MOR), modulus of elasticity (MOE), internal bond (IB), linear expansion (LE), thickness swell (TS), and water absorption. Results indicate that MOE and IB strength of MDF panels made from top and middle logs were significantly superior to those of panels made from butt logs; however, there was no significant difference in MOE and IB between panels made from top and middle logs. Water absorptions of top and middle log panels were significantly lower than that of panels made from butt logs, and the difference in water absorption between panels made from top and middle logs was not significant. TS of top log panels was the smallest among the panels from the three log positions in the tree and was significantly different from those of middle and butt log panels. TS of butt log panels was the highest, which was significantly different from that of top and middle log panels. The differences in LE among the panels made from top, middle, and butt logs were not significant. The comparison of MOR of top, middle, and butt log panels was dependent on panel density because of the interactions among the three groups. Top and middle log panels showed superior properties, because the thinner cell walls of fibres from top and middle logs resulted in an increased compaction ratio compared with the butt log panels. Panel density affected both panel MOR and MOE considerably; however, its impact on IB, LE, TS, and water absorption was not significant in this study. The equations describing the linear relationships between MOR, MOE, and panel density were developed.

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Pedicle screws have been widely used for the treatment of spinal diseases, but improper screw placement is not uncommon and may lead to neurovascular injuries and reduced screw fixation strength. This study aimed to investigate the feasibility of using real-time screw insertion torque monitoring to prevent screw penetration. Commercially available synthetic L4 vertebrae were divided in to seven test groups based on different screw placements. Screw insertion torque and maximal pullout strength were compared among groups. The results indicated that the insertion torque gradually increased when the screw tip was within vertebral cancellous bone without penetration. However, an instantaneous decrease of torque value was observed once the screw tip penetrated the cortex wall. When compared to the control group, higher pullout strength was found for the groups with medial cortex penetration. However, vertebrae with medial cortex penetration may lead to the concern of neurovascular damage. Meanwhile, lower pullout strength was found for the groups with lateral cortex penetration and end-plate penetration, which may lead to the concern of screw loosening. We concluded that pedicle screw penetration can be judged using real-time screw insertion torque monitoring during surgery, which may aid surgeons in avoiding neurovascular injury and reduction of screw fixation strength.

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Pre-treatments applied before biological sludge utilization technologies aim to modify the sludge structure for enhanced disintegration degree and biodegradability. Among the thermal pre-treatments methods, microwave irradiation is suitable to degrade the polymeric structure of sludge, and to increase the solubility of organic matters. Energetic efficiency of microwave heating is mainly determined by the dielectric properties, such as dielectric constant and dielectric loss factor. Dielectric properties are influenced by the frequency, temperature; composition and consistency of irradiated material, state and bond of water etc. Therefore, physicochemical changes of sludge structure; e.g. hydrolysis of macromolecules, degradation of cell wall of microorganisms, aggregation of particles; contribute to the change of dielectric parameters, as well. In our work we investigated the correlation between the dielectric parameters and structural change and biodegradability indicators. In the case of municipal wastewater, the change of organic matter removal efficiency during wastewater purification technology at a wastewater treatment plant can be detected by the change of dielectric constant. Results related to sludge processing show, that change of organic matter solubility and aerobic biodegradability correlate the change of dielectric loss factor and dielectric constant. With the degradation of polymeric structure of sludge matrix and decomposition of macromolecules caused by thermal effects or chemical pre-treatments led to increased mobility of ions and enhanced polarization of molecules. These effects led to increased dielectric constant and loss factor, what make possible to pre-indicate the efficiency of sludge pre-treatment processes by an in line and real time measurement method.

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Abstract The aim of this study is to verify the fracture resistance of premolars with large mesiocclusodistal (MOD) preparations with composite resin using different incremental techniques when subjected to an occlusal load. Forty maxillary premolar teeth were randomly divided into four groups (n=10). Class II MOD cavities were prepared in all specimens with parallel walls and no approximal boxes. The resulting isthmus width was 1/3 the distance between the cusp tips and 3/4 the height of the crown. Teeth in group I, the control group, were not restored. Specimens in group II were restored in three incremental vertical layers. Group III specimens were restored in three horizontal layers, and finally, specimens in group IV were restored in oblique layers. With exception of the placement technique, specimens in groups II, III and IV were restored using the Single Bond adhesive system and P60 composite resin following manufacturer's recommendations. A 4 mm diameter steel sphere contacted the buccal and lingual cusps of the tested teeth at a crosshead speed of 0.5 mm/min until fracture occurred. The values obtained in this study were subjected to Analysis of Variance (ANOVA) and a Tukey–Kramer test. Only group I (non-restored) obtained a minor means of fracture resistance. No significant differences among groups II, III, and IV were found. This study shows on large MOD cavities the incremental filling techniques do not influence the fracture resistance of premolar teeth restored with composite resin. Citation França FMG, Worschech CC, Paulillo LAMS, Martins LRM, Lovadino JR. Fracture Resistance of Premolar Teeth Restored with Different Filling Techniques. J Contemp Dent Pract 2005 August;(6)3:062-069.

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Abstract We evaluated microvessel area of bone marrow (BM) samples from 20 patients with advanced CLL (i.e., symptomatic Binet stage A, B or C) who received at least 8 weeks, after the end of treatment with fludarabine, subcutaneous alemtuzumab, three times weekly for 6 weeks, at escalating dose up to 10 mg. The patient sample included 14 males and 6 females with a median age of 51 years (range 44–60). After a median number of 6 cycles of fludarabine (range, 4–13), 11 (55%) patients could be classified in complete remission (CR) and 9 (45%) in partial remission (PR) (7 nodular-PR and 2 PR). Interestingly, the rate of CR increased to 90% (18 CR; P=0.03; Fisher’s exact test) after treatment with alemtuzumab. In keeping with hematological responses, significant changes in BM microvessel area were observed The decrease of BM angiogenesis, observed virtually in all patients, was a continuous process characterizing the sequential use of fludarabine and alemtuzumab (P = 0.002; Kruskal-Wallis test). This conspicuous feature was easily demonstrable in either ZAP-70-positive (P = 0.02) or ZAP-70-negative (P = 0.001) patients. Residual disease was assessed using a consensus polymerase chain reaction (PCR) methodology to detect the clonality of IgH sequences. Thirteen out of 20 (65%) patients changed from a monoclonal to a polyclonal pattern of IgH after alemtuzumab consolidation. We wondered whether molecular response could translate into a change in the BM microvessel area. A separate evaluation carried out in patients with a persistent monoclonal pattern and in those who changed to a polyclonal pattern of IgH after alemtuzumab therapy showed a significant reduction of BM microvessel area only in the latter (P = 0.0002). A new information provided by the present study concerns the impact of the cumulative dose of alemtuzumab on either molecular response or reduction in BM angiogenesis. Analysis carried out after setting as cut-off the median cumulative dose of alemtuzumab (i.e., 184 mg; range, 134–187) revealed a dose-response relationship. Only two (15.3%) out of 13 patients who received a cumulative dose of alemtuzumab higher than 184 mg had persistent monoclonal IgH in comparison to 5 (71.4%) out 7 patients who had received a cumulative dose of alemtuzumab lower than median (P = 0.007). Similarly, a significant decrease in the extent of microvessel area was observed among patients whose cumulative dose was equal or higher than 184 mg (P = 0.0001) while the same did not apply for a cumulative dose of alemtuzumab lower than 184 mg (P = 0.09). Overall, these data demonstrate a decrease in BM vascularity after treatment with alemtuzumab, which reflects both molecular response and cumulative dose of this drug, and suggest a potential use of alemtuzumab as anti-angiogenic agent in the treatment of advanced CLL.

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Aim: The purpose of this study was to evaluate the effectiveness of different adhesive systems in amalgam restorations and their effects on microleakage. Methodology: In this study, 105 caries-free extracted human permanent molar teeth were used. Teeth were randomly assigned to five groups (n=21), and class I cavities were created on the surface of each tooth. The first was a control group to which no adhesive system was applied. Amalgam Liner (VOCO GmbH, Cuxhaven Germany) was applied to Group II, Clearfil SE-Bond (Kuraray Europe GmbH, Frankfurt Germany) was applied to Group III, Panavia F 2.0 (Kuraray Europe GmbH, Frankfurt Germany) was applied to Group IV, Amalgambond Plus (Parkell Inc.Edgewood, NY USA) was applied to Group V, and then amalgam (Tytin, Kerr, California USA) restorations were placed. After the polishing process, samples were subjected to thermocycling 1,000 times. Teeth were sectioned bucco-palatinally/lingually, and microleakage scores of the occlusal walls were evaluated under a stereomicroscope at 15X magnification by a standardized scale ranging from 0 to 4. One tooth was selected randomly from each group for SEM (Scanning Electron Microscope), and SEM LEO EVO 40 (LEO Ltd., Cambridge UK) photographs of amalgam-tooth hard tissue interfaces were also taken at different magnifications. The results of the microleakage tests were statistically analyzed by both the Kruskal-Wallis Test and the Mann Whitney U Test. Results: In terms of microleakage among groups, the differences that were determined were significant (p<0.05). Microleakage within the control group was determined to be the highest, and statistically important differences were observed between the other groups. Group V (Amalgambond Plus) was determined to have the lowest microleakage scores. Conclusion: In prepared class I cavities, amalgam adhesive systems are effective in preventing occlusal microleakage but do not completely blocked it. How to cite this article: Ünal M, Atakul F. The evaluation of effectiveness of adhesive systems on dental amalgam restorations. Int Dent Res 2021;11(2):83-92. https://doi.org/10.5577/intdentres.2021.vol11.no2.5 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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The objective of this document is to review the clinical applicability of coronary artery calcium (CAC) scoring in both asymptomatic and symptomatic patients at risk for cardiovascular disease. We begin by describing the pathological basis of atherosclerosis, the characteristic stages of atherosclerotic plaque development, and the mechanism and role of arterial calcification in advanced atherosclerotic lesions. We also explain the utility of CAC scoring in cardiovascular risk assessment, discuss the most current clinical methods for measuring CAC, and examine major clinical studies reporting CAC scores in both asymptomatic and symptomatic heart patients. Lastly, the current recommendations for CAC scoring as stated by the American College of Cardiology/American Heart Association (ACC/AHA) are outlined, and a number of considerations for future research are provided. Atherosclerosis begins when certain factors cause chronic endothelial injury, which eventually leads to the build up of fibrofatty plaques in the intima of arterial blood vessels. In time, blood vessel walls can weaken, thrombi can form and plaques can send emboli to distal sites. There are six characteristic stages of plaque development. Mature plaques may be calcified in an active process comparable to bone remodeling, where calcium phosphate crystals coalesce among lipid particles inside arterial walls. Calcification is only present in atherosclerotic arteries, and the site and levels of calcium are non-linearly and positively associated with luminal narrowing of coronary vessels. Calcification is also postulated to stabilize vulnerable plaques in atherosclerotic vessels. Recent studies have shown that CAC scoring can improve the management of both asymptomatic and symptomatic heart patients. Electron beam computed tomography (EBCT) and Multidetector computed tomography (MDCT) are two fast cardiac CT methods used to measure CAC. No matter what technique one uses, CAC is scored with either the Agatston or the “volume” score system. The ACC/ AHA currently finds it is reasonable for asymptomatic patients with intermediate Framingham risk scores (FRS) to undergo CAC assessment because these patients can be re-stratified into the high risk category if their CAC scores are ≥400. Conversely, CAC measurement in asymptomatic patients with low or high FRS is not warranted. There is also no evidence to suggest that high risk asymptomatic patients with no detectable coronary calcium should not be treated with secondary prevention medical therapy. For symptomatic patients, the ACC/AHA recommends CAC assessment as a second line technique to diagnose obstructive CAD, or when primary testing modalities are not possible or are unclear. Furthermore, they do not recommend the use of CAC measurement to determine the etiology of cardiomyopathy, to help identify patients with acute MI in the emergency room, or to assess the progression or regression of coronary atherosclerosis. Future research needs to incorporate calcium scores with percentile rankings, larger population samples, more women with at least intermediate Framingham risk, sufficient numbers of non-Caucasians, reports on cost-effectiveness, and data on populations with Chronic Kidney Disease, End Stage Renal Disease and Diabetes.

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Systematical archaeological excavations at the site Gamzigrad - Felix Romuliana continued in 2007-2008 in the south-eastern part of the fortified imperial palace, in the section of the thermae according to the plan of archaeological research for this site (2005-2009). In 2007, squares L'XXIV, M'XXIV, M'XXIH and M'XXII, which were investigated in 2005 to the horizon c, dated to the end of the 5th and the beginning of the 6th centuries, were completely excavated to the level of the porch of the earlier fortification of Romuliana (Plan 1). The stratigraphy of the cultural layers in these squares is as follows (Fig. 1): Below horizon c there is a layer of construction rubble mixed with brownish-yellow, clay like, sandy soil, 50-75 cm thick, comprising the finds dated in the last quarter of the 4th-5th centuries, layer D; The level of layer D is horizon d, where a structure destroyed in a conflagration, house 1/07, was discovered in squares M'XXII and M'XXIII. It could be dated, on the basis of the preserved household (pottery, metal and antler items, coins, etc.), from the last quarter of the 4th to the middle of the 5th century; Horizon d 1 is a mortar floor discovered beneath horizon d, which presents the earlier phase of house 1/07; Horizon d 2 is the earliest mortar floor inside the house 1/07, covered with a later mortar floor (horizon d 1) and a levelling layer of yellow sand and gravel, which comprises the finds dating also to the last quarter of the 4th to the middle of the 5th centuries; Layer E, 15-40 cm thick, is below horizon d, comprising dark brown soil with rubble and lenses of soot at the bottom, together with finds dated to the second half of the 4th century; Horizon e is covered with layer E, and spread across all the squares which were investigated to the south and to the east of Galerius' bath, where 8 large postholes, which outlined a space 7 x 3 m large and probably some kind of porch, were found along with two furnaces and two pits; Layer F, about 30 cm thick, is the substructure of horizon e and it comprises crushed stone and pebbles mixed with lime mortar, and in places has a levelling of reddish-brown sand. Finds here were dated to the end of the 3rd and the first half of the 4th centuries; Horizon f is a mortar floor of the later fortification of Felix Romuliana at a level of 184.75 m in the west and 184.55 m in the east (an average level of 184.64 m), which was interrupted by a trench running in an east-west direction along the southern section of squares L'-M'XXIV. The trench was filled with soot, small rubble and reddish-brown sand and comprised a large amount of artifacts, such as pottery and glass fragments, metal and bone items and coins dated to the second half of the 3rd century (Fig. 4). Layer G consists of dark brown and yellowish-brown clay with small rubble and soot. It was a levelling layer above the intense construction rubble from the previous horizon and a substructure of horizon f. This layer comprised archaeological finds dated to the end of the 3rd and the first half of the 4th centuries and to the prehistoric period (Early Iron Age); Horizon g is a mortar floor of the porch of the southern and eastern rampart of the earlier fortification of Romuliana. 4 pillars of the eastern porch (pillars 1-4, discovered in 2004-2005), a corner pillar in an L-shape (pillar 5) and one pillar of the southern porch (pillar 6) have been ascertained. From this level the water and sewage canals were dug (Fig. 5). In squares K'XXII-XXIII a trench, measuring 4 x 2 m, in an east-west direction, was opened which aimed to investigate the layers beneath the Roman horizon g. The stratigraphy in this trench is as follows: - Layer G at a level of about 184.53 m; - Layer H, about 35 cm thick, is greenish-yellow clay in which Roman canals were buried, comprising the fragments of the Early and Late Iron Age pottery and fragments of reddish rammed earth (Fig. 2); Layer I, about 20cm thick, is greenish-brown clay, comprising the scarce fragments of the Early and Late Iron Age pottery; Virgin soil consists of yellow clay starting from a level of 184.00 m in the west and of 183.60 m in the east. In 2008, the remains of an earlier building were discovered beneath the floor of the apodyterium of Galerius' bath found in 2002 and below the foundation of the sudatorium and the tepidarium of the same structure, which were found in 2005. Also, for the purposes of conservation and restoration of the thermae, an apsidal room next to the west wall of the apodyterium, so called 'Galerius' dressing room', was completely filled with construction rubble, among which was found a part of an abraded vault (Fig. 6). Excavations proved that the apsidal room had been a pool with cold water, a frigidarium, which was twice renovated and was decorated with mosaic made of black, white and grey stone cubes (Fig. 7). The phases of reconstruction of the frigidarium could also be noticed in its eastern wall (Fig. 8). Also in the rubble inside the pool, glass mosaic cubes of deep blue and golden colours were discovered, indicating the decoration of the vault. In the latest phase, two pillars were constructed to carry the stairs made of stone slabs (Fig. 8). The earliest phase of this room, which had a rectangular layout and a mortar floor, could be part of the building dating back to before Galerius' bath (Plan 2). During the cleaning of the eastern wall of the frigidarium, a semicircular niche with a fresco decoration of geometrical and figural motives, painted in black, dark red, orange and blue on an ochre surface, was discovered (Fig. 3). Under Galerius' bath, a large earlier building was investigated (trenches 1-5/08). Only its foundation zone is preserved. The walls of the Imperial bath were founded on the earlier walls, which were 0.65 m thick and had foundations which were 0.90 m thick (Plan 2). The pilaster of the west faeade of the thermae was also founded on the earlier wall, but it destroyed a water canal (canal A discovered inside the south room of Galerius' bath in 2004), which was constructed after the earlier structure and before the Imperial bath (Fig. 9). It is interesting that the part of the earlier building to the west of the thermae was not demolished during the construction of the Imperial residence. It was adapted and incorporated into the plan of the fortified palace. The original construction was a large public building, probably theprincipia, with a row of rooms around a large courtyard, the atrium. The entrance, which had a porch and a pylon with two square towers and thresholds made of stone slabs, was in the north. (Figs. 10-14) Previously, this building was mistakenly dated to the 4th-5th centuries, because it had been reused in Late Roman and Early Byzantine periods. (Figs. 15-18) However, based on the results of the new research, it could be dated to the 3rd century. .

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Abstract Antigenic expression during erythroid maturation is not well understood. Despite the large number of surface antigens currently described for leukocytes, only a few (e.g. CD36, CD45, CD71, CD117, and CD235a) have been well characterized on erythroid cells. Here, we apply novel bioinformatic tools to select antigens with high potential for identifying successive stages of erythroid maturation in an unsupervised and unbiased manner from a high dimensional dataset. In brief, flow cytometry was performed on 3 normal donor bone marrows using Becton-Dickinson lyoplates containing a total of 275 unique antibodies and 8 gating reagents (CD15, CD19, CD34, CD38, CD45, CD71, CD117, and CD123). Each of the 275 independent data files from the same donor was aligned using the gating reagents and a weighted nearest neighbor algorithm in order to synthesize a flow data set with 283 antigens and 50,000 events. We applied a modified SPADE algorithm to generate a maturational path for cells of the erythroid lineage starting with CD34+/CD38- progenitors through the mature erythrocyte stage. A non-parametric Kruskal–Wallis test was used to identify and rank antigens that show differential expression along the erythroid maturational sequence. To discern antigens having the greatest discrimination for early vs. late erythroid maturation, we identified 15 antigens common to the top 25 most differentially expressed antigens from each donor sample. As expected, our method correctly identified the three erythroid gating reagents (CD45, CD71 and CD117) as well as previously a previously described erythroid associated antigen (CD36). A few of the identified antigens have been described are less common but also known to be differentially expressed on erythroid cells (e.g. integrin members CD29, CD44, and CD49d), however several additional novel antigens were also identified with strong differential expression including CD46, CD58, CD81, CD98, CD99, CD164, CD220 and CD321 (Figure 1). Of note, CD49d, CD98 and CD164 are of particular interest as they appear to be gradually lost during maturation from the early normoblast through the mature erythrocyte stages. This is in contrast with the other antigens that show high expression on pronormoblasts—with rapid decline during the early normoblast stage to the level of erythrocytes. We examined selected antigens from the synthetic data of all 3 samples using manual gating, and interestingly CD99 retained expression among the erythroid cells to late stage normoblasts in 2 of the 3 samples, suggesting some element of phenotypic variability among normal individuals for this antigen. Additionally, several antigens appear useful in distinguishing erythroid lineage cells from non-erythroid precursors including beta-2 microglobulin, CD50, and HLA-ABC which all showed ubiquitous expression in non-erythroid precursors with low to absent expression among cells of the erythroid lineage. In summary, using a novel unbiased method for large-scale antigen discovery, we have identified multiple novel antigens that are differentially down regulated with progressive erythroid maturation and appear useful in further delineating erythroid progenitor maturation. Additional work is underway to correlate these antigens with our morphologic understanding of erythroid maturation. Further work in characterizing these changes in myeloid stem cell disorders is on going and may be of diagnostic utility in the diagnosis of myelodysplastic syndromes. Figure 1 [Red=High Expression, Blue=Low Expression] Figure 1. [Red=High Expression, Blue=Low Expression] Disclosures No relevant conflicts of interest to declare.

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Introduction: The overall survival of children with ALL (Acute Lymphoblastic Leukemia) has improved markedly over the last decade, yet relapse occurs in about 20% of patients. The spread of leukemic blasts to the Central Nervous System (CNS) and increased resistance to therapy due to cell adherence within the Bone Marrow (BM) or CNS constitutes major challenges to treatment. For these reasons, adhesion molecules governing the homing and adhesion of leukemic cells are perceived to be of extraordinary importance, both as potential biomarkers and therapeutic targets. Integrins constitute a large family of heterodimeric receptors composed of alpha and beta subunits, which play important roles during homing and migration of normal leucocytes by facilitating adhesion to both stromal cells and components of the extracellular matrix. Increased expression of CD49d (integrin subunit α4) is a marker for adverse prognosis in ALL and recently, CD49f (integrin subunit α6) was shown to facilitate metastasis of ALL xenografts to the central nervous system in mice (Yao H et al., Nature 2018). Methods Previous studies of integrins in BCP-ALL have focused on individual alpha integrins in xenograft models and were based on limited numbers of clinical samples. The present study was based on a large number of Danish pediatric BCP-ALL patients stratified between 2012-2018 using Minimal Residual Disease (MRD) according to the Nordic NOPHO-2008 protocol (Toft N et al., Eur J Haematol 2013). Diagnostic BM samples were subjected to flowcytometric analysis (FCM) of CD49f (n=246) and CD49d (n=135), using a backbone of lineage-specific B-cell markers (CD45, CD10, CD19, CD20). Leukemic blasts were detected in Cerebrospinal Fluid (CSF) using high-sensitivity FCM and the following markers CD45, CD10, CD19, CD20, CD34 and CD38 (n = 246, with matching BM and CSF samples). Results Our data provided us with a unique possibility to identify the role of CD49d and CD49f with respect to minimal residual disease (MRD) at the end of induction therapy (day 29), which is considered the most important prognostic factor in paediatric lymphoblastic leukemia. We found that CD49f was more highly expressed in patients with MRD ≥ 0,1% at day 29 than patients with MRD < 0,1% (p = 0,01), whereas no difference was seen with respect to CD49d. We also investigated the correlation between white blood cell (WBC) and surface expression of CD49d and CD49f in diagnostic BM blasts with respect to different cytogenetic subtypes. A Kruskall-Wallis test showed that the expression varies according to genetic subtypes (p‹0.0001). We found that the expression of CD49d was highest among the high hyperdiploid and iAMP21 (intrachromosomal amplification of chromosome 21) patients, whereas the expression of CD49f was highest among the t12,21 and iAMP21 patients. Notably, the expression of CD49f was inversely correlated to WBC (r=0,17, p=0.01), which was most pronounced among the patients in the B-other cytogenetic subgroup defined as leukaemia that could not be classified into the existing cytogenetic groups (r=0,42, P‹0.0001). In case of strong adherence to BM, lower levels of leukemic blasts might be expected in circulation resulting in high MRD but low WBC. Therefore, both MRD and WBC data are consistent with a prominent adhesive role of CD49f within BM. In contrast, we found significantly lower CD49f surface expression in diagnostic BM samples in patients with leukemic blasts within CSF (p=0.0297). Conclusions: Recently, Yao et al. (2018) showed that ALL cells in circulation are unable to breach the blood-brain barrier in mice and instead employ CD49f (integrin α6) to migrate into the CNS along vessels that connect vertebral or calvarial bone marrow and the subarachnoid space. Potentially, this mechanism could account for cases of ALL relapse within CNS. Our work shows a strong association between high MRD and the expression of CD49f which is a function that would have been anticipated for CD49d due to previous works with ALL and CLL. Furthermore, we found significantly lower CD49f surface expression in leukemic blasts within the BM in patients with CSF involvement and therefore no support for the recently proposed role of CD49f in facilitating the spread of leukemic cells to the CNS. Disclosures No relevant conflicts of interest to declare.

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Abstract Background Parathyroid hormone (PTH) is synthesized and secreted by the chief cells of the parathyroid gland. PTH has a positive impact on hematopoietic stem cells by indirectly decreasing hematopoietic cell apoptosis, and is currently being investigated as a potential therapeutic to stimulate hematopoiesis and enhance bone marrow engraftment. According to previous study, it was hypothesized that elevated PTH may mediate the induction of multiple myeloma (MM) through the biologic effects of IL-6. However, there was no comprehensive study regarding clinicopathological implications of PTH in MM. This study investigated the pathological and clinical implications of serum PTH in MM patients and relationship with other risk factors of MM. Patients and Methods A total of 115 patients who were newly diagnosed with MM were enrolled between 2006 and 2012. Serum PTH level was measured by automated 2-site chemiluminescent sandwich assay (Abbott Diagnostics). The medical parameters were reviewed for age, sex, PTH, plasma cell percentage in bone marrow, serum M protein, immunoglobulin level, free light chain (FLC) - kappa and lambda, FLC ratio, calcium, creatinine, hemoglobin, albumin, beta-2 microglobulin, lactate dehydrogenase (LDH), international staging system (ISS) stage, international myeloma working group (IMWG) response, chromosome abnormality, bone lesion, treatment outcome, and so on. The collected data was analyzed by PASW 18.0. We performed Spearman’s correlation analysis, Mann Whitney U-test, Kruskall Wallis test, time dependent Cox regression analysis, survival analysis by Kaplan-Meier method, etc. Results Serum PTH level of 115 myeloma patients was 24.7±34.9 (ranged 0.0-284.1) pg/mL. Serum level of IgG, IgM, FLC-lambda, albumin, and LDH was in positive correlation with serum PTH. Age, plasma cell percentage, M protein, IgA, FLC-kappa, FLC ratio, calcium, creatinine, hemoglobin, and beta-2 microglobulin showed negative correlation with PTH. Among those above, IgM (rho=0.190, p=0.045) and calcium (rho=-0.220, p=0.043) revealed statistically significant correlation with serum PTH. Serum PTH level in MM patients was not significantly different according to the group of IMWG response (p=0.450) and ISS stage (p=0.414). Meanwhile, there was no significant difference of PTH according to chromosomal abnormality (p=0.353), bone lesion (p=0.207), and disease progression (p=0.322). Besides, only calcium level was meaningfully different (p=0.016) by the comparison of clinical parameters between MM patient’s group with high PTH level and non-high PTH group (cut-off PTH level=68.3 pg/mL, Mann Whitney U-test). Relative to non-high PTH (<68.3 pg/mL) group, the hazard ratio was higher for group with high PTH level (≥68.3 pg/ml) ([HR] 3.037; p=0.524; 95% CI, 0.100∼92.586; Table 1). With regard to the prognostic implication of serum PTH value, the patient group with above 68.3 pg/mL showed moderate, inferior progression free survival (PFS) than non-high PTH group (median, 5 months vs. 13 months, p=0.056, Fig.1B). However, no overall survival (OS) differences were found between high PTH group and non-high PTH group (Fig. 1A). Interestingly, subgroup analysis showed that patients with high PTH level significantly had an inferior PFS than those with non-high PTH level. In detail, the subgroup were as follows; patients who reached the complete remission (CR) state (N=25) defined by IMWG response criteria at the end of follow up period (median, 3 months vs. 41 months, p=0.001, Fig.1C), patients (N=41) who belonged to ISS stage II (median, 1 months vs. 15 months, p=0.006, Fig.1D), patients (N=97) with no chromosome abnormality (median, 4 months vs. 15 months, p=0.034, Fig.1E), patients (N=28) who have undergone transplantation (median, 3 months vs. 18 months, p=0.009, Fig.1F). Conclusion This study showed that blood PTH level in MM at diagnosis was associated with risk factors and clinical outcome in MM patients. Disclosures: No relevant conflicts of interest to declare.

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Abstract A feature of many integrins is their ability to interact with ligands containing an Arg-Gly-Asp (RGD) motif. RGD-containing peptides and peptidomimetics can inhibit ligand binding to these integrins, including the binding of fibrinogen and von Willebrand Factor (VWF) to the major platelet integrin αIIbβ3. Despite substantial sequence conservation, RGD-containing peptides interact poorly with αIIbβ3 on rat and mouse platelets compared to human platelets, a phenomenon we previously found to be due to sequence differences in the third and fourth N-terminal repeats of human and rat αIIb. To localize the specific residues that control RGD sensitivity and determine whether these residue differences determine sensitivity for all RGD-based ligands, we constructed a series of inter-species chimeric αIIbβ3 molecules in which non-conserved residues in the loops connecting the 2nd and 3rd and the 3rd and 4th blades of the amino-terminal αIIb β propeller were exchanged between the rat and human proteins. We then measured the inhibitory effect of the tetrapeptide RGDS and the tyrosine-based RGD peptidomimetic tirofiban on fibrinogen to the αIIbβ3 chimeras expressed in Chinese hamster ovary (CHO) cells. We chose to study tirofiban because it is a more extended molecule than RGDS with a greater distance between its positively-charged arginine and negatively-charged aspartate moieties. We found that specific substitutions in either the 2–3 loop (a combination of V156Δ, E157S, D159G, and W162G (mature human αIIb numbering)) or in the D232H 3–4 loop affected the sensitivity of rat αIIbβ3 to the inhibitory effects of RGDS alone or in combination. To alter tirofiban sensitivity, only a simultaneous substitution in both loops was effective. Mouse αIIbβ3 is also resistant to RGDS, and this resistance is localized to the same region of the 2–3 loop. There is no D232H in mouse αIIb, and murine αIIbβ3 is as expected as sensitive to tirofiban as its human counterpart. It is of interest that in the published structure of an RGD pentapeptide soaked into the crystal of the related integrin αvβ3 two residues complex with the Arg. These residues are hom*ologous to αIIb D163 and D232, the former located immediately adjacent to the substituted residues in the 2–3 loop that affect RGD resistance and the latter being the important difference between human and rat in the 3–4 loop. Whether D163 and D232 complex to RGDS on αIIbβ3 is unknown. In the published crystal structure of the extracellular portion of human αIIbβ3 co-crystallized with either of several extended RGD-like compounds, the basic residue of the RGD-like compounds was found to hydrogen bond to αIIb D224, a residue located at the base of a pocket that is formed by the 2–3 and 3–4 loops and that is highly conserved among different αIIb species. Our results indicate that sensitivity to such an extended RGD-containing compounds is determined by the composition of the walls of this pocket, thereby facilitating or impeding access to D224. Thus these differences in αIIb composition and function between human and rodent αIIbβ3, which may have been an evolutionary response to venomous exposure by rodents, may provide insights into the intermediate steps by which the αIIbβ3 receptor binds to its natural ligands.

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Abstract Background: Disparities in MM survival among different racial groups persist despite improvements in overall survival (OS). We report a comprehensive review of the SEER Medicare database to elucidate the causes of differences in disease presentation, management practices, cost of care and their relationships to outcomes among the MM patients (pts) from different racial groups. Methods: Pts diagnosed with MM between 1991-2010 with continuous Medicare coverage (1 year prior to diagnosis-date of death/end of 2012) were included. MM complications (MM-C); hypercalcemia (C), renal dysfunction (R), anemia (An), bone fractures (B), dialysis (D) occurring prior to/within 30 days of MM diagnosis (at diagnosis; AT-D), or any time later (after diagnosis; AF-D) were summarized. Demographics, survival (PEDSAF files) and disease characteristics, drug/stem cell transplant (SCT) utilization (NCH, OUTSAF, PDESAF files) were obtained. Trends in incidence of MM-C, treatment type over time (proportional odds) and their association with race (logistic regressions) were compared. Associations between race, MM-C AT-D and MM related death using Fine and Gray's competing risk methods were analyzed. Medicare claims adjusted for inflation (2013) within the first 6 months (mth)/total MM care were summed by drug and total charges. Median cost of care by race and year of diagnosis were evaluated by Kruskal-Wallis (univariate) and proportional odds models (multivariate). Results: A total of 35842 pts (52.1% males) with median age of 76 yr (range 18-104) were included. Mutually exclusive racial subgroups were: White (W, 72%), Hispanic (H: 7.3%), African-American (AA, 16.2%), Asian (A, 4.1%), other (0.3%). A significantly increasing trend in the prevalence of all MM-C AT-D was seen over time (all p<0.001). For AF-D, all MM-C initially increased but decreased from 2006-2010 except D that showed continued increase (Fig 1A). For race, MM-C were significantly different, with AA having the most C, R, An and D while W having the most B (Fig 1B). Inferior OS was seen with male gender (p=0.006), increasing age, earlier years of diagnosis, belonging to W or H race, C, R and B (all p<0.001), but not with An (p=0.298) or D (p=0.378). Trends in OS over time showed a significant interaction with race in 1991-1995 (p<0.001), 1996-2000 (p=0.004) and 2001-2005 (p<0.001) but not in 2006-2010 (p=0.182), possibly due to shorter follow up. Medicare claims analysis showed that pts receiving any treatment increased from 12.8% in 1991-1995 to 59.3% in 2006-2010 (p<0.001). Specific therapy utilization, including SCT and all drug classes/agents evaluated increased except for cytotoxic chemotherapy utilization (melphalan/cyclophosphamide), which decreased (Fig 2A). Any therapy utilization was significantly different by race (p<0.001) with highest for H (36.5%) and lowest for AA (31.6%). Specific treatments differed significantly by race (Fig 2B). Cost of care analysis showed significant increase over years for median drug claims (0.23k to 3.63k) and total claims (5.11k to 27.8k) during first 6 mth as well as for any time after MM diagnosis (drug: 1.3k to 9.9k, total: 21.9k to 84k) (p<0.001 for all). Median drug claims in first 6 mth were highest among AA (1.3k) and lowest among A (0.63k) (p<0.001). Same was true for total claims in first 6 mth (p<0.001). For any time after diagnosis, H had the highest drug (p<0.001) and total (p=0.081) claims. There were significant interactions between year of diagnosis, race and cost of care with H seeing the highest increase in drug claims in first 6 mth (p<0.001) and any time (p=0.002). Conclusion: MM-C have generally decreased AF-D due to improved therapeutics although AA continue to have the highest rates of MM-C. Our data shows that utilization and cost of MM care have increased tremendously with resulting improved outcomes, but significant racial disparities persist. These disparities may be in part due to impaired access to new agents from lack of supplemental insurance for certain racial groups. Drug utilization and cost of care disparities may also be due to differences in co-morbidities, MM-C or access leading to differential healthcare utilization and should be explored in order to ameliorate disparities in outcomes. Disclosures Ailawadhi: Pharmacyclics: Consultancy; Novartis: Consultancy; Amgen Inc: Consultancy; Takeda Oncology: Consultancy.

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Abstract Abstract 4505 Invasive fungal infections (IFI) such as candidiasis and mold infections cause significant morbidity and mortality among immunocompromised patients in recent years. Micafungin, a new echinocandin, inhibits fungal cell wall beta-glucan synthesis, with potent activity against most species of Candida and Aspergillus. The aim of this observational study was to investigate the efficacy and safety of Micafungin in prophylaxis of invasive fungal infections (IFI) in 20 high risk adult patients with various haematological diseases receiving haplo-identical allogeneic stem cell transplantation (Allo-SCT). Treatment success was defined as no proven, probable, or suspected IFI through therapy and the absence of proven or probable IFI through the end of 12 weeks after therapy. (EORTC criteria). These patients were monitored after Allo-SCT for galactomannan antigenemia at least once per week, and a computed tomography (CT) scan was performed in the case of persistent fever for at least 5 days after broad-spectrum empirical antibacterial therapy. Routine controls (e.g. detection of galactomannan antigenemia and CT scan) were performed systematically after 1 and 2 months and on day 100 after Allo-SCT. Only 2 patients had a history of possible aspergillosis before transplant treated by voriconazole. The patients received a median of 4 lines (2–7) of treatments before Allo-SCT. Ten patients (50%) received at least one auto-SCT; and 5 patients (25%) received a previous Allo-SCT. 18 patients (90%) received a reduced intensity conditioning regimen (RIC), with Fludarabine, Cyclophosphamide and Total body irradiation (TBI) 2 Gy based (75%), or Fludarabine, Busulfan, and Cyclophosphamide based (10%) or other (5%). while two patients (10%) received a myeloablative conditioning regimen with thiotepa. The patients and transplant details are shown in the table 1. Patients received a median of 26 infusions (range, 1–8) of Micafungin (50 mg/day i.v. as a 1h infusion). The treatment was initiated at the beginning of the transplant conditioning regimen until the hospital discharge. None of our patients discontinued the treatment for drug-related adverse events. Micafungin was not associated with any hepatotoxicity. Only one patient (5%) discontinued the treatment because an early disease progression. At last follow-up, there are 17 patients (85%) who are alive, with a median follow-up of 6 months (3–17). Three patients (15%) dead because disease progression. In all patients no candidas and/or Aspergillus species was documented after 3 and 6 months from transplant. None of our patients presented a positive galactomannan antigenemia >0.5. Seven patients (35%) presented a CMV reactivation. Only one patient presented an acute GvHD grade II. Micafungin has a good safety and tolerability profile, with an efficacy in preventing IFI in this high risk population. Our data provide support for an efficacy study in a prophylaxis setting, but prospective and comparative clinical trials using Micafungin are urgently needed to define the role of this drug in prophylaxis after haplo-identical Allo-SCT. Table 1. Patient and transplantation characteristics Patient and transplantation characteristics n = 20 (%) Patients Age (median) [range] 40 years (range, 20–60) Patients sex Male 12 (60) Female 8 (40) Disease type HL 6 (30) NHL 5 (25) AML 4 (20) MM 2 (10) MF 1 (5) MDS 1 (5) CLL 1 (5) Median number of prior chemotherapies before Allo-SCT [range] 4 (2–7) Median number of prior Auto-SCT [range] 1 (0–2) 1 8 (40) 2 2 (10) Status of disease at Allo-SCT >2 CR 11 (55) PR 6 (30) PD 3 (15) Median interval between auto and Allo-SCT months [range] Donor type Brother 8 (40) Mother 6 (30) Sister 3 (15) Son 2 (10) Father 1 (5) Donor/recipient sex mis match 9 (45) Conditioning regimen Flu 5+Cy 2+ TBI 15 (75) Flu 5 +Cy 2+ Bu 2 2 (10) Flu 3+TT 2+ Bu 3 2 (10) Flu 6+ Cy 1+ ATG 4 1 (5) GvHD prophylaxis CSA+MMF+Cy 20 (100) Stem cell source Peripheral Blood 11 (55) Bone Marrow 9 (45) Stem cell dose median [range] CD34+ × 106/kg 4,25 (0,8–10,8) CD3+ × 106/kg 140 (17–411) Days with ANC< 500 × 109/l 21 (14–49) Days with platelets<20 × 109/l 26 (14–140) Disclosures: No relevant conflicts of interest to declare.

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Abstract Background. Monoclonal gammopathy of undetermined significance (MGUS) is defined by the presence of a serum M-protein &lt;3 g/dL, bone marrow plasma cells (BMPC) &lt;10% and absence of end-organ damage. The risk of malignant transformation is 1% per year. Size and type of M-protein and an abnormal serum free-light chains (FLC) ratio at diagnosis are reported as the main risk factors for transformation. Aims of the study. To evaluate whether the pattern of presentation of MGUS has changed over the last three decades. Patients and methods. The charts of MGUS patients (pts) diagnosed from 1975 to 2007 were reviewed. The following data were gathered: age, sex, haemoglobin (Hgb), type and size of serum M-protein, uninvolved Ig levels, serum FLC, urine M-protein, BMPC, serum albumin and β2-microglobulin. The study included 1400 pts divided into three groups according to the date of diagnosis: 1975–1987 (group I, 102); 1988–1997 (group II, 380); 1998–2007 (group III, 918). Differences among groups were evaluated using chi-square test for categorical variables and Kruskal-Wallis non-parametric Anova for numerical variables. A P-value ≤0.05 was considered statistically significant. Results., The median age of patients was 63 years (range 20–92), 740 were males and 660 females. The median time from the first detection of M-protein to diagnosis of MGUS was 3.2 months (range 1–264). Serum M-protein was 73% IgG, 13% IgM, 11% IgA, 3% biclonal; light chain was k in 63% of pts, λ in 37%. The serum M-protein was &lt;1 g/dL in 284 patients (21%), 1–1.5 g/dL in 502 (36%), 1.5–2 g/dL in 373 (27%) and ≥2 g/dL in 216 (16%). M-protein size was not reported in 25 cases. The median levels of uninvolved IgG, IgM and IgA were 1350 mg/dL (range 110–7460), 94 mg/dL (range 40–4680) and 162 mg/dL (range 22–2370) respectively. In 236 evaluable pts, median levels of serum FLC k and λ were 17.1 mg/L (range: 1.4–423) and 17.3 mg/L (range: 2–299). Urine M-protein was detected in 19% of pts with a median level of 23.8 mg/L (range 4–450). The median BMPC percentage was 5 (range: 1–10). The median values of Hgb, serum albumin and β2-microglobulin were 14 g/dL (range: 8.6–19.7), 4.3 g/dL (range 2.5–6) and 1930 mcg/L (range 865–44300) respectively. The comparison among the three groups showed statistically significant reduction of serum M-protein levels (p&lt;0.0001), BMPC (p&lt;0.0001), β2-microglobulin (p=0.0001) and increase of Hgb (p&lt;0.0001) and albumin (p=0.0001) over time. In particular, the proportion of pts with a serum M-protein &lt;1 g/dL increased from 4% in group I to 12% in group II and to 26% in group III. A serum M-protein ≥2 g/dL was present in 35%, 22%, 11% of pts in the three groups respectively (p&lt;0.0001). With a median follow-up was 40 months (range: 3–396), corresponding to 7577 person-year, the cumulative probability of malignant transformation was 9%, 18%, 28% at 5, 10, 15 years respectively. Group III pts had a significantly lower 5-year probability of transformation (5%) as compared to groups I and II (20% and 11% respectively). Conclusions. The pattern of presentation of MGUS has changed over time. Patients diagnosed in the last decade have more favourable presenting features as compared to those diagnosed before. This could be due to the availability of more sensitive diagnostic techniques able to detect minimal M-proteins. Another possible explanation is that pts are more frequently and promptly referred by their treating physicians to an hematologic centre, allowing an earlier diagnosis. Both circ*mstances could led to the identification of a subset of pts with different presentation and maybe a better outcome with respect to the MGUS diagnosed in the past decades. This could entail a different approach of physicians in the management of MGUS patients.

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Background Many aspects of lymphoma pathophysiology indicate mutual interactions between the host immune system and lymphoma cells. These interactions may either promote or control lymphomagenesis. An unconventional subset of CD4-CD8- double-negative T cells (DNTs) has been recently described to contribute specifically to anti-tumor immunity. Indeed, DNTs are involved in immune regulation and tolerance as well as in host defense and inflammation, acting as regulatory T cells and/or cytotoxic T cells. DNTs are T lymphocytes which express either αβ or γδ T-cell receptors (TCR) and lack CD4, CD8 and CD56. In healthy human donors and murine models, they constitute about 1-5% of the lymphocytes in peripheral blood and in lymphoid organs. No data are available on the role of DNT cells in human anti-lymphoma immunity. Information from murine models suggests that expanded DNT cells would not impair host immunity against lymphoma and would perhaps stimulate it. DNT cells have also demonstrated to have a direct in vitro anti-tumor activity against lymphoma. Few data are available on the prognostic significance of DNTs in lymphomas, on their interaction with other immune cells, and on their functional attitude. Aims The aim of this study was to assess the frequency and the functional attitude of circulating DNTs in Lymphoma patients and healthy donors as controls, in order to assess the role of DNTs on clinical outcome and progression. Methods To test this population as prognostic factor on clinical outcome and progression of lymphoma disease, peripheral blood (PB) and bone marrow (BM) samples of 46 Lymphoma patients (pts), with non-Hodgkin's Lymphomas and classical Hodgkin Lymphoma were selected and prospectively collected at diagnosis and after one month till the end of chemo- or immuno-chemotherapy therapy. Blood samples were collected also at the time of relapse or progression. As control PB samples of 16 healthy donors were collected. Circulating DNT subsets (TCRαβ+ and TCRγδ+) were characterized for their ontogeny, tolerogenic or cytotoxic attitude and TCR clonality by staining with the following conjugated monoclonal antibodies (MoAbs) for surface and intracellular markers: CD3, CD4, CD8, CD56, CD45, TCRαβ, CD45Ra, CD45Ro, CCR7, CD27, CD28, CD30, CD69, GITR, CD95, CD178, CD152, IFN-γ, TNF-α, granzyme B, and perforin. Isotype-matched MoAbs were used as staining controls. For functional studies, DNTs were purified from PBMCs of patients through a negative selection by using specific MACS microbeads and then cultured for 2 weeks in complete medium supplemented with anti-CD3 (OKT3), rhIL-2 and rhIL-4. Data were acquired using an 8-colour flow cytometer and analyzed using Kaluza software. Data were compared among the groups using the Mann-Whitney non-parametric test or Kruskal–Wallis one-way analysis of variance. The study was approved by the local Ethics Committee and all patients provided their informed consent in accordance with the Declaration of Helsinki. Results The percentage (mean + SE) of DNTs in BM (2.367 ± 0.5891) of Lymphoma pts was lower than in PB samples (3.421 ± 0.981). Moreover we observed a significant decrease (p = 0.006) of circulating αβ-DNTs in pts with untreated lymphoma (23.7 ± 3.7) as compared with healthy controls (31.3 ± 3.4), and their number seemed to be modulated by disease relapse/progression or disease treatment. (fig.1). In Hodgkin's Lymphoma circulating αβ-DNTs were significantly increased as compared with other histotypes (p = 0.0001) (fig.2). Circulating αβ-DNTs were significantly decreased (p=0.006) in serial samples collected after treatment or at the time of disease relapse. Interestingly, after ex vivo expansion, DNTs acquired an immunomodulatory cytokine profile, characterized by the secretion of IFN-γ and granzyme B which are known as central components of anti-tumor immune responses (fig.3). Conclusions To date, no data have been reported on DNT phenotypic and functional characterization in Lymphoma patients. Our study has demonstrated for the first time that αβ-DNTs could play an important role in both the development and the progression of lymphomas. In addition, based on our preliminary results, it is likely that ex-vivo expanded DNTs exert an anti-tumor activity thus suggesting their possible use as a new strategy for adoptive immune-therapy. Disclosures: No relevant conflicts of interest to declare.

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Introduction: The root canal therapy is done with the intention to seal it off from any fluid leaks, thus enhancing the longevity of the therapy. The seal between the obturating material and the dentinal walls of the root surface is done by the endodontic sealer. The sealer is designed in such a way that it has to mould itself with the curves and follow the shapes of the root dentinal wall. Aims: To compare in-vitro the tensile bond strength of four different endodontic sealers to root dentin and Gutterpercha. Methods: Thirty six single ‑ rooted canine and premolar teeth with total apical formation were selected. Working length was determined after the crown portion was cut. BMP was done. Teeth were grouped as Group Ⅰ: MTA‑ based sealer, Group Ⅱ: Bioceramic Sealer, Group Ⅲ: Calcium based sealer, Group Ⅳ: Epoxy resin based (AH Plus sealer). Sealers were manipulated according to the manufacturer protocol. Obtuartion was done using 4% gutta ‑ percha. Later tensile strength test was done on the root slices of 2 mm thickness using universal testing machine. 6 mm in diameter Aluminum cylinders, were stabilized on the gutta–percha with small amounts of wax and were filled with one of the sealers. After setting each sealer, the drops of wax were removed and the tensile bond strengths of all the samples were measured using universal testing machine. The data was analysed using Tukey tests and ANOVA. Results: Among the 4 groups highest bond strength was found in Group Ⅱ (BioRootTMRCS) (P < 0.05) and the least was Group Ⅰ (MTA Fill apex). Conclusion: Bioceramic sealer’s tensile bond strength was highest followed by AH26, Sealapex and MTA‑ based sealer. Keywords: Bond strength; BioRoot TMRCS Sealer; MTA Fill apex; AH Plus Sealer, Sealapex, tensile strength.

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Abstract Background Multiple analytical methods have been developed to determine the ratios of aromatic lignin units, particularly the syringyl/guaiacyl (S/G) ratio, of lignin biopolymers in plant cell walls. Chemical degradation methods such as thioacidolysis produce aromatic lignin units that are released from certain linkages and may induce chemical changes rendering it difficult to distinguish and determine the source of specific aromatic lignin units released, as is the case with nitrobenzene oxidation methodology. NMR methods provide powerful tools used to analyze cell walls for lignin composition and linkage information. Pyrolysis-mass spectrometry methods are also widely used, particularly as high-throughput methodologies. However, the different techniques used to analyze aromatic lignin unit ratios frequently yield different results within and across particular studies, making it difficult to interpret and compare results. This also makes it difficult to obtain meaningful insights relating these measurements to other characteristics of plant cell walls that may impact biomass sustainability and conversion metrics for the production of bio-derived fuels and chemicals. Results The authors compared the S/G lignin unit ratios obtained from thioacidolysis, pyrolysis-molecular beam mass spectrometry (py-MBMS), HSQC liquid-state NMR and solid-state (ss) NMR methodologies of pine, several genotypes of poplar, and corn stover biomass. An underutilized approach to deconvolute ssNMR spectra was implemented to derive S/G ratios. The S/G ratios obtained for the samples did not agree across the different methods, but trends were similar with the most agreement among the py-MBMS, HSQC NMR and deconvoluted ssNMR methods. The relationship between S/G, thioacidolysis yields, and linkage analysis determined by HSQC is also addressed. Conclusions This work demonstrates that different methods using chemical, thermal, and non-destructive NMR techniques to determine native lignin S/G ratios in plant cell walls may yield different results depending on species and linkage abundances. Spectral deconvolution can be applied to many hardwoods with lignin dominated by S and G units, but the results may not be reliable for some woody and grassy species of more diverse lignin composition. HSQC may be a better method for analyzing lignin in those species given the wealth of information provided on additional aromatic moieties and bond linkages. Additionally, trends or correlations in lignin characteristics such as S/G ratios and lignin linkages within the same species such as poplar may not necessarily exhibit the same trends or correlations made across different biomass types. Careful consideration is required when choosing a method to measure S/G ratios and the benefits and shortcomings of each method discussed here are summarized.

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Abstract Background It is known that a number of parameters can influence the post-printing properties of bone tissue scaffolds. Previous research has primarily focused on the effect of parameters associated with scaffold design (e.g., scaffold porosity) and specific scaffold printing processes (e.g., printing pressure). To our knowledge, no studies have investigated variations in post-printing properties attributed to the techniques used to synthesize the materials for printing (e.g., melt-blending, powder blending, liquid solvent, and solid solvent). Methods Four material preparation techniques were investigated to determine their influence on scaffold properties. Polycaprolactone/nano-hydroxyapatite 30% (wt.) materials were synthesized through melt-blending, powder blending, liquid solvent, and solid solvent techniques. The material printability and the properties of printed scaffolds, in terms of swelling/degradation, mechanical strength, morphology, and thermal properties, were examined and compared to one another using Kruskal-Wallis nonparametric statistical analysis. Results Material prepared through the liquid solvent technique was found to have limited printability, while melt-blended material demonstrated the highest degree of uniformity and lowest extent of swelling and degradation. Scaffolds prepared with powder-blended material demonstrated the highest Young’s modulus, yield strength, and modulus of resilience; however, they also demonstrated the highest degree of variability. The higher degree of inhom*ogeneity in the material was further supported by thermal gravimetric analysis. While scaffolds printed from melt-blended, powder-blended, and solid solvent materials demonstrated a high degree of micro-porosity, the liquid solvent material preparation technique resulted in minimal micro-porosity. Conclusions Study results indicate that specific techniques used to prepare materials influence the printing process and post-printing scaffold properties. Among the four techniques examined, melt-blended materials were found to be the most favorable, specifically when considering the combination of printability, consistent mechanical properties, and efficient preparation. Techniques determined to be favourable based on the properties investigated should undergo further studies related to biological properties and time-dependent properties beyond 21-days.

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Abstract Background This in-vitro study aimed to investigate the effect of two different antibacterial disinfectants on the microleakage performance of newly developed bulk-fill composite, bonded to different tooth structures. Methods Class V cavities were prepared in 30 sound premolar teeth, with enamel occlusal margins (OM) and dentin cervical margins (CM). Two disinfectants, 2% chlorhexidine gluconate (CHX) and Listerine Miswak (ListM), were used. Teeth were divided into three groups (n = 10): G1, Control; G2, CHX; and G3, ListM. Disinfectants were applied to the cavity preparation walls after they were etched with 35% phosphoric acid. The Single Bond Universal adhesive system was then used, and teeth were restored with Filtek One Bulk Fill composite. Samples were examined, after thermocycling aging, by stereomicroscopy for the evaluation of marginal dye penetration. Results The highest mean microleakage score was reported in the CM of G1 (2.60 ± 1.174), which was significant compared with that of G2 only (p = 0.02). OM in G1 showed no microleakage, with no significant differences found among groups (χ2 = 1.39, p = 0.50). No significant differences were reported between G2 and G3 (p = 0.45 OM; p = 0.17 CM). Conclusions Cavity pretreatment with CHX is not significantly different to pretreatment with CHX. In contrast, CHX improved the cervical marginal seal as compare to the control group (G1).

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Abstract: A new species of the genus Temnocephala Blanchard, 1849 from southern Brazil was found on two species of anomuran crustaceans, Aegla spinipalma Bond-Buckup & Buckup, 1994 and Aegla grisella Bond-Buckup & Buckup, 1994, the latter classified as a vulnerable species by the "Lista de Referência da Fauna Ameaçada de Extinção no Rio Grande do Sul. Decreto no 41.672, de 11 junho de 2002". The crustaceans were collected from a tributary creek of the Forqueta river, Perau de Janeiro, Arvorezinha and a tributary creek of the Fão river, Pouso Novo, Rio Grande do Sul, Brazil; both localities belong to the Sub-Basin of Forqueta River. The new species differs from seven other temnocephalans epibionts on Aegla Leach, 1820, by having the following characters: 1. a long and slightly curved cirrus, 2. two vagin*l sphincters, one proximal, big and asymmetric, and one distal, smaller and symmetric, and; 3. longer than wide, elongated epidermal 'excretory' syncytial plates (EPs), with a almost horizontally central excretory pore, displaced to the anterior portion of the plate. The new species' EP is the largest in total length among epibionts temnocephalans in crustaceans already registered. Regarding the similarities with the male reproductive system of Temnocephala axenosMonticelli, 1898, the new species has important differences in the female reproductive system. It has a larger proximal vagin*l sphincter, located in the middle of the vagin*, while the smaller distal one is at the extreme end of the organ. Besides that, the vagin*l portion between the proximal and distal sphincters is conspicuous, with a strong muscular wall. This is the first record of a species of Temnocephala in the Taquari Valley, as well in the 'Perau de Janeiro', which is an area with a rich endemic fauna.

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Background: Transthyretin cardiac amyloidosis (ATTR-CA) is an increasingly recognized cause of heart failure with preserved ejection fraction (EF). Bone scintigraphy has emerged as a non-invasive imaging approach that is highly sensitive and specific for identifying ATTR-CA. We conducted a prospective cohort study using 99m Tc-pyrophosphate scintigraphy ( 99m Tc-PYP) to determine the prevalence of ATTR-CA and other clinical associations with 99m Tc-PYP positivity in older patients with severe aortic stenosis (AS) undergoing transcatheter aortic valve replacement (TAVR). Methods: A total of 40 patients (53% men) with severe AS underwent 99m Tc-PYP planar cardiac imaging within 30 days of TAVR. Myocardial uptake was assessed with both a semi-quantitative visual score (range 0: no uptake to 3: uptake greater than bone) and by quantitative analysis by drawing a region of interest over the heart, mirroring it to the contralateral chest, and calculating a heart-to-contralateral ratio (HCL). Semi-quantitative score ≥ 2 and HCL ≥ 1.5 were considered positive for ATTR-CA. Demographics and pre-TAVR clinical history, laboratory values, EKG, and echocardiographic data were also analyzed for association with 99m Tc-PYP positivity. Results: Among 40 patients (mean age 85.2 + 5.3 years), 99m Tc-PYP uptake was found in 10% (n=4), all men. Mean semi-quantitative score was 2.8±0.5 and HCL was 1.6±0.1. Elevated BNP (1379±689 vs 438±483 pg/ml, p=0.02), decreased serum albumin (3.2±0.5 vs 3.8±0.5 g/dL, p=0.02), and both larger interventricular septal wall (1.3±0.4 vs 1.0±0.2 cm, p=0.01) and left posterior wall thickness (1.3±0.6 vs 0.9±0.2 cm, p=0.04) at diastole were associated with 99m Tc-PYP positivity. There was no difference in EF, but myocardial contraction fraction, the ratio of stroke volume to myocardial volume, was significantly lower, 24±12% vs 45±18%, in 99m Tc-PYP positive patients (p=0.03). Conclusions: 99m Tc-PYP has potential as a screening tool for ATTR-CA in high-risk populations. Our data suggest that ATTR-CA may be prevalent in patients undergoing TAVR, particularly men, 20% of whom had a positive 99m Tc-PYP scan. Identifying specific etiologies of heart failure in TAVR patients may be important for prognostication and response to therapy.

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Abstract Introduction Cardiac wild type (wt) transthyretin (ATTR) amyloidos causes hypertrophic, restrictive cardiomyopathy in older patients and is generally considered underdiagnosed. However, its prevalence remains poorly investigated. Scintigraphy using bone tracers, e.g. Tc-PYP and Tc-DPD, is a highly sensitive and specific method for detection of cardiac ATTR and has revolutionised diagnostics The aim of this study was to describe the prevalence of wtATTR amyloidosis in our heart failure population. Methods Our University Hospital is the sole provider of specialised heart care in an area with around 150 000 inhabitants, 37 600 of which are older than 60 years. Medical records for all living patients in the area with a diagnosis of heart failure, hypertensive heart disease or cardiomyopathy between 2010 and May 2018 were scrutinized (n=2237). 95% of these patients had an echocardiographic exam available for re-evaluation. All patients withseptal thickness >14 mm who had no previous DPD scintigraphy or genetic diagnosis of sarcomeric hypertophic cardiomyopathy were offered DPD scintigraphy (n=131). Eight patients died prior to and 38 patients declined work up, mainly due to old age. A DPD uptake of grade 2 or higher was considered diagnostic in absence of signs of AL amyloidosis. Results Adding up patients found during the screening effort and those with an already known diagnosis of wtATTR amyloidosis, 26 patients had wtATTR. Their men age was 84 years and 90% were male. Conclusions The study gives us an estimated prevalence of disease warranting specialized heart failure treatment of 1/1500 in the population older than 60 years. Cardiac wtATTR amyloidosis is common in patients with heart failure and increased LV wall thickness, especially among the older male patients. Therefore we recommend a liberal use of bone scintigraphy in this group.

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Abstract Background Inflammation is a key driver of atherosclerosis and myocardial infarction (MI), and beyond proteins and microRNAs, long noncoding RNAs (lncRNAs) are implicated in inflammation control. To obtain further information on the role of lncRNAs in the context of atherosclerosis, we analyzed transcriptome maps of circulating immune cells (PBMCs) of post-MI patients in whom the lncRNA NEAT1 was suppressed. Here, we report immune disturbances in murine NEAT1 knockout models with wildtype or ApoE−/− genetic background. Methods and results RNA-sequencing (RNA-seq) of PBMCs from post-MI patients revealed profound transcriptome disturbances compared to healthy controls. Among these, NEAT1 suppression was notable since it affected the most highly expressed lncRNA as part of a molecular circuit also encompassing chemokines and interleukins. We used NEAT1−/− mice to evaluate whether NEAT1 depletion per se may cause immune dysfunction. NEAT1−/− splenocytes displayed enhanced baseline ROS production, and RNA-seq identified anomalous expression and regulation of chemokines/ receptors, innate immunity genes, TNF and caspases. FACS revealed displayed anomalous Treg and TH cell differentiation in NEAT1−/− spleens vs. wildtype (WT). Beyond grossly altered transcriptome, NEAT1−/− bone marrow derived macrophages (BMDMs) responded to LPS with increased (p<0.001) ROS production, enhanced baseline phagocytic activity (p<0.001), and attenuated proliferation (p=0.001). FACS revealed deregulated monocyte-macrophage differentiation in NEAT1−/− bone marrow and blood. Further, NEAT1−/− mice displayed aortic wall CD68+ cell infiltration and there was evidence of myocardial inflammation which could lead to severe and potentially life-threatening structural damage in some of these animals. This observation suggests that even stochastic activation of the highly unstable NEAT1−/− immune system may trigger uncontrolled pathogenic cascades, explaining the survival disadvantage of NEAT1−/− mice. In addition to these studies on hom*ozygous NEAT1−/− deficiency in WT background, we obtained data on mice with partial i.e. heterozygous NEAT1−/+ deficiency on ApoE−/− background. Analysis of this new NEAT1−/+ ApoE−/− strain indicates that even partial NEAT1 deficiency leads to systemic inflammation with high IFN-gamma levels, when the animals are exposed to immune stress e.g. high LDL cholesterol. Conclusions Regarding the monocyte-enriched NEAT1 suppressed in post-MI PBMCs, the data from NEAT1−/− and NEAT1−/+ ApoE−/− mice document NEAT1 as a key immune system coordinator whose deficiency affects monocyte-macrophage and T cell differentiation and functions and renders the immune system unstable and highly vulnerable to immune stress. Since in patients NEAT1 is part of a molecular circuit persistently deregulated post-MI, too, it appears reasonable to further search for new therapeutic targets within this circuit, taking advantage of the described genetic animal models.

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ABSTRACTOne-dimensional (1D) nano-materials have attracted a plenty of attention due to their novel structures and properties. Our group has carried out researches on synthesis, structure and property of 1D nano-materials, which are introduced in this paper. First, size effects on the crystal structure of Ag nanowires and on Young's modulus in [0001] oriented ZnO nanowires, respectively, have been revealed and modeled. The former is concerning the systemic energy of an individual Ag nanowire. The latter is caused by the surface stiffening effect arising from surface relaxation induced bond length contractions in the ZnO nanowires. Second, structures of 1D helical nano-materials including SWCNT (single-walled carbon nanotube), B-DNA and MWCNT (mutli-walled carbon nanotube) have been studied. It is shown that there is strong orientation dependence of diffraction intensities from SWCNT and B-DNA, which can even result in certain layer lines missing in their diffraction patterns. Also, it is demonstrated that high-resolution transmission electron microscope (TEM) images of sidewall regions of MWCNTs are not structural ones and from the interference of the {0002} and the {1011} diffraction waves. Third, arrays of four types of 1D heterojunctions have been synthesized. Among these 1D heterojunctions, the interfacial structures of the Ni/MWCNT/a-CNT(amorphous carbon nanotube) heterojunctions show that multiple outer walls in the MWCNTs can simultaneously participate in electrical transport. The electrical properties of the Ni/MWCNT/a-CNT and the Ag/a-CNT heterojunctions have been measured. As a result, it is found that the contacts between the Ag nanowires and the a-CNTs are ohmic ones with universal significance, and that each Ni/MWCNT/ a-CNT contains two diodes connected in series face-to-face. Moreover, most of the diodes have the most nearly ideal characteristics of Schottky contacts, indicated by quantitative analysis with the thermionic emission theory. Last, our group has developed a novel technique for rapidly producing large-area highly-oriented Si nanowire arrays on Si wafers by scratching the Si surface with metal nanoparticles near room temperature in HF solution. By this method, Si nanowires with desirable axial crystallographic directions, desirable doping characteristics and remarkable antireflection property can be readily obtained. The Si nanowire arrays have the potential applicability as an antireflective layer for photovoltaic devices and optical detectors. Furthermore, a combination of this method and the nanosphere lithography has been developed to fabricate large-scale Si and Si1−xGex quantum dot arrays with controllable height, diameter and center-to-center distance.

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Abstract Funding Acknowledgements Type of funding sources: None. Purpose The aim of this study was to investigate native T1 mapping cardiac magnetic resonance (CMR) tomography for the differential diagnosis of left ventricular (LV) hypertrophy. Background Mapping techniques are useful to characterize myocardial tissue abnormalities, particularly cardiac amyloidosis. However, specific cut-off values to differentiate LV hypertrophic phenotypes on 3.0 tesla CMR scanners have not been established, yet. Methods We retrospectively identified patients in the CMR database of Leipzig university hospital with increased LV wall thickness (≥12mm diameter at end-diastole) who were referred for the evaluation of LV hypertrophy or ischemia between 2017 and 2020 on a 3T scanner (Philips Achieva). Patients with suspected or confirmed myocarditis were excluded. Diagnosis of cardiac amyloidosis was made by either biopsy or non-invasively by bone scintigraphy and screening for monoclonal gammopathy. T1 mapping was measured as global mean value from 3 short axis slices of the LV. Results 128 consecutive patients were included in the study. 31 subjects without evidence of structural heart disease served as healthy control. The final diagnosis was cardiac amyloidosis in 24 patients (5 patients with light-chain, 18 with transthyretin amyloidosis, 1 undetermined), hypertrophic cardiomyopathy in 24, and hypertensive heart disease in 80 patients. Mean age of patients was 65 ± 13years, 84% were male. LV mass was increased in patients with LV hypertrophy compared to healthy control (p &lt; 0.001). Native T1 values of the LV myocardium were higher in patients with cardiac amyloidosis (1409 ± 59ms, p &lt; 0.0001 vs. all other groups) compared to healthy control (1225 ± 21ms), patients with hypertrophic cardiomyopathy (HCM; 1263 ± 43ms) and hypertensive heart disease (HHD; 1257 ± 41ms) (Figure). Patients with hypertrophic cardiomyopathy and hypertensive heart disease did not differ in their native T1 values, but both groups were increased compared to healthy control (p &lt; 0.01). Receiver operating characteristic analysis of native T1 values demonstrated an area under the curve for the detection of cardiac amyloidosis of 0.9954 (p &lt; 0.0001) vs. hypertrophic cardiomyopathy, hypertensive heart disease and healthy control. The optimal cut-off value was 1341ms, with a sensitivity of 100% and a specificity of 97%. Conclusion Native T1 mapping has high diagnostic accuracy for the diagnosis of cardiac amyloidosis among patients with LV hypertrophy. These data need confirmation in a prospective clinical trial. Study ID DRKS00022048

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Find your black holes and white walls, know them … it is the only way you will be able to dismantle them and draw your lines of flight.—Deleuze and Guattari, A Thousand Plateaus Defined by originator David Belle as “an art to help you pass any obstacle”, the practice of “parkour” or “free running” constitutes both a mode of movement and a new way of interacting with the urban environment. Parkour was created by Belle (partly in collaboration with his childhood friend Sébastien Foucan) in France in the late 1980s. As seen in the following short video “Rush Hour”, a trailer for BBC One featuring Belle, parkour practitioners (known as “traceurs”), leap, spring, and vault from objects in the urban milieu that are intended to limit movement (walls, curbs, railings, fences) or that unintentionally hamper passage (lampposts, street signs, benches) through the space. “Rush Hour” was among the first media representations of parkour, and it had a significant role in introducing and popularizing the practice in Britain. Parkour has subsequently been widely disseminated via news reports, Nike and Toyota ads, the documentaries Jump London (2003) and Jump Britain (2005), and feature films, including Luc Besson’s Yamakasi – Les Samouraïs des Temps Modernes (2001) and Banlieu 13 (2004; just released in the U.S. as District B13), starring David Belle as Leto and Cyril Raffaelli as Damien. Sébastien Foucan will appear in the upcoming James Bond film Casino Royale as Mollaka, a terrorist who is chased (parkour-style) and then killed by Bond. (Foucan can also be seen in the film’s trailer, currently available at both SonyPictures.com and AOL.com; the film itself is scheduled for release in November 2006). Madonna’s current “Confessions” tour features an extended parkour sequence (accompanying the song “Jump”), albeit one limited to the confines of a scaffold erected over the stage. Perhaps most important in the rapid development of parkour into a world-wide youth movement, however, has been the proliferation of parkour websites featuring amateur videos, photos, tutorials, and blogs. The word “parkour” is derived from the French “parcours” (as the sport is known in France): a line, course, circuit, road, way or route, and the verb “parcourir”: to travel through, to run over or through, to traverse. As a physical discipline, parkour might be said to have a “poetics” — first, in general, in the Aristotelian sense of constructing through its various techniques (tekhnē) the drama of each parkour event. Secondly, one can consider parkour following Aristotle’s model of four-cause analysis as regards its specific materials (the body and the city), form or “vocabulary” of movements (drawn primarily from gymnastics, the martial arts, and modern dance), genre (as against, say, gymnastics), and purpose, including its effects upon its audience and the traceurs themselves. The existing literature on parkour (at this point, mostly news reports or websites) tends to emphasize the elements of form or movement, such as parkour’s various climbs, leaps, vaults, and drops, and the question of genre, particularly the ongoing, heated disputes among traceurs as to what is or is not true parkour. By contrast, my argument in this essay will focus principally on the materials and purpose of parkour: on the nature of the city and the body as they relate to parkour, and on the ways in which parkour can be seen to “remap” urban space and to demonstrate a resistance to its disciplinary functions, particularly as manifest in the urban street “grid.” The institution of the street “grid” (or variations upon it such as Haussmann’s Parisian star-configuration) facilitates both the intelligibility — in terms of both navigation and surveillance — and control of space in the city. It situates people in urban spaces in determinate ways and channels the flow of pedestrian and vehicular traffic. The “grid” thus carries a number of normalizing and disciplinary functions, creating in effect what the philosophers Gilles Deleuze and Félix Guattari refer to as a “striation” of urban space. This striation constitutes “a process of capture of flows of all kinds, populations, commodities or commerce, money or capital, etc.” within a field of determinate spatial coordinates (Deleuze and Guattari 386). It establishes “fixed paths in well-defined directions, which restrict speed, regulate circulation, relativize movement, and measure in detail the relative movements of subjects and objects” (Deleuze and Guattari 386). Many of these aspects of striation can be seen in the ways urban space is depicted in the “Rush Hour” video: in the gridlocked traffic, the flashing tail-lights, the “STOP” light and “WAIT” sign, the sign indicating the proper directional flow of traffic, and the grim, bundled-up pedestrians trudging home en masse along the congested streets. Against these images of conformity, regulation, and confinement, the video presents the parkour ethos of originality, “reach,” escape, and freedom. Belle’s (shirtless) aerial traversal of the urban space between his office and his flat — a swift, improvisational flow across the open rooftops (and the voids between them), off walls, and finally down the sloping roof into his apartment window — cuts across the striated space of the streets below and positions him, for that time, beyond the constrictions of the social realm and its “concrete” manifestations. Though parkour necessarily involves obstacles that must be “overcome,” the goal of parkour is to do this as smoothly and efficiently as possible, or, in the language of its practitioners, for the movement to be “fluid like water.” The experience of parkour might, then, be said to transform the urban landscape into “smooth space,” in Deleuze and Guattari’s sense of “a field without conduits or channels” (371), and thus into a space of uninhibited movement, at least in certain ideal moments. Parkour seems to trace a path of desire (even if the desire is simply to avoid the crowds and get home in time to watch BBC One) that moves along a Deleuzean “line of flight,” a potential avenue of escape from the forces of striation and repression. Here the body is propelled over or through (most parkour movement actually takes place at ground level) the strata of urban space, perhaps with the hope that, as Deleuze and Guattari suggest, “one will bolster oneself directly on a line of flight enabling one to blow apart strata, cut roots, and make new connections” (15). In the process, parkour becomes “an art of displacement,” appropriating urban space in ways that temporarily disrupt their controlling logics and even imply the possibility of a smooth space of desire. One might see parkour as an overcoming of social space (and its various constrictions and inhibitions of desire, its “stop” and “wait” signs) through the interplay of body and material barriers. The body becomes an instrument of freedom. This, again, is graphically conveyed in “Rush Hour” through the opening scene in which Belle strips off his business suit and through the subsequent repeated contrasts of his limber, revealed body to the rigid, swathed figures of the pedestrians below. In part an effect of the various camera angles from which it is shot, there is also an element of the “heroic” in this depiction of the body. This aspect of the representation appears to be knowingly acknowledged in the video’s opening sequence. The first frame is a close-up, tightly focused on a model of a ninja-like figure with a Japanese sword who first appears to be contemplating a building (with an out-of-focus Belle in the background contemplating it from the opposite direction), but then, in the next, full shot, is revealed to be scaling it — in the manner of superheroes and King Kong. The model remains in the frame as Belle undresses (inevitably evoking images of Clark Kent stripping down to his Superman costume) and, in the final shot of that sequence, the figure mirrors Belle’s as he climbs through the window and ascends the building wall outside. In the next sequence, Belle executes a breath-taking handstand on a guard railing on the edge of the roof with the panorama of the city behind him, his upper body spanning the space from the street to the edge of the city skyline, his lower body set against the darkening sky. Through the practice of parkour, the relation between body and space is made dynamic, two reality principles in concert, interacting amid a suspension of the social strata. One might even say that the urban space is re-embodied — its rigid strata effectively “liquified.” In Jump London, the traceur Jerome Ben Aoues speaks of a Zen-like “harmony between you and the obstacle,” an idealization of what is sometimes described as a state of “flow,” a seemingly effortless immersion in an activity with a concomitant loss of self-consciousness. It suggests a different way of knowing the city, a knowledge of experience as opposed to abstract knowledge: parkour is, Jaclyn Law argues, “about curiosity and seeing possibilities — looking at a lamppost or bus shelter as an extension of the sidewalk” (np.). “You just have to look,” Sébastien Foucan insists in Jump London, “you just have to think like children….” Parkour effectively remaps urban space, creating a parallel, “ludic” city, a city of movement and free play within and against the city of obstacles and inhibitions. It reminds us that, in the words of the philosopher of urban space Henri Lefebvre, “the space of play has coexisted and still coexists with spaces of exchange and circulation, political space and cultural space” (172). Parkour tells us that in order to enter this space of play, we only need to make the leap. References Deleuze, Gilles and Félix Guattari. A Thousand Plateaus: Capitalism and Schizophrenia. Trans. Brian Massumi. Minneapolis: University of Minnesota Press, 1987. Jump London (2003). Mike Christie, director. Mike Smith, producer. Featuring Jerome Ben Aoues, Sébastien Foucan, and Johann Vigroux. Law, Jaclyn. “PK and Fly.” This Magazine May/June 2005 http://www.thismagazine.ca/issues/2005/05/>. Lefebvre, Henri. “Perspective or Prospective?” Writings on Cities. Trans. Eleonore Kofman and Elizabeth Lebas. Oxford: Blackwell, 1996. Rush Hour (2002). BBC One promotion trailer. Tom Carty, dir. Edel Erickson, pro. Produced by BBC Broadcast. See also: Wikipedia on parkour: http://www.en.wikipedia.org/wiki/Parkour> Parkour Worldwide Association: http://www.pawa.fr/> Parkour Net (multilingual): http://parkour.net/> NYParkour: http://www.nyparkour.com/> PKLondon.com: http://www.pklondon.com/> Nike’s “The Angry Chicken” (featuring Sébastien Foucan): http://video.google.com/videoplay? docid=-6571575392378784144&q=nike+chicken> There is an extensive collection of parkour videos available at YouTube A rehearsal clip featuring Sébastien Foucan coaching the dancers for Madonna’s Confessions tour can be seen at YouTube Citation reference for this article MLA Style Geyh, Paula. "Urban Free Flow: A Poetics of Parkour." M/C Journal 9.3 (2006). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0607/06-geyh.php>. APA Style Geyh, P. (Jul. 2006) "Urban Free Flow: A Poetics of Parkour," M/C Journal, 9(3). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0607/06-geyh.php>.

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Connections In Small Pieces Loosely Joined, David Weinberger identifies some of the obvious changes which the Web has brought to human relations. Social connections, he argues, used to be exclusively defined and constrained by the physics and physicality of the “real” world, or by geographical and material facts: it’s … true that we generally have to travel longer to get to places that are farther away; that to be heard at the back of the theater, you have to speak louder; that when a couple moves apart, their relationship changes; that if I give you something, I no longer have it. (xi) The Web, however, is a place (or many places) where the boundaries of space, time, and presence are being reworked. Further, since we built this virtual world ourselves and are constantly involved in its evolution, the Web can tell us much about who we are and how we relate to others. In Weinberger’s view, it demonstrates that “we are creatures who care about ourselves and the world we share with others”, and that “we live within a context of meaning” beyond what we had previously cared to imagine (xi-xii). Before the establishment of computer-mediated communication (CMC), we already had multiple means of connecting people commonly separated by space (Gitelman and Pingree). Yet the Web has allowed us to see each other whilst separated by great distances, to share stories, images and other media online, to co-construct or “produse” (Bruns) content and, importantly, to do so within groups, rather than merely between individuals (Weinberger 108). This optimistic evaluation of the Web and social relations is a response to some of the more cautious public voices that have accompanied recent technological developments. In the 1990s, Jan van Dijk raised concerns about what he anticipated as wide-reaching social consequences in the new “age of networks” (2). The network society, as van Dijk described it, was defined by new interconnections (chiefly via the World Wide Web), increased media convergence and narrowcasting, a spread of both social and media networks and the decline of traditional communities and forms of communication. Modern-day communities now consisted both of “organic” (physical) and “virtual” communities, with mediated communication seemingly beginning to replace, or at least supplement, face-to-face interaction (24). Recently, we have found ourselves on the verge of even more “interconnectedness” as the future seems determined by ubiquitous computing (ubicomp) and a new technological and cultural development known as the “Internet of Things” (Greenfield). Ubicomp refers to the integration of information technology into everyday objects and processes, to such an extent that the end-users are often unaware of the technology. According to Greenfield, ubicomp has significant potential to alter not only our relationship with technology, but the very fabric of our existence: A mobile phone … can be switched off or left at home. A computer … can be shut down, unplugged, walked away from. But the technology we're discussing here–ambient, ubiquitous, capable of insinuating itself into all the apertures everyday life affords it–will form our environment in a way neither of those technologies can. (6) Greenfield's ideas are neither hypothesis, nor hyperbole. Ubicomp is already a reality. Dodson notes, Ubicomp isn't just part of our ... future. Its devices and services are already here. Think of the use of prepaid smart cards for use of public transport or the tags displayed in our cars to help regulate congestion charge pricing or the way in which corporations track and move goods around the world. (7) The Internet of Things advances the ubicomp notion of objects embedded with the capacity to receive and transmit data and anticipates a move towards a society in which every device is “on” and in some way connected to the Internet; in other words, objects become networked. Information contained within and transmitted among networked objects becomes a “digital overlay” (Valhouli 2) over the physical world. Valhouli explains that objects, as well as geographical sites, become part of the Internet of Things in two ways. Information may become associated with a specific location using GPS coordinates or a street address. Alternatively, embedding sensors and transmitters into objects enables them to be addressed by Internet protocols, and to sense and react to their environments, as well as communicate with users or with other objects. (2) The Internet of Things is not a theoretical paradigm. It is a framework for describing contemporary technological processes, in which communication moves beyond the established realm of human interaction, to enable a whole range of potential communications: “person-to-device (e.g. scheduling, remote control, or status update), device-to-device, or device-to-grid” (Valhouli 2). Are these newer forms of communication in any sense meaningful? Currently, ubicomp's applications are largely functional, used in transport, security, and stock control. Yet, the possibilities afforded by the technology can be employed to enhance “connectedness” and “togetherness” in the broadest social sense. Most forms of technology have at least some social impact; this is particularly true of communication technology. How can that impact be made explicit? Here, we discuss one such potential application of ubicomp with reference to a new UK research project: TOTeM–Tales of Things and Electronic Memory. TOTeM aims to draw on personal narratives, digital media, and tagging to create an “Internet” of people, things, and object memories via Web 2.0 and mobile technologies. Communicating through Objects The TOTeM project, began in August 2009 and funded by Research Councils UK's Digital Economy Programme, is concerned with eliciting the memory and value of “old” artefacts, which are generally excluded from the discourse of the Internet of Things, which focuses on new and future objects produced with embedded sensors and transmitters. We focus instead on existing artefacts that hold significant personal resonance, not because they are particularly expensive or useful, but because they contain or “evoke” (Turkle) memories of people, places, times, events, or ideas. Objects across a mantelpiece can become conduits between events that happened in the past and people who will occupy the future (Miller 30). TOTeM will draw on user-generated content and innovative tagging technology to study the personal relationships between people and objects, and between people through objects. Our hypothesis is that the stories that are connected to particular objects can become binding ties between individuals, as they provide insights into personal histories and values that are usually not shared, not because they are somehow too personal or uninteresting, but because there is currently little systematic context for sharing them. Even in families, where objects routinely pass down through generations, the stories associated with these objects are generally either reduced to a vague anecdote or lost entirely. Beyond families, there are some objects whose stories are deemed culturally-significant: monuments, the possessions of historical figures, religious artefacts, and archaeological finds. The current value system which defines an object’s cultural significance appears to replicate Bourdieu's assessment of the hierarchies which define aesthetic concepts such as taste. In both cases, the popular, everyday, or otherwise mundane is deemed to possess less cultural capital than that which is less accessible or otherwise associated with the social elites. As a result, objects whose histories are well-known are mostly found in museums, untouchable and unused, whereas objects which are within reach, all around us, tend to travel from owner to owner without anyone considering what histories they might contain. TOTeM’s aim is to provide both a context and a mechanism for enabling individuals and community groups to share object-related stories and memories through digital media, via a custom-built platform of “tales of things”. Participants will be able to use real-life objects as conduits for memory, by producing “tales” about the object's personal significance, told through digital video, photographs, audio, or a mixture of media. These tales will be hosted on the TOTeM project's website. Through specifically-developed TOTeM technology, each object tale will generate a unique physical tag, initially in the form of RFID (Radio Frequency Identification) and QR (Quick Response) codes. TOTeM participants will be able to attach these tags/codes to their objects. When scanned with a mobile phone equipped with free TOTeM software or an RFID tag reader, each tag will access the individual object's tale online, playing the media files telling that object’s story on the mobile phone or computer. The object's user-created tale will be persistently accessible via both the Internet and 3G (third generation) mobile phones. The market share of 3G and 4G mobile networks is expanding, with some analysts predicting that they will account for 30% of the global mobile phone market by 2014 (Kawamoto). As the market for mobile phones with fast data transfer rates keeps growing, TOTeM will become accessible to an ever-growing number of mobile, as well as Internet, users. The TOTeM platform will serve two primary functions. It will become an archive for object memories and thus grow to become an “archaeology for the future”. We hope that future generations will be able to return to this repository and learn about the things that are meaningful to groups and individuals right now. The platform will also serve as an arena for contemporary communication. As the project develops, object memories will be directly accessible through tagged artefacts, as well as through browsing and keyword searches on the project website. Participants will be able to communicate via the TOTeM platform. On a practical level, the platform can bring together people who already share an interest in certain objects, times, or places (e.g. collectors, amateur historians, genealogists, as well as academics). In addition, we hope that the novelty of TOTeM’s approach to objects may encourage some of those individuals for whom non-participation in the digital world is not a question of access but one of apathy and perceived irrelevance (Ofcom 3). Tales of Things: Pilots Since the beginning of this research project, we have begun to construct the TOTeM platform and develop the associated tagging technology. While the TOTeM platform is being built, we have also used this time to conduct a pilot “tale-telling” phase, with the aim of exploring how people might choose to communicate object stories and how this might make them feel. In this initial phase, we focus on eliciting and constructing object tales, without the use of the TOTeM platform or the tagging technology, which will be tested in a future trial. Following Thomson and Holland’s autoethnographic approach, in the first instance, the TOTeM team and advisors shared their own tales with each other (some of these can be viewed on the TOTeM Website). Each of us chose an object that was personally significant to us, digitally recorded our object memories, and uploaded videos to a YouTube channel for discussion amongst the group. Team members in Edinburgh subsequently involved a group of undergraduate students in the pilot. Here, we offer some initial reflections on what we have learned from recording and sharing these early TOTeM tales. The objects the TOTeM team and advisors chose independently from each other included a birth tag, a box of slides, a tile, a block of surf wax, a sweet jar from Japan, a mobile phone, a concert ticket, a wrist band, a cricket bat, a watch, an iPhone, a piece of the Berlin Wall, an antique pocket sundial, and a daughter’s childhood toy. The sheer variety of the objects we selected as being personally significant was intriguing, as were the varying reasons for choosing the objects. Even there was some overlap in object choice, for instance between the mobile and the iPhone, the two items (one (relatively) old, one new) told conspicuously different stories. The mobile held the memory of a lost friend via an old text message; the iPhone was valued not only for its practical uses, but because it symbolised the incarnation of two childhood sci-fi fantasies: a James Bond-inspired tracking device (GPS) and the “Hitchhiker’s Guide to the Galaxy”. While the memories and stories linked to these objects were in many ways idiosyncratic, some patterns have emerged even at this early stage. Stories broadly differed in terms of whether they related to an individual’s personal experience (e.g. memorable moments or times in one’s life) or to their connection with other people. They could also relate to the memory of particular events, from football matches, concerts and festivals on a relatively local basis, to globally significant milestones, such as the fall of the Berlin Wall. In many cases, objects had been kept as tokens and reminders of particularly “colourful” and happy times. One student presented a wooden stick which he had picked up from a beach on his first parent-free “lads’ holiday”. Engraved on the stick were the names of the friends who had accompanied him on this memorable trip. Objects could also mark the beginning or end of a personal life stretch: for one student, his Dub Child vinyl record symbolised the moment he discovered and began to understand experimental music; it also constituted a reminder of the influence his brother had had on his musical taste. At other times, objects were significant because they served as mementos for people who had been “lost” in one way or another, either because they had moved to different places, or because they had gone missing or passed away. With some, there was a sense that the very nature of the object enabled the act of holding on to a memory in a particular way. The aforementioned mobile phone, though usually out of use, was actively recharged for the purposes of remembering. Similarly, an unused wind-up watch was kept going to simultaneously keep alive the memory of its former owner. It is commonly understood that the sharing of insights into one’s personal life provides one way of building and maintaining social relationships (Greene et al.). Self-disclosure, as it is known in psychological terms, carries some negative connotations, such as making oneself vulnerable to the judgement of others or giving away “too much too soon”. Often its achievement is dependent on timing and context. We were surprised by the extent to which some of us chose to disclose quite sensitive information with full knowledge of eventually making these stories public online. At the same time, as both researchers and, in a sense, as an audience, we found it a humbling experience to be allowed into people’s and objects’ meaningful pasts and presents. It is obvious that the invitation to talk about meaningful objects also results in stories about things and people we deeply care about. We have yet to see what shape the TOTeM platform will take as more people share their stories and learn about those of others. We don’t know whether it will be taken up as a fully-fledged communication platform or merely as an archive for object memories, whether people will continue to share what seem like deep insights into personal life stories, or if they choose to make more subversive (no less meaningful) contributions. Likewise, it is yet to be seen how the linking of objects with personal stories through tagging could impact people’s relationships with both the objects and the stories they contain. To us, this initial trial phase, while small in scale, has re-emphasised the potential of sharing object memories in the emerging network of symbolic meaning (Weinberger’s “context of meaning”). Seemingly everyday objects did turn out to contain stories behind them, personal stories which people were willing to share. Returning to Weinberger’s quote with which we began this article, TOTeM will enable the traces of material experiences and relationships to become persistently accessible: giving something away would no longer mean entirely not having it, as the narrative of the object’s significance would persist, and can be added to by future participants. Indeed, TOTeM would enable participants to “give away” more than just the object, while retaining access to the tale which would augment the object. Greenfield ends his discussion of the potential of ubicomp by listing multiple experiences which he does not believe would benefit from any technological augmentation: Going for a long run in the warm gentle rain, gratefully and carefully easing my body into the swelter of a hot springs, listening to the first snowfall of winter, savouring the texture of my wife’s lips … these are all things that require little or no added value by virtue of being networked, relational, correlated to my other activities. They’re already perfect, just as they stand. (258) It is a resonant set of images, and most people would be able to produce a similar list of meaningful personal experiences. Yet, as we have already suggested, technology and meaning need not be mutually exclusive. Indeed, as the discussion of TOTeM begins to illustrate, the use of new technologies in new contexts can augment the commercial applications of ubiquoutous computing with meaningful human communication. At the time of writing, the TOTeM platform is in the later stages of development. We envisage the website taking shape and its content becoming more and more meaningful over time. However, some initial object memories should be available from April 2010, and the TOTeM platform and mobile tagging applications will be fully operational in the summer of 2010. Our progress can be followed on www.youtotem.com and http://twitter.com/talesofthings. TOTeM looks forward to receiving “tales of things” from across the world. References Bourdieu, Pierre. Distinction: A Social Critique of the Judgement of Taste. London: Routledge, 1984.Bruns, Axel. “The Future is User-Led: The Path towards Widespread Produsage.” fibreculture 11 (2008). 20 Mar. 2010 ‹http://www.journal.fibreculture.org/issue11/issue11_bruns_print.html›. Dodson, Sean. “Forward: A Tale of Two Cities.” Rob van Kranenburg. The Internet of Things: A Critique of Ambient Technology and the All-Seeing Network of RFID. Amsterdam: Institute of Network Cultures, Network Notebooks 02, 2008. 5-9. 20 Mar. 2010 ‹http://www.networkcultures.org/_uploads/notebook2_theinternetofthings.pdf›. Gitelman, Lisa, and Geoffrey B. Pingree. Eds. New Media: 1740-1915. Cambridge, MA: MIT Press, 2003. Greene, Kathryn, Valerian Derlega, and Alicia Mathews. “Self-Disclosure in Personal Relationships.” Ed. Anita L. Vangelisti and Daniel Perlman. Cambridge Handbook of Personal Relationships. Cambridge: Cambridge UP, 2006. 409-28. Greenfield, Adam. Everyware: The Dawning Age of Ubiquitous Computing. Berkeley, CA: New Riders, 2006. Kawamoto, Dawn. “Report: 3G and 4G Market Share on the Rise.” CNET News 2009. 20 Mar. 2010 ‹http://news.cnet.com/8301-1035_3-10199185-94.html›. Kwint, Marius, Christopher Breward, and Jeremy Aynsley. Material Memories: Design and Evocation. Oxford: Berg, 1999. Miller, Daniel. The Comfort of Things. Cambridge: Polity Press, 2008. Ofcom. ”Accessing the Internet at Home”. 2009. 20 Mar. 2010 ‹http://www.ofcom.org.uk/research/telecoms/reports/bbresearch/bbathome.pdf›. Thomson, Rachel, and Janet Holland. “‘Thanks for the Memory’: Memory Books as a Methodological Resource in Biographical Research.” Qualitative Research 5.2 (2005): 201-19. Turkle, Sherry. Evocative Objects: Things We Think With. Cambridge, MA: MIT Press, 2007. Valhouli, Constantine A. The Internet of Things: Networked Objects and Smart Devices. The Hammersmith Group Research Report, 2010. 20 Mar. 2010 ‹http://thehammersmithgroup.com/images/reports/networked_objects.pdf›. Van Dijk, Jan. The Network Society: Social Aspects of New Media. London: SAGE, 1999. Weinberger, David. Small Pieces Loosely Joined: How the Web Shows Us Who We Really Are. Oxford: Perseus Press, 2002.

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IntroductionThis short article examines contemporary artistic use of walkie-talkies across two projects: Saturday (2002) by Sabrina Raaf and Walk That Sound (2014) by Lukatoyboy. Drawing on Dominic Pettman’s notion of sonic intimacy, I argue that both artists incorporate walkie-talkies as part of their explorations of mediated wandering, and in ways that seek to capture sonic ambiances and intimacies. One thing that is striking about both these works is that they rethink what’s possible with walkie-talkies; both artists use them not just as low-tech, portable devices for one-to-one communication over distance, but also—and more strikingly—as (covert) recording equipment for capturing, while wandering, snippets of intimate conversation between passers-by and the “voice” of the surrounding environment. Both artworks strive to make the familiar strange. They prompt us to question our preconceived perceptions of, and affective engagements with, the people and places around us, to listen more attentively to the voices of others (and the “Other”), and to aurally inhabit in new ways the spaces and places we find ourselves in and routinely pass through.The walkie-talkie is an established, simple communication device, consisting of a two-way radio transceiver with a speaker and microphone (in some cases, the speaker is also used as the microphone) and an antenna (Wikipedia). Walkie-talkies are half-duplex communication devices, meaning that they use a single radio channel: only one radio on the channel can transmit at a time, but many can listen; when a user wishes to talk, they must turn off the receiver and turn on the transmitter by pressing a push-to-talk button (Wikipedia). In some models, static—known as squelch—is produced each time the push-to-talk button is depressed. The push-to-talk button is a feature of both projects: in Saturday, it transforms the walkie-talkie into a cheap, portable recorder-transmitter. In Walk That Sound, rapid fire exchanges of conversation using the push-to-talk button feature strongly.Interestingly, walkie-talkies were developed during World War Two. While they continue to be used within certain industrial settings, they are perhaps best known as a “quaint” household toy and “fun tool” (Smith). Early print ads for walkie-talkie toys marketed them as a form of both spyware for kids (with the Gabriel Toy Co. releasing a 007-themed walkie-talkie set) and as a teletechnology for communication over distance—“how thrilling to ‘speak through space!’”, states one ad (Statuv “New!”). What is noteworthy about these early ads is that they actively promote experimental use of walkie-talkies. For instance, a 1953 ad for Vibro-Matic “Space Commander” walkie-talkies casts them as media transmission devices, suggesting that, with them, one can send and receive “voice – songs – music” (Statuv “New!”). In addition, a 1962 ad for the Knight-Kit walkie-talkie imagines “you’ll find new uses for this exciting walkie-talkie every day” (Statuv “Details”). Resurgent interest in walkie-talkies has seen them also promoted more recently as intimate tools “for communication without asking permission to communicate” (“Nextel”); this is to say that they have been marketed as devices for synchronous or immediate communication that overcome the limits of asynchronous communication, such as texting, where there might be substantial delays between the sending of a message and receipt of a response. Within this context, it is not surprising that Snapchat and Instagram have also since added “walkie-talkie” features to their messaging services. The Nextel byline, emphasising “without asking permission”, also speaks to the possibilities of using walkie-talkies as rudimentary forms of spyware.Within art practice that explores mediated forms of wandering—that is, walking while using media and various “remote transmission technologies” (Duclos 233)—walkie-talkies hold appeal for a number of reasons, including their particular aesthetic qualities, such as the crackling or static sound (squelch) that one encounters when using them; their portability; their affordability; and, the fact that, while they can be operated on multiple channels, they tend to be regarded primarily as devices that permit two-way, one-to-one (and therefore intimate, if not secure) remote communication. As we will see below, however, contemporary artists, such as the aforementioned earlier advertisers, have also been very attentive to the device’s experimental possibilities. Perhaps the best known (if possibly apocryphal) example of artistic use of walkie-talkies is by the Situationist International as part of their explorations in urban wandering (a revolutionary strategy called dérive). In the Situationist text from 1960, Die Welt als Labyrinth (Anon.), there is a detailed account of how walkie-talkies were to form part of a planned dérive, which was organised by the Dutch section of the Situationist International, through the city of Amsterdam, but which never went ahead:Two groups, each containing three situationists, would dérive for three days, on foot or eventually by boat (sleeping in hotels along the way) without leaving the center of Amsterdam. By means of the walkie-talkies with which they would be equipped, these groups would remain in contact, with each other, if possible, and in any case with the radio-truck of the cartographic team, from where the director of the dérive—in this case Constant [Nieuwenhuys]—moving around so as to maintain contact, would define their routes and sometimes give instructions (it was also the director of the dérive’s responsibility to prepare experiments at certain locations and secretly arranged events.) (Anon.) This proposed dérive formed part of Situationist experiments in unitary urbanism, a process that consisted of “making different parts of the city communicate with one another.” Their ambition was to create new situations informed by, among other things, encounters and atmospheres that were registered through dérive in order to reconnect parts of the city that were separated spatially (Lefebvre quoted in Lefebvre and Ross 73). In an interview with Kristin Ross, Henri Lefebvre insists that the Situationists “did have their experiments; I didn’t participate. They used all kinds of means of communication—I don’t know when exactly they were using walkie-talkies. But I know they were used in Amsterdam and in Strasbourg” (Lefebvre quoted in Lefebvre and Ross 73). However, as Rebecca Duclos points out, such use “is, in fact, not well documented”, and “none of the more well-known reports on situationist activity […] specifically mentions the use of walkie-talkies within their descriptive narratives” (Duclos 233). In the early 2000s, walkie-talkies also figured prominently, alongside other media devices, in at least two location-based gaming projects by renowned British art collective Blast Theory, Can You See Me Now? (2001) and You Get Me (2008). In the first of these projects, participants in the game (“online players”) competed against members of Blast Theory (“runners”), tracking them through city streets via a GPS-enabled handheld computer that runners carried with them. The goal for online players was to move an avatar they created through a virtual map of the city as multiple runners “pursued their avatar’s geographical coordinates in real-time” (Leorke). As Dale Leorke explains, “Players could see the locations of the runners and other players and exchange text messages with other players” (Leorke 27), and runners could “read players’ messages and communicate directly with each other through a walkie-talkie” (28). An audio stream from these walkie-talkie conversations allowed players to eavesdrop on their pursuers (Blast Theory, Can You See Me Now?).You Get Me was similarly structured, with online players and “runners” (eight teenagers who worked with Blast Theory on the game). Remotely situated online players began the game by listening to the “personal geography” of the runners over a walkie-talkie stream (Blast Theory, You Get Me). They then selected one runner, and tracked them down by navigating their own avatar, without being caught, through a virtual version of Mile End Park in London, in pursuit of their chosen runner who was moving about the actual Mile End Park. Once their chosen runner was contacted, the player had to respond to a question that the runner posed to them. If the runner was satisfied with the player’s answer, conversation switched to “the privacy of a mobile phone” in order to converse further; if not, the player was thrown back into the game (Blast Theory, You Get Me). A key aim of Blast Theory’s work, as I have argued elsewhere (Wilken), is the fostering of interactions and fleeting intimacies between relative and complete strangers. The walkie-talkie is a key tool in both the aforementioned Blast Theory projects for facilitating these interactions and intimacies.Beyond these well-known examples, walkie-talkies have been employed in productive and exploratory ways by other artists. The focus in this article is on two specific projects: the first by US-based sound artist Sabrina Raaf, called Saturday (2002) and the second by Serbian sound designer Lukatoyboy (Luka Ivanović), titled Walk That Sound (2014). Sonic IntimaciesThe concept that gives shape and direction to the analysis of the art projects by Raaf and Lukatoyboy and their use of walkie-talkies is that of sonic intimacy. This is a concept of emerging critical interest across media and sound studies and geography (see, for example, James; Pettman; Gallagher and Prior). Sonic intimacy, as Dominic Pettman explains, is composed of two simultaneous yet opposing orientations. On the one hand, sonic intimacy involves a “turning inward, away from the wider world, to more private and personal experiences and relationships” (79). While, on the other hand, it also involves a turning outward, to seek and heed “the voice of the world” (79)—or what Pettman refers to as the “vox mundi” (66). Pettman conceives of the “vox mundi” as an “ecological voice”, whereby “all manner of creatures, agents, entities, objects, and phenomena” (79) have the opportunity to speak to us, if only we were prepared to listen to our surroundings in new and different ways. In a later passage, he also refers to the “vox mundi” as a “carrier or potentially enlightening alterity” (83). Voices, Pettman writes, “transgress the neat divisions we make between ‘us’ and ‘them’, at all scales and junctures” (6). Thus, Pettman’s suggestion is that “by listening to the ‘voices’ that lie dormant in the surrounding world […] we may in turn foster a more sustainable relationship with [the] local matrix of specific existences” (85), be they human or otherwise.This formulation of sonic intimacy provides a productive conceptual frame for thinking through Raaf’s and Lukatoyboy’s use of walkie-talkies. The contention in this article is that these two projects are striking for the way that they both use walkie-talkies to explore, simultaneously, this double articulation or dual orientation of sonic intimacy—a turning inwards to capture more private and personal experiences and conversations, and a turning outwards to capture the vox mundi. Employing Pettman’s notion of sonic intimacy as a conceptual frame, I trace below the different ways that these two projects incorporate walkie-talkies in order to develop mediated forms of wandering that seek to capture place-based sonic ambiances and sonic intimacies.Sabrina Raaf, Saturday (2002)US sound artist Sabrina Raaf’s Saturday (2002) is a sound-based art installation based on recordings of “stolen conversations” that Raaf gathered over many Saturdays in Humboldt Park, Chicago. Raaf’s work harks back to the early marketing of walkie-talkie toys as spyware. In Raaf’s hands, this device is used not for engaging in intimate one-to-one conversation, but for listening in on, and capturing, the intimate conversations of others. In other words, she uses this device, as the Nextel slogan goes, for “communication without permission to communicate” (“Nextel”). Raaf’s inspiration for the piece was twofold. First, she has noted that “with the overuse of radio frequency bands for wireless communications, there comes the increased occurrence of crossed lines where a private conversation becomes accidentally shared” (Raaf). Reminiscent of Francis Ford Coppola’s film The Conversation (1974), in which surveillance expert Harry Caul (Gene Hackman) records the conversation of a couple as they walk through crowded Union Square in San Francisco, Raaf used a combination of walkie-talkies, CB radios, and “various other forms of consumer spy […] technology in order to actively harvest such communication leaks” (Raaf). The second source of inspiration was noticing the “sheer quantity of non-phone, low tech, radio transmissions that were constantly being sent around [the] neighbourhood”, transmissions that were easily intercepted. These conversations were eclectic in composition and character:The transmissions included communications between gang members on street corners nearby and group conversations between friends talking about changes in the neighbourhood and their families. There were raw, intimate conversations and often even late night sex talk between potential lovers. (Raaf)What struck Raaf about these conversations, these transmissions, was that there was “a furtive quality” to most of them, and “a particular daringness to their tone”.During her Saturday wanderings, Raaf complemented her recordings of stolen snippets of conversation with recordings of the “voice” of the surrounding neighbourhood—“the women singing out their windows to their radios, the young men in their low rider cars circling the block, the children, the ice cream carts, etc. These are the sounds that are mixed into the piece” (Raaf).Audience engagement with Saturday involves a kind of austere intimacy of its own that seems befitting of a surveillance-inspired sonic portrait of urban and private life. The piece is accessed via an interactive glove. This glove is white in colour and about the size of a large gardening glove, with a Velcro strap that fastens across the hand, like a cycling glove. The glove, which only has coverings for thumb and first two fingers (it is missing the ring and little fingers) is wired into and rests on top of a roughly A4-sized white rectangular box. This box, which is mounted onto the wall of an all-white gallery space at the short end, serves as a small shelf. The displayed glove is illuminated by a discrete, bent-arm desk lamp, that protrudes from the shelf near the gallery wall. Above the shelf are a series of wall-mounted colour images that relate to the project. In order to hear the soundtrack of Saturday, gallery visitors approach the shelf, put on the glove, and “magically just press their fingertips to their forehead [to] hear the sound without the use of their ears” (Raaf). The glove, Raaf explains, “is outfitted with leading edge audio electronic devices called ‘bone transducers’ […]. These transducers transmit sound in a very unusual fashion. They translate sound into vibration patterns which resonate through bone” (Raaf).Employing this technique, Raaf explains, “permits a new way of listening”:The user places their fingers to their forehead—in a gesture akin to Rodin’s The Thinker or of a clairvoyant—in order to tap into the lives of strangers. Pressing different combinations of fingers to the temple yield plural viewpoints and group conversations. These sounds are literally mixed in the bones of the listener. (Raaf) The result is a (literally and figuratively) touching sonic portrait of Humboldt Park, its residents, and the “voice” of its surrounding neighbourhoods. Through the unique technosomatic (Richardson) apparatus—combinations of gestures that convey the soundscape directly through the bones and body—those engaging with Saturday get to hear voices in/of/around Humboldt Park. It is a portrait that combines sonic intimacy in the two forms described earlier in this article. In its inward-focused form, the gallery visitor-listener is positioned as a voyeur of sorts, listening into stolen snippets of private and personal relationships, experiences, and interactions. And, in its outward-focused form, the gallery visitor-listener encounters a soundscape in which an array of agents, entities, and objects are also given a voice. Additional work performed by this piece, it seems to me, is to be found in the intermingling of these two form of sonic intimacy—the personal and the environmental—and the way that they prompt reflection on mediation, place, urban life, others, and intimacy. That is to say that, beyond its particular sonic portrait of Humboldt Park, Saturday works in “clearing some conceptual space” in the mind of the departing gallery visitor such that they might “listen for, if not precisely to, the collective, polyphonic ‘voice of the world’” (Pettman 6) as they go about their day-to-day lives.Lukatoyboy, Walk That Sound (2014)The second project, Walk That Sound, by Serbian sound artist Lukatoyboy was completed for the 2014 CTM festival. CTM is an annual festival event that is staged in Berlin and dedicated to “adventurous music and art” (CTM Festival, “About”). A key project within the festival is CTM Radio Lab. The Lab supports works, commissioned by CTM Festival and Deutschlandradio Kultur – Hörspiel/Klangkunst (among other partnering organisations), that seek to pair and explore the “specific artistic possibilities of radio with the potentials of live performance or installation” (CTM Festival, “Projects”). Lukatoyboy’s Walk That Sound was one of two commissioned pieces for the 2014 CTM Radio Lab. The project used the “commonplace yet often forgotten walkie-talkie” (CTM Festival, “Projects”) to create a moving urban sound portrait in the area around the Kottbusser Tor U-Bahn station in Berlin-Kreuzberg. Walk That Sound recruited participants—“mobile scouts”—to rove around the Kottbusser Tor area (CTM Festival, “Projects”). Armed with walkie-talkies, and playing with “the array of available and free frequencies, and the almost unlimited amount of users that can interact over these different channels”, the project captured the dispatches via walkie-talkie of each participant (CTM Festival, “Projects”). The resultant recording of Walk That Sound—which was aired on Deutschlandradio (see Lukatoyboy), part of a long tradition of transmitting experimental music and sound art on German radio (Cory)—forms an eclectic soundscape.The work juxtaposes snippets of dialogue shared between the mobile scouts, overheard mobile phone conversations, and moments of relative quietude, where the subdued soundtrack is formed by the ambient sounds—the “voice”—of the Kottbusser Tor area. This voice includes distant traffic, the distinctive auditory ticking of pedestrian lights, and moments of tumult and agitation, such as the sounds of construction work, car horns, emergency services vehicle sirens, a bottle bouncing on the pavement, and various other repetitive yet difficult to identify industrial sounds. This voice trails off towards the end of the recording into extended walkie-talkie produced static or squelch. The topics covered within the “crackling dialogues” (CTM Festival, “Projects”) of the mobile scouts ranged widely. There were banal observations (“I just stepped on a used tissue”; “people are crossing the street”; “there are 150 trains”)—wonderings that bear strong similarities with French writer Georges Perec’s well-known experimental descriptions of everyday Parisian life in the 1970s (Perec “An Attempt”). There were also intimate, confiding, flirtatious remarks (“Do you want to come to Turkey with me?”), as well as a number of playfully paranoid observations and quips (“I like to lie”; “I can see you”; “do you feel like you are being recorded?”; “I’m being followed”) that seem to speak to the fraught history of Berlin in particular as well as the complicated character of urban life in general—as Pettman asks, “what does ‘together’ signify in a socioeconomic system so efficient in producing alienation and isolation?” (92).In sum, Walk That Sound is a strangely moving exploration of sonic intimacy, one that shifts between many different registers and points of focus—much like urban wandering itself. As a work, it is variously funny, smart, paranoid, intimate, expansive, difficult to decipher, and, at times, even difficult to listen to. Pettman argues that, “thanks in large part to the industrialization of the human ear […], we have lost the capacity to hear the vox mundi, which is […] the sum total of cacophonous, heterogeneous, incommensurate, and unsynthesizable sounds of the postnatural world” (8). Walk That Sound functions almost like a response to this dilemma. One comes away from listening to it with a heightened awareness of, appreciation for, and aural connection to the rich messiness of the polyphonic contemporary urban vox mundi. ConclusionThe argument of this article is that Sabrina Raaf’s Saturday and Lukatoyboy’s Walk That Sound are two projects that both incorporate walkie-talkies in order to develop mediated forms of wandering that seek to capture place-based sonic ambiances and sonic intimacies. Drawing on Pettman’s notion of “sonic intimacy”, examination of these projects has opened consideration around voice, analogue technology, and what Nick Couldry refers to as “an obligation to listen” (Couldry 580). In order to be heard, Pettman remarks, and “in order to be considered a voice at all”, and therefore as “something worth heeding”, the vox mundi “must arrive intimately, or else it is experienced as noise or static” (Pettman 83). In both the projects discussed here—Saturday and Walk That Sound—the walkie-talkie provides this means of “intimate arrival”. As half-duplex communication devices, walkie-talkies have always fulfilled a double function: communicating and listening. This dual functionality is exploited in new ways by Raaf and Lukatoyboy. In their projects, both artists turn the microphone outwards, such that the walkie-talkie becomes not just a device for communicating while in the field, but also—and more strikingly—it becomes a field recording device. The result of which is that this simple, “playful” communication device is utilised in these two projects in two ways: on the one hand, as a “carrier of potentially enlightening alterity” (Pettman 83), a means of encouraging “potential encounters” (89) with strangers who have been thrown together and who cross paths, and, on the other hand, as a means of fostering “an environmental awareness” (89) of the world around us. In developing these prompts, Raaf and Lukatoyboy build potential bridges between Pettman’s work on sonic intimacy, their own work, and the work of other experimental artists. For instance, in relation to potential encounters, there are clear points of connection with Blast Theory, a group who, as noted earlier, have utilised walkie-talkies and sound-based and other media technologies to explore issues around urban encounters with strangers that promote reflection on ideas and experiences of otherness and difference (see Wilken)—issues that are also implicit in the two works examined. In relation to environmental awareness, their work—as well as Pettman’s calls for greater sonic intimacy—brings renewed urgency to Georges Perec’s encouragement to “question the habitual” and to account for, and listen carefully to, “the common, the ordinary, the infraordinary, the background noise” (Perec “Approaches” 210).Walkie-talkies, for Raaf and Lukatoyboy, when reimagined as field recording devices as much as remote transmission technologies, thus “allow new forms of listening, which in turn afford new forms of being together” (Pettman 92), new forms of being in the world, and new forms of sonic intimacy. Both these artworks engage with, and explore, what’s at stake in a politics and ethics of listening. Pettman prompts us, as urban dweller-wanderers, to think about how we might “attend to the act of listening itself, rather than to a specific sound” (Pettman 1). His questioning, as this article has explored, is answered by the works from Raaf and Lukatoyboy in effective style and technique, setting up opportunities for aural attentiveness and experiential learning. However, it is up to us whether we are prepared to listen carefully and to open ourselves to such intimate sonic contact with others and with the environments in which we live.ReferencesAnon. “Die Welt als Labyrinth.” Internationale Situationiste 4 (Jan. 1960). International Situationist Online, 19 June 2019 <https://www.cddc.vt.edu/sionline/si/diewelt.html>Blast Theory. “Can You See Me Now?” Blast Theory, 19 June 2019 <https://www.blasttheory.co.uk/projects/can-you-see-me-now/>.———. “You Get Me.” Blast Theory, 19 June 2019 <https://wwww.blasttheory.co.uk/projects/you-get-me/>.Cory, Mark E. “Soundplay: The Polyphonous Tradition of German Radio Art.” Wireless Imagination: Sound, Radio, and the Avant-garde. Eds. Douglas Kahn and Gregory Whitehead. Cambridge, MA: MIT P, 1992. 331–371.Couldry, Nick. “Rethinking the Politics of Voice.” Continuum 23.4 (2009): 579–582.CTM Festival. “About.” CTM Festival, 2019. 19 June 2019 <https://www.ctm-festival.de/about/ctm-festival/>.———. “Projects – CTM Radio Lab.” CTM Festival, 2019. 19 June 2019 <https://www.ctm-festival.de/projects/ctm-radio-lab/>.Duclos, Rebecca. “Reconnaissance/Méconnaissance: The Work of Janet Cardiff and George Bures Miller.” Articulate Objects: Voice, Sculpture and Performance. Eds. Aura Satz and Jon Wood. Bern: Peter Lang, 2009. 221–246. Gallagher, Michael, and Jonathan Prior. “Sonic Geographies: Exploring Phonographic Methods.” Progress in Human Geography 38.2 (2014): 267–284.James, Malcom. Sonic Intimacy: The Study of Sound. London: Bloomsbury, forthcoming.Lefebvre, Henri, and Kristin Ross. “Lefebvre on the Situationists: An Interview.” October 79 (Winter 1997): 69–83. Leorke, Dale. Location-Based Gaming: Play in Public Space. Singapore: Palgrave Macmillan, 2019.Lukatoyboy. “Walk That Sound – Deutschlandradiokultur Klangkunst Broadcast 14.02.2014.” SoundCloud. 19 June 2019 <https://soundcloud.com/lukatoyboy/walk-that-sound-deutschlandradiokultur-broadcast-14022014>.“Nextel: Couple. Walkie Talkies Are Good for Something More.” AdAge. 6 June 2012. 18 July 2019 <https://adage.com/creativity/work/couple/27993>.Perec, Georges. An Attempt at Exhausting a Place in Paris. Trans. Marc Lowenthal. Cambridge, MA: Wakefield Press, 2010.———. “Approaches to What?” Species of Spaces and Other Pieces. Rev. ed. Ed. and trans. John Sturrock. Harmondsworth, Middlesex: Penguin, 1999. 209–211.Pettman, Dominic. Sonic Intimacy: Voice, Species, Technics (Or, How to Listen to the World). Stanford, CA: Stanford UP, 2017.Raaf, Sabrina. “Saturday.” Sabrina Raaf :: New Media Artist, 2002. 19 June 2019 <http://raaf.org/projects.php?pcat=2&proj=10>.Richardson, Ingrid. “Mobile Technosoma: Some Phenomenological Reflections on Itinerant Media Devices.” The Fibreculture Journal 6 (2005). <http://six.fibreculturejournal.org/fcj-032-mobile-technosoma-some-phenomenological-reflections-on-itinerant-media-devices/>. Smith, Ernie. “Roger That: A Short History of the Walkie Talkie.” Vice, 23 Sep. 2017. 19 June 2019 <https://www.vice.com/en_us/article/vb7vk4/roger-that-a-short-history-of-the-walkie-talkie>. Statuv. “Details about Allied Radio Knight-Kit C-100 Walkie Talkie CB Radio Vtg Print Ad.” Statuv, 4 Jan. 2016. 18 July 2019 <https://statuv.com/media/74802043788985511>.———. “New! 1953 ‘Space Commander’ Vibro-Matic Walkie-Talkies.” Statuv, 4 Jan. 2016. 18 July 2019 <https://statuv.com/media/74802043788985539>.Wikipedia. “Walkie-Talkie”. Wikipedia, 3 July 2019. 18 July 2019 <https://en.wikipedia.org/wiki/Walkie-talkie>.Wilken, Rowan. “Proximity and Alienation: Narratives of City, Self, and Other in the Locative Games of Blast Theory.” The Mobile Story: Narrative Practices with Locative Technologies. Ed. Jason Farman. New York: Routledge, 2014. 175–191.

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Abstract:

IntroductionThe death of Chris Cornell in the spring of 2017 shook me. As the lead singer of Soundgarden and a pioneer of early 1990s grunge music, his voice revealed an unbridled pain and joy backed up by the raw, guitar-driven rock emanating from the Seattle, Washington music scene. I remember thinking, there’s only one left, referring to Eddie Vedder, lead singer for Pearl Jam, and lone survivor of the four seminal grunge bands that rose to fame in the early 1990s whose lead singers passed away much too soon. Alice in Chains singer Layne Staley died in 2002 at the age of 35, and Nirvana front man Kurt Cobain’s death in 1994 had resonated around the globe. I thought about when Cornell and Staley said goodbye to their friend Andy Wood, lead singer of Mother Love Bone, after he overdosed on heroine in 1990. Wood’s untimely death at the age of 24, only days before his band’s debut album release, shook the close-knit Seattle music scene and remained a source of angst and inspiration for a genre of music that shaped youth culture of the 1990s.When grunge first exploded on the pop culture scene, I was a college student flailing around in pursuit of an English degree I had less passion for than I did for music. I grew up listening to The Beatles and Prince; Led Zeppelin and Miles Davis; David Bowie and Willie Nelson, along with a litany of other artists and musicians crafting the kind of meaningful music I responded to. I didn’t just listen to music, I devoured stories about the musicians, their often hedonistic lifestyles; their processes and epiphanies. The music spoke to my being in the world more than the promise of any college degree. I ran with friends who shared this love of music, often turning me on to new bands or suggesting some obscure song from the past to track down. I picked up my first guitar when John Lennon died on the eve of my eleventh birthday and have played for the past 37 years. I rely on music to relocate my sense of self. Rhythm and melody play out like characters in my life, colluding to make me feel something apart from the mundane, moving me from within. So, when I took notice of grunge music in the fall of 1991, it was love at first listen. As a pop cultural phenomenon, grunge ruptured the music and fashion industries caught off guard by its sudden commercial appeal while the media struggled to galvanize its relevance. As a subculture, grunge rallied around a set of attitudes and values that set the movement apart from mainstream (Latysheva). The grunge sound drew from the nihilism of punk and the head banging gospel of heavy metal, tinged with the swagger of 1970s FM rock running counter to the sleek production of pop radio and hair metal bands. Grunge artists wrote emotionally-laden songs that spoke to a particular generation of youth who identified with lyrics about isolation, anger, and death. Grunge set off new fashion trends in favor of dressing down and sporting the latest in second-hand, thrift store apparel, ripping away the Reagan-era starched white-collared working-class aesthetic of the 1980’s corporate culture. Like their punk forbearers who railed against the status quo and the trappings of success incurred through the mass appeal of their art, Kurt Cobain, Eddie Vedder, and the rest of the grunge cohort often wrestled with the momentum of their success. Fortunes rained down and the media ordained them rock stars.This auto-ethnography revisits some of the cultural impacts of grunge during its rise to cultural relevance and includes my own reflexive interpretation positioned as a fan of grunge music. I use a particular auto-ethnographic orientation called “interpretive-humanistic autoethnography” (Manning and Adams 192) where, along with archival research (i.e. media articles and journal articles), I will use my own reflexive voice to interpret and describe my personal experiences as a fan of grunge music during its peak of popularity from 1991 up to the death of Cobain in 1994. It is a methodology that works to bridge the personal and popular where “the individual story leaves traces of at least one path through a shifting, transforming, and disappearing cultural landscape” (Neumann 183). Grunge RootsThere are many conflicting stories as to when the word “grunge” was first used to describe the sound of a particular style of alternative music seeping from the dank basem*nts and shoddy rehearsal spaces in towns like Olympia, Aberdeen, and Seattle. Lester Bangs, the preeminent cultural writer and critic of all things punk, pop, and rock in the 1970s was said to have used the word at one time (Yarm), and several musicians lay claim to their use of the word in the 1980s. But it was a small Seattle record label founded in 1988 called Sub Pop Records that first included grunge in their marketing materials to describe “the grittiness of the music and the energy” (Yarm 195).This particular sound grew out of the Pacific Northwest blue-collar environment of logging towns, coastal fisheries, and airplane manufacturing. Seattle’s alternative music scene unfolded as a community of musicians responding to the tucked away isolation of their musty surroundings, apart from the outside world, free to submerge themselves in their own cultural milieu of rock music, rain, and youthful rebellion.Where Seattle stood as a major metropolitan city soaked in rainclouds for much of the year, I was soaking up the desert sun in a rural college town when grunge first leapt into the mainstream. Cattle ranches and cotton fields spread across the open plains of West Texas, painted with pickup trucks, starched Wrangler Jeans, and cowboy hats. This was not my world. I’d arrived the year prior from Houston, Texas, an urban sprawl of four million people, but I found the wide-open landscape a welcome change from the concrete jungle of the big city. Along with cowboy boots and western shirts came country music, and lots of it. Garth Brooks, Reba McEntire, George Straight; some of the voices that captured the lifestyle of my small rural town, twangy guitars and fiddles blaring on local radio. While popular country artists recorded for behemoth record labels like Warner Brothers and Sony, the tiny Sub Pop Records championed the grunge sound coming out of the Seattle music scene. Sub Pop became a playground for those who cared about their music and little else. The label cultivated an early following through their Sub Pop Singles Club, mailing seven-inch records to subscribers on a monthly basis promoting new releases from up-and-coming bands. Sub Pop’s stark, black and white logo showed up on records sleeves, posters, and t-shirts, reflecting a no-nonsense DIY-attitude rooted in in the production of loud guitars and heavy drums.Like the bands it represented, Sub Pop did not take itself too seriously when one of their best-selling t-shirts simply read “Loser” embracing the slacker mood of newly minted Generation X’ers born between 1961 and 1981. A July 1990 Time Magazine article described this twenty-something demographic as having “few heroes, no anthems, no style to call their own” suggesting they “possess only a hazy sense of their own identity” (Gross & Scott). As a member of this generation, I purchased and wore my “Loser” t-shirt with pride, especially in ironic response to the local cowboy way of life. I didn’t hold anything personal against the Wrangler wearing Garth Brooks fan but as a twenty-one-year-old reluctant college student, I wanted to rage with contempt for the status quo of my environment with an ambivalent snarl.Grunge in the MainstreamIn 1991, the Seattle sound exploded onto the international music scene with the release of four seminal grunge-era albums over a six-month period. The first arrived in April, Temple of the Dog, a tribute album of sorts to the late Andy Wood, led by his close friend, Soundgarden singer/songwriter, Chris Cornell. In August, Pearl Jam released their debut album, Ten, with its “surprising and refreshing, melodic restraint” (Fricke). The following month, Nirvana’s Nevermind landed in stores. Now on a major record label, DGC Records, the band had arrived “at the crossroads—scrappy garageland warriors setting their sights on a land of giants” (Robbins). October saw the release of Soundgarden’s Badmotorfinger as “a runaway train ride of stammering guitar and psycho-jungle telegraph rhythms” (Fricke). These four albums sent grunge culture into the ether with a wall of sound that would upend the music charts and galvanize a depressed concert ticket market.In fall of 1991, grunge landed like a hammer when I witnessed Nirvana’s video for “Smells Like Teen Spirit” on MTV for the first time. Sonically, the song rang like an anthem for the Gen Xers with its jangly four-chord opening guitar riff signaling the arrival of a youth-oriented call to arms, “here we are now, entertain us” (Nirvana). It was the visual power of seeing a skinny white kid with stringy hair wearing baggy jeans, a striped T-shirt and tennis shoes belting out choruses with a ferociousness typically reserved for black-clad heavy metal headbangers. Cobain’s sound and look didn’t match up. I felt discombobulated, turned sideways, as if vertigo had taken hold and I couldn’t right myself. Stopped in the middle of my tracks on that day, frozen in front of the TV, the subculture of grunge music slammed into my world while I was on my way to the fridge.Suddenly, grunge was everywhere, As Soundgarden, Nirvana, and Pearl Jam albums and performances infiltrated radio, television, and concert halls, there was no shortage of media coverage. From 1992 through 1994, grunge bands were mentioned or featured on the cover of Rolling Stone 33 times (Hillburn). That same year, The New York Times ran the article “Grunge: A Success Story” featuring a short history of the Seattle sound, along with a “lexicon of grunge speak” (Marin), a joke perpetrated by a former 25-year-old Sub Pop employee, Megan Jasper, who never imagined her list of made-up vocabulary given to a New York Times reporter would grace the front page of the style section (Yarm). In their rush to keep up with pervasiveness of grunge culture, even The New York Times fell prey to Gen Xer’s comical cynicism.The circle of friends I ran with were split down the middle between Nirvana and Pearl Jam, a preference for one over the other, as the two bands and their respective front men garnered much of the media attention. Nirvana seemed to appeal to people’s sense of authenticity, perhaps more relatable in their aloofness to mainstream popularity, backed up with Cobain’s simple-yet-brilliant song arrangements and revealing lyrics. Lawrence Grossberg suggests that music fans recognise the difference between authentic and hom*ogenised rock, interpreting and aligning these differences with rock and roll’s association with “resistance, refusal, alienation, marginality, and so on” (62). I tended to gravitate toward Nirvana’s sound, mostly for technical reasons. Nevermind sparkled with aggressive guitar tones while capturing the power and fragility of Cobain’s voice. For many critics, the brilliance of Pearl Jam’s first album suffered from too much echo and reverb muddling the overall production value, but twenty years later they would remix and re-release Ten, correcting these production issues.Grunge FashionAs the music carved out a huge section of the charts, the grunge look was appropriated on fashion runways. When Cobain appeared on MTV wearing a ragged olive green cardigan he’d created a style simply by rummaging through his closet. Vedder and Cornell sported army boots, cargo shorts, and flannel shirts, suitable attire for the overcast climate of the Pacific Northwest, but their everyday garb turned into a fashion trend for Gen Xers that was then milked by designers. In 1992, the editor of Details magazine, James Truman, called grunge “un fashion” (Marin) as stepping out in second-hand clothes ran “counter to the shellacked, flashy aesthetic of 1980s” (Nnadi) for those who preferred “the waif-like look of put-on poverty” (Brady). But it was MTV’s relentless airing of Nirvana, Pearl Jam, and Soundgarden videos that sent Gen Xers flocking to malls and thrift-stores in search grunge-like apparel. I purchased a pair of giant, heavyweight Red Wing boots that looked like small cars on my feet, making it difficult to walk, but at least I was prepared for any terrain in all types of weather. The flannel came next; I still wear flannos. Despite its association with dark, murky musical themes, grunge kept me warm and dry.Much of grunge’s appeal to the masses was that it was not gender-specific; men and women dressed to appear unimpressed, sharing a taste for shapeless garments and muted colors without reference to stereotypical masculine or feminine styles. Cobain “allowed his own sexuality to be called into question by often wearing dresses and/or makeup on stage, in film clips, and on photo shoots, and wrote explicitly feminist songs, such as ‘Sappy’ or ‘Been a Son’” (Strong 403). I remember watching Pearl Jam’s 1992 performance on MTV Unplugged, seeing Eddie Vedder scrawl the words “Pro Choice” in black marker on his arm in support of women’s rights while his lyrics in songs like “Daughter”, “Better Man”, and “Why Go” reflected an equitable, humanistic if somewhat tragic perspective. Females and males moshed alongside one another, sharing the same spaces while experiencing and voicing their own response to grunge’s aggressive sound. Unlike the hypersexualised hair-metal bands of the 1980s whose aesthetic motifs often portrayed women as conquests or as powerless décor, the message of grunge rock avoided gender exploitation. As the ‘90s unfolded, underground feminist punk bands of the riot grrrl movement like Bikini Kill, L7, and Babes in Toyland expressed female empowerment with raging vocals and buzz-saw guitars that paved the way for Hole, Sleater-Kinney and other successful female-fronted grunge-era bands. The Decline of GrungeIn 1994, Kurt Cobain appeared on the cover of Newsweek magazine in memoriam after committing suicide in the greenhouse of his Seattle home. Mass media quickly spread the news of his passing internationally. Two days after his death, 7,000 fans gathered at Seattle Center to listen to a taped recording of Courtney Love, Cobain’s wife, a rock star in her own right, reading the suicide note he left behind.A few days after Cobain’s suicide, I found myself rolling down the highway with a carload of friends, one of my favorite Nirvana tunes, “Come As You Are” fighting through static. I fiddled with the radio to clear up the signal. The conversation turned to Cobain as we cobbled together the details of his death. I remember the chatter quieting down, Cobain’s voice fading as we gazed out the window at the empty terrain passing. In that reflective moment, I felt like I had experienced an intense, emotional relationship that came to an abrupt end. This “illusion of intimacy” (Horton and Wohl 217) between myself and Cobain elevated the loss I felt with his passing even though I had no intimate, personal ties to him. I counted this person as a friend (Giles 284) because I so closely identified with his words and music. I could not help but feel sad, even angry that he’d decided to end his life.Fueled by depression and a heroin addiction, Cobain’s death signaled an end to grunge’s collective appeal while shining a spotlight on one of the more dangerous aspects of its ethos. A 1992 Rolling Stone article mentioned that several of Seattle’s now-famous international musicians used heroin and “The feeling around town is, the drug is a disaster waiting to happen” (Azzerad). In 2002, eight years to the day of Cobain’s death, Layne Staley, lead singer of Alice In Chains, another seminal grunge outfit, was found dead of a suspected heroin overdose (Wiederhorn). When Cornell took his own life in 2017 after a long battle with depression, The Washington Post said, “The story of grunge is also one of death” (Andrews). The article included a Tweet from a grieving fan that read “The voices I grew up with: Andy Wood, Layne Staley, Chris Cornell, Kurt Cobain…only Eddie Vedder is left. Let that sink in” (@ThatEricAlper).ConclusionThe grunge movement of the early 1990s emerged out of musical friendships content to be on their own, on the outside, reflecting a sense of isolation and alienation in the music they made. As Cornell said, “We’ve always been fairly reclusive and damaged” (Foege). I felt much the same way in those days, sequestered in the desert, planting my grunge flag in the middle of country music territory, doing what I could to resist the status quo. Cobain, Cornell, Staley, and Vedder wrote about their own anxieties in a way that felt intimate and relatable, forging a bond with their fan base. Christopher Perricone suggests, “the relationship of an artist and audience is a collaborative one, a love relationship in the sense, a friendship” (200). In this way, grunge would become a shared memory among friends who rode the wave of this cultural phenomenon all the way through to its tragic consequences. But the music has survived. Along with my flannel shirts and Red Wing boots.References@ThatEricAlper (Eric Alper). “The voices I grew up with: Andy Wood, Layne Staley, Chris Cornell, Kurt Cobain…only Eddie Vedder is left. Let that sink in.” Twitter, 18 May 2017, 02:41. 15 Sep. 2018 <https://twitter.com/ThatEricAlper/status/865140400704675840?ref_src>.Andrews, Travis M. “After Chris Cornell’s Death: ‘Only Eddie Vedder Is Left. Let That Sink In.’” The Washington Post, 19 May 2017. 29 Aug. 2018 <https://www.washingtonpost.com/newsmorning-mix/wp/2017/05/19/after-chris-cornells-death-only-eddie-vedder-is-left-let-that-sink-in>.Azzerad, Michael. “Grunge City: The Seattle Scene.” Rolling Stone, 16 Apr. 1992. 20 Aug. 2018 <https://www.rollingstone.com/music/music-news/grunge-city-the-seattle-scene-250071/>.Brady, Diane. “Kids, Clothes and Conformity: Teens Fashion and Their Back-to-School Looks.” Maclean’s, 6 Sep. 1993. Brodeur, Nicole. “Chris Cornell: Soundgarden’s Dark Knight of the Grunge-Music Scene.” Seattle Times, 18 May 2017. 20 Aug. 2018 <https://www.seattletimes.com/entertainment/music/chris-cornell-soundgardens-dark-knight-of-the-grunge-music-scene/>.Ellis, Carolyn, and Arthur P. Bochner. “Autoethnography, Personal Narrative, Reflexivity: Researcher as Subject.” Handbook of Qualitative Research. 2nd ed. Eds. Norman Denzin and Yvonna Lincoln. Thousand Oaks, CA: Sage, 2000. 733-768.Foege, Alec. “Chris Cornell: The Rolling Stone Interview.” Rolling Stone, 28 Dec. 1994. 12 Sep. 2018 <https://www.rollingstone.com/music/music-features/chris-cornell-the-rolling-stone-interview-79108/>.Fricke, David. “Ten.” Rolling Stone, 12 Dec. 1991. 18 Sep. 2018 <https://www.rollingstone.com/music/music-album-reviews/ten-251421/>.Giles, David. “Parasocial Interactions: A Review of the Literature and a Model for Future Research.” Media Psychology 4 (2002): 279-305.Giles, Jeff. “The Poet of Alientation.” Newsweek, 17 Apr. 1994, 4 Sep. 2018 <https://www.newsweek.com/poet-alienation-187124>.Gross, D.M., and S. Scott. Proceding with Caution. Time, 16 July 1990. 3 Sep. 2018 <http://content.time.com/time/magazine/article/0,9171,155010,00.html>.Grossberg, Lawrence. “Is There a Fan in the House? The Affective Sensibility of Fandom. The Adoring Audience” Fan Culture and Popular Media. Ed. Lisa A. Lewis. New York, NY: Routledge, 1992. 50-65.Hillburn, Robert. “The Rise and Fall of Grunge.” Los Angeles Times, 21 May 1998. 20 Aug. 2018 <http://articles.latimes.com/1998/may/31/entertainment/ca-54992>.Horton, Donald, and R. Richard Wohl. “Mass Communication and Para-Social Interactions: Observations on Intimacy at a Distance.” Psychiatry: Interpersonal and Biological Process 19 (1956): 215-229.Latysheva, T.V. “The Essential Nature and Types of the Youth Subculture Phenomenon.” Russian Education and Society 53 (2011): 73–88.Manning, Jimmie, and Tony Adams. “Popular Culture Studies and Autoethnography: An Essay on Method.” The Popular Culture Studies Journal 3.1-2 (2015): 187-222.Marin, Rick. “Grunge: A Success Story.” New York Times, 15 Nov. 1992. 12 Sep. 2018 <https://www.nytimes.com/1992/11/15/style/grunge-a-success-story.html>.Neumann, Mark. “Collecting Ourselves at the End of the Century.” Composing Ethnography: Alternative Forms of Qualitative Writing. Eds. Carolyn Ellis and Arthur P. Bochner. London: Alta Mira Press, 1996. 172-198.Nirvana. "Smells Like Teen Spirit." Nevermind, Geffen, 1991.Nnadi, Chioma. “Why Kurt Cobain Was One of the Most Influential Style Icons of Our Times.” Vogue, 8 Apr. 2014. 15 Aug. 2018 <https://www.vogue.com/article/kurt-cobain-legacy-of-grunge-in-fashion>.Perricone, Christopher. “Artist and Audience.” The Journal of Value Inquiry 24 (2012). 12 Sep. 2018 <https://link.springer.com/content/pdf/10.1007/BF00149433.pdf>.Robbins, Ira. “Ten.” Rolling Stone, 12 Dec. 1991. 15 Aug. 2018 <https://www.rollingstone.com/music/music-album-reviews/ten-25142>.Strong, Catherine. “Grunge, Riott Grrl and the Forgetting of Women in Popular Culture.” The Journal of Popular Culture 44.2 (2011): 398-416. Wiederhorn, Jon. “Remembering Layne Staley: The Other Great Seattle Musician to Die on April 5.” MTV, 4 June 2004. 23 Sep. 2018 <http://www.mtv.com/news/1486206/remembering-layne-staley-the-other-great-seattle-musician-to-die-on-april-5/>.Yarm, Mark. Everybody Loves Our Town: An Oral History of Grunge. Three Rivers Press, 2011.

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Abstract:

“The cop in our head represses us better than any police force. Through generations of conditioning, the system has created people who have a very hard time coming together to create resistance.” – Seth Tobocman, War in the Neighborhood (1999) Even when creators of autobiographically-based comics claim to depict real events, their works nonetheless inspire confrontations as a result of ideological contestations which position them, on the one hand, as popular culture, and, on the other hand, as potentially subversive material for adults. In Seth Tobocman’s War in the Neighborhood (1999), the street politics in which Tobocman took part extends the graphic novel narrative to address personal experiences as seen through a social lens both political and fragmented by the politics of relationships. Unlike Art Spiegelman’s Maus (1986, 1991), War in the Neighborhood is situated locally and with broader frames of reference, but, like Maus, resonates globally across cultures. Because Tobocman figures the street as the primary site of struggle, John Street’s historiographically-oriented paper, “Political Culture – From Civic Culture to Mass Culture”, presents a framework for understanding not that symbols determine action, any more than material or other objective conditions do, but rather that there is a constant process of interpretation and reinterpretation which is important to the way actors view their predicament and formulate their intentions. (107-108) Though Street’s main focus is on the politicization of choices involving institutional structures, his observation offers a useful context to examining Tobocman’s memoir of protest in New York City. Tobocman’s identity as an artist, however, leads him to caution his readers: Yes, it [War in the Neighborhood] is based on real situations and events, just as a landscape by Van Gogh may be based on a real landscape. But we would not hire Van Gogh as a surveyor on the basis of those paintings. (From the “Disclaimer” on the copyright page.) This speaks to the reality that all art, no matter how innocuously expressed, reflect interpretations refracted from the artists’ angles. It also calls attention to the individual artist’s intent. For Tobocman, “I ask that these stories be judged not on how accurately they depict particular events, but on what they contain of the human spirit” (from the “Disclaimer” on the copyright page). War in the Neighborhood, drawn in what appears to be pencil and marker, alternates primarily between solidly-inked black generic shapes placed against predominantly white backgrounds (chapters 1-3, 5, 7-9, and 11) and depth-focused drawing-quality images framed against mostly black backgrounds (chapters 4 and 6); chapter 10 represents an anomaly because it features typewritten text and photographs that reify the legitimacy of the events portrayed even when “intended to be a work of art” (from the “Disclaimer” on the copyright page). According to Luc Sante’s “Introduction”, “the high-contrast images here are descended from the graphic vocabulary of Masereel and Lynn Ward, an efficient and effective means of representing the war of body and soul” (n.p.). This is especially evident in the last page of War in the Neighborhood, where Tobocman bleeds himself through four panels, the left side of his body dressed in skin with black spaces for bone and the right side of his body skeletonized against his black frame (panels 5-6: 328). For Tobocman, “the war of body and soul” reifies the struggle against the state, through which its representatives define people as capital rather than as members of a social contract. Before the second chapter, however, Tobocman introduces New York squatter, philosopher and teacher Raphael Bueno’s tepee-embedded white-texted poem, “‘Nine-Tenths of the Law’” (29). Bueno’s words eloquently express the heart behind War in the Neighborhood, but could easily be dismissed because they take up only one page. The poem’s position is significant, however. It reflects the struggles between agency and class, between power and oppression, and between capitalism and egalitarianism. Tobocman includes a similar white-texted tepee in Chapter 4, though the words are not justified and the spacing between the words and the edges of the tepee are larger. In this chapter, Tobocman focuses on the increasing media attention given to the Thompson Square Park homeless, who first organize as “the Homeless Clients Advisory Board” (panel 7: 86). The white-texted tepee reads: They [Tent City members] got along well with the Chinese students, participated in free China rallys, learned to say ‘Down with Deng Xiao ping’ in Chinese. It was becoming clear to Tent City that their homelessness meant some thing on a world stage. (panel 6: 103) The OED Online cites 1973 as the first use of gentrification, which appeared in “Times 26 Sept. 19/3.” It also lists uses in 1977, 1982 and 1985. While the examples provided point to business-specific interests associated with gentrification, it is now defined as “the process by which an (urban) area is rendered middle-class.” While gentrification, thus, infers the displacement of minority members for the benefits of white privilege, it is also complicated by issues of eminent domain. For the disenfranchised who lack access to TV, radio and other venues of public expression (i.e., billboards), “taking it to the streets” means trafficking ideas, grievances and/or evangelisms. In places like NYC, the nexus for civic engagement is the street. The main thrust of Tobocman’s War in the Neighborhood, however, centers on the relationships between (1) the squatters, against whom Reagan-era economics destabilized, (2) the police, whose roles changed as local policies shifted to accommodate urban planning, (3) the politicians, who “began to campaign to destroy innercity neighborhoods” (20), and (4) the media, which served elitist interests. By chapter 3, Tobocman intrudes himself into the narrative to personalize the story of squatters and their resistance of an agenda that worked to exclude them. In chapter 4, he intersects the interests of squatters with the homeless. With chapter 5, Tobocman, already involved, becomes a squatter, too; however, he also maintains his apartment, making him both an insider and an outsider. The meta-discourses include feminism, sexism and racism, entwined concepts usually expressed in opposition. Fran is a feminist who demands not only equality for women, but also respect. Most of the men share traditional values of manhood. Racism, while recognized at a societal level, creeps into the choices concerning the dismissal or acceptance of blacks and whites at ABC House on 13th Street, where Tobocman resided. As if speaking to an interviewer, a black woman explains, as a white male, his humanity had a full range of expression. But to be a black person and still having that full range of expression, you were punished for it. ... It was very clear that there were two ways of handling people who were brought to the building. (full-page panel: 259) Above the right side of her head is a yin yang symbol, whose pattern contrasts with the woman’s face, which also shows shading on the right side. The yin yang represents equanimity between two seemingly opposing forces, yet they cannot exist without the other; it means harmony, but also relation. This suggests balance, as well as a shared resistance for which both sides of the yin yang maintain their identities while assuming community within the other. However, as Luc Sante explains in his “Introduction” to War in the Neighborhood, the word “community” gets thrown around with such abandon these days it’s difficult to remember that it has ever meant anything other than a cluster of lobbyists. ... A community is in actuality a bunch of people whose intimate lives rub against one another’s on a daily basis, who possess a common purpose not unmarred by conflict of all sizes, who are thus forced to negotiate their way across every substantial decision. (n.p., italics added) The homeless organized among themselves to secure spaces like Tent House. The anarchists lobbied the law to protect their squats. The residents of ABC House created rules to govern their behaviors toward each other. In all these cases, they eventually found dissent among themselves. Turning to a sequence on the mayoral transition from Koch to Dinkins, Tobocman likens “this inauguration day” as a wedding “to join this man: David Dinkins…”, “with the governmental, business and real estate interests of New York City” (panel 1: 215). Similarly, ABC House, borrowing from the previous, tried to join with the homeless, squatters and activist organizations, but, as many lobbyists vying for the same privilege, contestations within and outside ABC splintered the goal of unification. Yet the street remains the focal point of War in the Neighborhood. Here, protests and confrontations with the police, who acted as intermediary agents for the politicians, make the L.E.S. (Lower East Side) a site of struggle where ordinary activities lead to war. Though the word war might otherwise seem like an exaggeration, Tobocman’s inclusion of a rarely seen masked figure says otherwise. This “t-shirt”-hooded (panel 1: 132) wo/man, one of “the gargoyles, the defenders of the buildings” (panel 3: 132), first appears in panel 3 on page 81 as part of this sequence: 319 E. 8th Street is now a vacant lot. (panel 12: 80) 319 taught the squatters to lock their doors, (panel 1: 81) always keep a fire extinguisher handy, (panel 2: 81) to stay up nights watching for the arsonist. (panel 3: 81) Never to trust courts cops, politicians (panel 4: 81) Recognize a state of war! (panel 5: 81) He or she reappears again on pages 132 and 325. In Fernando Calzadilla’s “Performing the Political: Encapuchados in Venezuela”, the same masked figures can be seen in the photographs included with his article. “Encapuchados,” translates Calzadilla, “means ‘hooded ones,’ so named because of the way the demonstrators wrap their T-shirts around their faces so only their eyes show, making it impossible for authorities to identify them” (105). While the Encapuchados are not the only group to dress as such, Tobocman’s reference to that style of dress in War in the Neighborhood points to the dynamics of transculturation and the influence of student movements on the local scene. Student movements, too, have traditionally used the street to challenge authority and to disrupt its market economy. More important, as Di Wang argues in his book Street Culture in Chengdu: Public Space, Urban Commoners, and Local Politics, 1870-1930, in the process of social transformation, street culture was not only the basis for commoners’ shared identity but also a weapon through which they simultaneously resisted the invasion of elite culture and adapted to its new social, economic, and political structures. (247) While focusing on the “transformation that resulted in the reconstruction of urban public space, re-creation of people’s public roles, and re-definition of the relationship among ordinary people, local elites and the state” (2), Wang looks at street culture much more broadly than Tobocman. Though Wang also connects the 1911 Revolution as a response to ethnic divisions, he examines in greater detail the everyday conflicts concerning local identities, prostitutes in a period marked by increasing feminisms, beggars who organized for services and food, and the role of tea houses as loci of contested meanings. Political organization, too, assumes a key role in his text. Similarly to Wang, what Tobocman addresses in War in the Neighborhood is the voice of the subaltern, whose street culture is marked by both social and economic dimensions. Like the poor in New York City, the squatters in Iran, according to Asef Bayat in his article “Un-Civil Society: The Politics of the ‘Informal People’”, “between 1976 and the early 1990s” (53) “got together and demanded electricity and running water: when they were refused or encountered delays, they resorted to do-it-yourself mechanisms of acquiring them illegally” (54). The men and women in Tobocman’s War in the Neighborhood, in contrast, faced barricaded lines of policemen on the streets, who struggled to keep them from getting into their squats, and also resorted to drastic measures to keep their buildings from being destroyed after the court system failed them. Should one question the events in Tobocman’s comics, however, he or she would need to go no further than Hans Pruijt’s article, “Is the Institutionalization of Urban Movements Inevitable? A Comparison of the Opportunities for Sustained Squatting in New York City and Amsterdam”: In the history of organized squatting on the Lower East Side, squatters of nine buildings or clusters of buildings took action to avert threat of eviction. Some of the tactics in the repertoire were: Legal action; Street protest or lock-down action targeting a (non-profit) property developer; Disruption of meetings; Non-violent resistance (e.g. placing oneself in the way of a demolition ball, lining up in front of the building); Fortification of the building(s); Building barricades in the street; Throwing substances at policemen approaching the building; Re-squatting the building after eviction. (149) The last chapter in Tobocman’s War in the Neighborhood, chapter 11: “Conclusion,” not only plays on the yin and yang concept with “War in the Neighborhood” in large print spanning two panels, with “War in the” in white text against a black background and “Neighborhood” in black text against a white background (panels 3-4: 322), but it also shows concretely how our wars against each other break us apart rather than allow us to move forward to share in the social contract. The street, thus, assumes a meta-narrative of its own: as a symbol of the pathways that can lead us in many directions, but through which we as “the people united” (full-page panel: 28) can forge a common path so that all of us benefit, not just the elites. Beyond that, Tobocman’s graphic novel travels through a world of activism and around the encounters of dramas between people with different goals and relationships to themselves. Part autobiography, part documentary and part commentary, his graphic novel collection of his comics takes the streets and turns them into a site for struggle and dislocation to ask at the end, “How else could we come to know each other?” (panel 6: 328). Tobocman also shapes responses to the text that mirror the travesty of protest, which brings discord to a world that still privileges order over chaos. Through this reconceptualization of a past that still lingers in the present, War in the Neighborhood demands a response from those who would choose “to take up the struggle against oppression” (panel 3: 328). In our turn, we need to recognize that the divisions between us are shards of the same glass. References Bayat, Asef. “Un-Civil Society: The Politics of the “Informal People.’” Third World Quarterly 18.1 (1997): 53-72. Calzadilla, Fernando. “Performing the Political: Encapuchados in Venezuela.” The Drama Review 46.4 (Winter 2002): 104-125. “Gentrification.” OED Online. 2nd Ed. (1989). http://0-dictionary.oed.com.csulib.ctstateu.edu/ cgi/entry/50093797?single=1&query_type=word&queryword=gentrification &first=1&max_to_show=10>. 25 Apr. 2006. Pruijt, Hans. “Is the Institutionalization of Urban Movements Inevitable? A Comparison of the Opportunities for Sustained Squatting in New York and Amsterdam.” International Journal of Urban and Regional Research 27.1 (Mar. 2003): 133-157. Street, John. “Political Culture – From Civic to Mass Culture.” British Journal of Political Science 24.1 (Jan. 1994): 95-113. Toboman, Seth. War in the Neighborhood (chapter 1 originally published in Squatter Comics, no. 2 (Photo Reference provided by City Limits, Lower East Side Anti-displacement Center, Alan Kronstadt, and Lori Rizzo; Book References: Low Life, by Luc Sante, Palante (the story of the Young Lords Party), Squatters Handbook, Squatting: The Real Story, and Sweat Equity Urban Homesteading; Poem, “‘Nine-Tenths of the Law,’” by Raphael Bueno); chapter 2 (Inkers: Samantha Berger, Lasante Holland, Becky Minnich, Ursula Ostien, Barbara Lee, and Seth Tobocman; Photo Reference: the daily papers, John Penley, Barbara Lee, Paul Kniesel, Andrew Grossman, Peter LeVasseur, Betsy Herzog, William Comfort, and Johannes Kroemer; Page 81: Assistant Inker: Peter Kuper, Assistant Letterer: Sabrina Jones and Lisa Barnstone, Photo Reference: Paul Garin, John Penley, and Myron of E.13th St); chapter 3 originally published in Heavy Metal 15, no. 11 (Inkers: Peter Kuper and Seth Tobocman; Letterers: Sabrina Jones, Lisa Barnstone, and Seth Tobocman; Photo Reference: Paul Garin, John Penley, Myron of 13th Street, and Mitch Corber); chapter 4 originally published in World War 3 Illustrated, no. 21 (Photo Reference: John Penley, Andrew Lichtenstein, The Shadow, Impact Visuals, Paper Tiger TV, and Takeover; Journalistic Reference: Sarah Ferguson); chapter 5 originally published in World War 3 Illustrated, no. 13, and reprinted in World War 3 Illustrated Confrontational Comics, published by Four Walls Eight Windows (Photo Reference: John Penley and Chris Flash (The Shadow); chapter 6 (Photo reference: Clayton Patterson (primary), John Penley, Paul Garin, Andrew Lichtenstein, David Sorcher, Shadow Press, Impact Visuals, Marianne Goldschneider, Mike Scott, Mitch Corber, Anton Vandalen, Paul Kniesel, Chris Flash (Shadow Press), and Fran Luck); chapter 7 (Photo Reference: Sarah Teitler, Marianne Goldschneider, Clayton Patterson, Andrew Lichtenstein, David Sorcher, John Penley, Paul Kniesel, Barbara Lee, Susan Goodrich, Sarah Hogarth, Steve Ashmore, Survival Without Rent, and Bjorg; Inkers: Ursula Ostien, Barbara Lee, Samantha Berger, Becky Minnich, and Seth Tobocman); chapter 8 originally published in World War 3 Illustrated, no. 15 (Inkers: Laird Ogden and Seth Tobocman; Photo Reference: Paul Garin, Clayton Patterson, Paper Tiger TV, Shadow Press, Barbara Lee, John Penley, and Jack Dawkins; Collaboration on Last Page: Seth Tobocman, Zenzele Browne, and Barbara Lee); chapter 9 originally published in Real Girl (Photo Reference: Sarah Teitler and Barbara Lee); chapter 10 (Photos: John Penley, Chris Egan, and Scott Seabolt); chapter 11: “Conclusion” (Inkers: Barbara Lee, Laird Ogden, Samantha Berger, and Seth Tobocman; Photo Reference: Anton Vandalen). Intro. by Luc Sante. Computer Work: Eric Goldhagen and Ben Meyers. Text Page Design: Jim Fleming. Continuous Tone Prints and Stats Shot at Kenfield Studio: Richard Darling. Brooklyn, NY: Autonomedia, 1999. Wang, Di. Street Culture in Chengdu: Public Space, Urban Commoners, and Local Politics, 1870-1930. Stanford, CA: Stanford UP, 2003. Citation reference for this article MLA Style Raney, Vanessa. "Where Ordinary Activities Lead to War: Street Politics in Seth Tobocman’s War in the Neighborhood." M/C Journal 9.3 (2006). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0607/01-raney.php>. APA Style Raney, V. (Jul. 2006) "Where Ordinary Activities Lead to War: Street Politics in Seth Tobocman’s War in the Neighborhood," M/C Journal, 9(3). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0607/01-raney.php>.

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Lincolnshire, England. The crowd cheer when the ball breaks loose. From one end of the field to the other, the players chase, their snouts hovering just above the grass. It’s not a case of four legs being better, rather a novel way to attract customers to the Woodside Wildlife and Falconry Park. During the matches, volunteers are drawn from the crowd to hold goal posts at either end of the run the pigs usually race on. With five pigs playing, two teams of two and a referee, and a ball designed to leak feed as it rolls (Stevenson) the ten-minute competition is fraught with tension. While the pig’s contributions to “the beautiful game” (Fish and Pele 7) have not always been so obvious, it could be argued that specific parts of the animal have had a significant impact on a sport which, despite calls to fall into line with much of the rest of the world, people in Australia (and the US) are more likely to call soccer. The Football Precursors to the modern football were constructed around an inflated pig’s bladder (Price, Jones and Harland). Animal hide, usually from a cow, was stitched around the bladder to offer some degree of stability, but the bladder’s irregular and uneven form made for unpredictable movement in flight. This added some excitement and affected how ball games such as the often violent, calico matches in Florence, were played. In the early 1970s, the world’s oldest ball was discovered during a renovation in Stirling Castle, Scotland. The ball has a pig’s bladder inside its hand-stitched, deer-hide outer. It was found in the ceiling above the bed in, what was then Mary Queens of Scots’ bedroom. It has since been dated to the 1540s (McGinnes). Neglected and left in storage until the late 1990s, the ball found pride of place in an exhibition in the Smiths Art Gallery and Museum, Stirling, and only gained worldwide recognition (as we will see later) in 2006. Despite confirmed interest in a number of sports, there is no evidence to support Mary’s involvement with football (Springer). The deer-hide ball may have been placed to gather and trap untoward spirits attempting to enter the monarch’s sleep, or simply left by accident and forgotten (McGinnes in Springer). Mary, though, was not so fortunate. She was confined and forgotten, but only until she was put to death in 1587. The Executioner having gripped her hair to hold his prize aloft, realised too late it was a wig and Mary’s head bounced and rolled across the floor. Football Development The pig’s bladder was the central component in the construction of the football for the next three hundred years. However, the issue of the ball’s movement (the bounce and roll), the bladder’s propensity to burst when kicked, and an unfortunate wife’s end, conspired to push the pig from the ball before the close of the nineteenth-century. The game of football began to take its shape in 1848, when JC Thring and a few colleagues devised the Cambridge Rules. This compromised set of guidelines was developed from those used across the different ‘ball’ games played at England’s elite schools. The game involved far more kicking, and the pig’s bladders, prone to bursting under such conditions, soon became impractical. Charles Goodyear’s invention of vulcanisation in 1836 and the death of prestigious rugby and football maker Richard Lindon’s wife in 1870 facilitated the replacement of the animal bladder with a rubber-based alternative. Tragically, Mr Lindon’s chief inflator died as a result of blowing up too many infected pig’s bladders (Hawkesley). Before it closed earlier this year (Rhoads), the US Soccer Hall of Fame displayed a rubber football made in 1863 under the misleading claim that it was the oldest known football. By the late 1800s, professional, predominantly Scottish play-makers had transformed the game from its ‘kick-and-run’ origins into what is now called ‘the passing game’ (Sanders). Football, thanks in no small part to Scottish factory workers (Kay), quickly spread through Europe and consequently the rest of the world. National competitions emerged through the growing need for organisation, and the pig-free mass production of balls began in earnest. Mitre and Thomlinson’s of Glasgow were two of the first to make and sell their much rounder balls. With heavy leather panels sewn together and wrapped around a thick rubber inner, these balls were more likely to retain shape—a claim the pig’s bladder equivalent could not legitimately make. The rubber-bladdered balls bounced more too. Their weight and external stitching made them more painful to header, but also more than useful for kicking and particularly for passing from one player to another. The ball’s relatively quick advancement can thereafter be linked to the growth and success of the World Cup Finals tournament. Before the pig re-enters the fray, it is important to glance, however briefly, at the ball’s development through the international game. World Cup Footballs Pre-tournament favourites, Spain, won the 2010 FIFA World Cup, playing with “an undistorted, perfectly spherical ball” (Ghosh par. 7), the “roundest” ever designed (FIFA par.1). Their victory may speak to notions of predictability in the ball, the tournament and the most lucrative levels of professional endeavour, but this notion is not a new one to football. The ball’s construction has had an influence on the way the game has been played since the days of Mary Queen of Scots. The first World Cup Final, in 1930, featured two heavy, leather, twelve-panelled footballs—not dissimilar to those being produced in Glasgow decades earlier. The players and officials of Uruguay and Argentina could not agree, so they played the first half with an Argentine ball. At half-time, Argentina led by two goals to one. In the second half, Uruguay scored three unanswered goals with their own ball (FIFA). The next Final was won by Italy, the home nation in 1934. Orsi, Italy’s adopted star, poked a wildly swerving shot beyond the outstretched Czech keeper. The next day Orsi, obligated to prove his goal was not luck or miracle, attempted to repeat the feat before an audience of gathered photographers. He failed. More than twenty times. The spin on his shot may have been due to the, not uncommon occurrence, of the ball being knocked out of shape during the match (FIFA). By 1954, the Federation Internationale de Football Association (FIFA) had sought to regulate ball size and structure and, in 1958, rigorously tested balls equal to the demands of world-class competition. The 1950s also marked the innovation of the swerving free kick. The technique, developed in the warm, dry conditions of the South American game, would not become popular elsewhere until ball technology improved. The heavy hand-stitched orb, like its early counterparts, was prone to water absorption, which increased the weight and made it less responsive, particularly for those playing during European winters (Bray). The 1970 World Cup in Mexico saw football progress even further. Pele, arguably the game’s greatest player, found his feet, and his national side, Brazil, cemented their international football prominence when they won the Jules Rimet trophy for the third time. Their innovative and stylish use of the football in curling passes and bending free kicks quickly spread to other teams. The same World Cup saw Adidas, the German sports goods manufacturer, enter into a long-standing partnership with FIFA. Following the competition, they sold an estimated six hundred thousand match and replica tournament footballs (FIFA). The ball, the ‘Telstar’, with its black and white hexagonal panels, became an icon of the modern era as the game itself gained something close to global popularity for the first time in its history. Over the next forty years, the ball became incrementally technologically superior. It became synthetic, water-resistant, and consistent in terms of rebound and flight characteristics. It was constructed to be stronger and more resistant to shape distortion. Internal layers of polyutherane and Syntactic Foam made it lighter, capable of greater velocity and more responsive to touch (FIFA). Adidas spent three years researching and developing the 2006 World Cup ball, the ‘Teamgeist’. Fourteen panels made it rounder and more precise, offering a lower bounce, and making it more difficult to curl due to its accuracy in flight. At the same time, audiences began to see less of players like Roberto Carlos (Brazil and Real Madrid CF) and David Beckham (Manchester United, LA Galaxy and England), who regularly scored goals that challenged the laws of physics (Gill). While Adidas announced the 2006 release of the world’s best performing ball in Berlin, the world’s oldest was on its way to the Museum fur Volkerkunde in Hamburg for the duration of the 2006 FIFA World Cup. The Mary Queen of Scot’s ball took centre spot in an exhibit which also featured a pie stand—though not pork pies—from Hibernian Football Club (Strang). In terms of publicity and raising awareness of the Scots’ role in the game’s historical development, the installation was an unrivalled success for the Scottish Football Museum (McBrearty). It did, however, very little for the pig. Heads, not Tails In 2002, the pig or rather the head of a pig, bounced and rolled back into football’s limelight. For five years Luis Figo, Portugal’s most capped international player, led FC Barcelona to domestic and European success. In 2000, he had been lured to bitter rivals Real Madrid CF for a then-world record fee of around £37 million (Nash). On his return to the Catalan Camp Nou, wearing the shimmering white of Real Madrid CF, he was showered with beer cans, lighters, bottles and golf balls. Among the objects thrown, a suckling pig’s head chimed a psychological nod to the spear with two sharp ends in William Golding’s story. Play was suspended for sixteen minutes while police tried to quell the commotion (Lowe). In 2009, another pig’s head made its way into football for different reasons. Tightly held in the greasy fingers of an Orlando Pirates fan, it was described as a symbol of the ‘roasting’ his team would give the Kaiser Chiefs. After the game, he and his friend planned to eat their mascot and celebrate victory over their team’s most reviled competitors (Edwards). The game ended in a nil-all draw. Prior to the 2010 FIFA World Cup, it was not uncommon for a range of objects that European fans might find bizarre, to be allowed into South African league matches. They signified luck and good feeling, and in some cases even witchcraft. Cabbages, known locally for their medicinal qualities, were very common—common enough for both sets of fans to take them (Edwards). FIFA, an organisation which has more members than the United Nations (McGregor), impressed their values on the South African Government. The VuVuZela was fine to take to games; indeed, it became a cultural artefact. Very little else would be accepted. Armed with their economy-altering engine, the world’s most watched tournament has a tendency to get what it wants. And the crowd respond accordingly. Incidentally, the ‘Jabulani’—the ball developed for the 2010 tournament—is the most consistent football ever designed. In an exhaustive series of tests, engineers at Loughborough University, England, learned, among other things, the added golf ball-like grooves on its surface made the ball’s flight more symmetrical and more controlled. The Jabulani is more reliable or, if you will, more predictable than any predecessor (Ghosh). Spanish Ham Through support from their Governing body, the Real Federación Española de Fútbol, Spain have built a national side with experience, and an unparalleled number of talented individuals, around the core of the current FC Barcelona club side. Their strength as a team is founded on the bond between those playing on a weekly basis at the Catalan club. Their style has allowed them to create and maintain momentum on the international stage. Victorious in the 2008 UEFA European Football Championship and undefeated in their run through the qualifying stages into the World Cup Finals in South Africa, they were tournament favourites before a Jabulani was rolled into touch. As Tim Parks noted in his New York Review of Books article, “The Shame of the World Cup”, “the Spanish were superior to an extent one rarely sees in the final stages of a major competition” (2010 par. 15). They have a “remarkable ability to control, hold and hide the ball under intense pressure,” and play “a passing game of great subtlety [ ... to] patiently wear down an opposing team” (Parks par. 16). Spain won the tournament having scored fewer goals per game than any previous winner. Perhaps, as Parks suggests, they scored as often as they needed to. They found the net eight times in their seven matches (Fletcher). This was the first time that Spain had won the prestigious trophy, and the first time a European country has won the tournament on a different continent. In this, they have broken the stranglehold of superpowers like Germany, Italy and Brazil. The Spanish brand of passing football is the new benchmark. Beautiful to watch, it has grace, flow and high entertainment value, but seems to lack something of an organic nature: that is, it lacks the chance for things to go wrong. An element of robotic aptitude has crept in. This occurred on a lesser scale across the 2010 FIFA World Cup finals, but it is possible to argue that teams and players, regardless of nation, have become interchangeable, that the world’s best players and the way they play have become identikits, formulas to be followed and manipulated by master tacticians. There was a great deal of concern in early rounds about boring matches. The world’s media focused on an octopus that successfully chose the winner of each of Germany’s matches and the winner of the final. Perhaps, in shaping the ‘most’ perfect ball and the ‘most’ perfect football, the World Cup has become the most predictable of tournaments. In Conclusion The origins of the ball, Orsi’s unrepeatable winner and the swerving free kick, popular for the best part of fifty years, are worth remembering. These issues ask the powers of football to turn back before the game is smothered by the hunt for faultlessness. The unpredictability of the ball goes hand in hand with the game. Its flaws underline its beauty. Football has so much more transformative power than lucrative evolutionary accretion. While the pig’s head was an ugly statement in European football, it is a symbol of hope in its South African counterpart. Either way its removal is a reminder of Golding’s message and the threat of hom*ogeneity; a nod to the absence of the irregular in the modern era. Removing the curve from the free kick echoes the removal of the pig’s bladder from the ball. The fun is in the imperfection. Where will the game go when it becomes indefectible? Where does it go from here? Can there really be any validity in claiming yet another ‘roundest ball ever’? Chip technology will be introduced. The ball’s future replacements will be tracked by satellite and digitally-fed, reassured referees will determine the outcome of difficult decisions. Victory for the passing game underlines the notion that despite technological advancement, the game has changed very little since those pioneering Scotsmen took to the field. Shouldn’t we leave things the way they were? Like the pigs at Woodside Wildlife and Falconry Park, the level of improvement seems determined by the level of incentive. The pigs, at least, are playing to feed themselves. Acknowledgments The author thanks editors, Donna Lee Brien and Adele Wessell, and the two blind peer reviewers, for their constructive feedback and reflective insights. The remaining mistakes are his own. References “Adidas unveils Golden Ball for 2006 FIFA World Cup Final” Adidas. 18 Apr. 2006. 23 Aug. 2010 . Bray, Ken. “The science behind the swerve.” BBC News 5 Jun. 2006. 19 Aug. 2010 http://news.bbc.co.uk/2/hi/uk_news/magazine/5048238.stm>. Edwards, Piers. “Cabbage and Roasted Pig.” BBC Fast Track Soweto, BBC News 3 Nov. 2009. 23 Aug. 2010 . FIFA. “The Footballs during the FIFA World Cup™” FIFA.com. 18 Aug. 2010 .20 Fish, Robert L., and Pele. My Life and the Beautiful Game. New York: Bantam Dell, 1977. Fletcher, Paul. “Match report on 2010 FIFA World Cup Final between Spain and Netherlands”. BBC News—Sports 12 Jul. 2010 . Ghosh, Pallab. “Engineers defend World Cup football amid criticism.” BBC News—Science and Environment 4 Jun. 2010. 19 Aug. 2010 . Gill, Victoria. “Roberto Carlos wonder goal ‘no fluke’, say physicists.” BBC News—Science and Environment 2 Sep. 2010 . Hawkesley, Simon. Richard Lindon 22 Aug. 2010 . “History of Football” FIFA.com. Classic Football. 20 Aug. 2010 . Kay, Billy. The Scottish World: A Journey into the Scottish Diaspora. London: Mainstream, 2008. Lowe, Sid. “Peace for Figo? And pigs might fly ...” The Guardian (London). 25 Nov. 2002. 20 Aug. 2010 . “Mary, Queen of Scots (r.1542-1567)”. The Official Website of the British Monarchy. 20 Jul. 2010 . McBrearty, Richard. Personal Interview. 12 Jul. 2010. McGinnes, Michael. Smiths Art Gallery and Museum. Visited 14 Jul. 2010 . McGregor, Karen. “FIFA—Building a transnational football community. University World News 13 Jun. 2010. 19 Jul. 2010 . Nash, Elizabeth. “Figo defects to Real Madrid for record £36.2m." The Independent (London) 25 Jul. 2000. 20 Aug. 2010 . “Oldest football to take cup trip” 25 Apr. 2006. 20 Jul. 2010 . Parks, Tim. “The Shame of the World Cup”. New York Review of Books 19 Aug. 2010. 23 Aug. 2010 < http://nybooks.com/articles/archives/2010/aug/19/shame-world-cup/>. “Pig football scores a hit at centre.” BBC News 4 Aug. 2009. August 20 2010 . Price, D. S., Jones, R. Harland, A. R. “Computational modelling of manually stitched footballs.” Proceedings of the Institution of Mechanical Engineers, Part L. Journal of Materials: Design & Applications 220 (2006): 259-268. Rhoads, Christopher. “Forget That Trip You Had Planned to the National Soccer Hall of Fame.” Wall Street Journal 26 Jun. 2010. 22 Sep. 2010 . “Roberto Carlos Impossible Goal”. News coverage posted on You Tube, 27 May 2007. 23 Aug. 2010 . Sanders, Richard. Beastly Fury. London: Bantam, 2009. “Soccer to become football in Australia”. Sydney Morning Herald 17 Dec. 2004. 21 Aug. 2010 . Springer, Will. “World’s oldest football – fit for a Queen.” The Scotsman. 13 Mar. 2006. 19 Aug. 2010 < http://heritage.scotsman.com/willspringer/Worlds-oldest-football-fit.2758469.jp >. Stevenson, R. “Pigs Play Football at Wildlife Centre”. Lincolnshire Echo 3 Aug. 2009. 20 Aug. 2010 . Strang, Kenny. Personal Interview. 12 Jul. 2010. “The Execution of Mary Queen of Scots February 8, 1857”. Tudor History 21 Jul. 2010 http://tudorhistory.org/primary/exmary.html>. “The History of the FA.” The FA. 20 Jul. 2010 “World’s Oldest Ball”. World Cup South Africa 2010 Blog. 22 Jul. 2010 . “World’s Oldest Soccer Ball by Charles Goodyear”. 18 Mar. 2010. 20 Jul. 2010 .

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Introduction Darkness is often characterised as something that warrants heightened caution and scrutiny – signifying increased danger and risk. Within settler-colonial settings such as Australia, cautionary and negative connotations of darkness are projected upon Black people and their bodies, forming part of continuing colonial regimes of power (Moreton-Robinson). Negative stereotypes of “dark” continues to racialise all Indigenous peoples. In Australia, Indigenous peoples are both Indigenous and Black regardless of skin colour, and this plays out in a range of ways, some of which will be highlighted within this article. This article demonstrates that for Indigenous peoples, associations of fear and danger are built into the structural mechanisms that shape and maintain colonial understandings of Indigenous peoples and their bodies. It is this embodied form of darkness, and its negative connotations, and responses that we explore further. Figure 1: Megan Cope’s ‘I’m not afraid of the Dark’ t-shirt (Fredericks and Heemsbergen 2021) Responding to the anxieties and fears of settlers that often surround Indigenous peoples, Quandamooka artist and member of the art collective ProppaNow, Megan Cope, has produced a range of t-shirts, one of which declares “I’m not afraid of the Dark” (fig. 1). The wording ‘reflects White Australia’s fear of blackness’ (Dark + Dangerous). Exploring race relations through the theme of “darkness”, we begin by discussing how negative connotations of darkness are represented through everyday lexicons and how efforts to shift prejudicial and racist language are often met with defensiveness and resistance. We then consider how fears towards the dark translate into everyday practices, reinforced by media representations. The article considers how stereotype, conjecture, and prejudice is inflicted upon Indigenous people and reflects white settler fears and anxieties, rooting colonialism in everyday language, action, and norms. The Language of Fear Indigenous people and others with dark skin tones are often presented as having a proclivity towards threatening, aggressive, deceitful, and negative behaviours. This works to inform how Indigenous peoples are “known” and responded to by hegemonic (predominantly white) populations. Negative connotations of Indigenous people are a means of reinforcing and legitimising the falsity that European knowledge systems, norms, and social structures are superior whilst denying the contextual colonial circ*mstances that have led to white dominance. In Australia, such denial corresponds to the refusal to engage with the unceded sovereignty of Aboriginal peoples or acknowledge Indigenous resistance. Language is integral to the ways in which dominant populations come to “know” and present the so-called “Other”. Such language is reflected in digital media, which both produce and maintain white anxieties towards race and ethnicity. When part of mainstream vernacular, racialised language – and the value judgments associated with it – often remains in what Moreton-Robinson describes as “invisible regimes of power” (75). Everyday social structures, actions, and habits of thought veil oppressive and discriminatory attitudes that exist under the guise of “normality”. Colonisation and the dominance of Eurocentric ways of knowing, being, and doing has fixated itself on creating a normality that associates Indigeneity and darkness with negative and threatening connotations. In doing so, it reinforces power balances that presents an image of white superiority built on the invalidation of Indigeneity and Blackness. White fears and anxieties towards race made explicit through social and digital media are also manifest via subtle but equally pervasive everyday action (Carlson and Frazer; Matamoros-Fernández). Confronting and negotiating such fears becomes a daily reality for many Indigenous people. During the height of the 2020 Black Lives Matter protests in the United States, which extended to Australia and were linked to deaths in custody and police violence, African American poet Saul Williams reminded his followers of the power of language in constructing racialised fears (saulwilliams). In an Instagram post, Williams draws back the veil of an uncontested normality to ask that we take personal responsibility over the words we use. He writes: here’s a tip: Take the words DARK or BLACK in connection to bad, evil, ominous or scary events out of your vocabulary. We learn the stock market crashed on Black Monday, we read headlines that purport “Dark Days Ahead”. There’s “dark” or “black” humour which implies an undertone of evil, and then there are people like me who grow up with dark skin having to make sense of the English/American lexicon and its history of “fair complexions” – where “fair” can mean “light; blond.” OR “in accordance with rules or standards; legitimate.” We may not be fully responsible for the duplicitous evolution of language and subtle morphing of inherited beliefs into description yet we are in full command of the words we choose even as they reveal the questions we’ve left unasked. Like the work of Moreton-Robinson and other scholars, Williams implores his followers to take a reflexive position to consider the questions often left unasked. In doing so, he calls for the transcendence of anonymity and engagement with the realities of colonisation – no matter how ugly, confronting, and complicit one may be in its continuation. In the Australian context this means confronting how terms such as “dark”, “darkie”, or “darky” were historically used as derogatory and offensive slurs for Aboriginal peoples. Such language continues to be used today and can be found in the comment sections of social media, online news platforms, and other online forums (Carlson “Love and Hate”). Taking the move to execute personal accountability can be difficult. It can destabilise and reframe the ways in which we understand and interact with the world (Rose 22). For some, however, exposing racism and seemingly mundane aspects of society is taken as a personal attack which is often met with reactionary responses where one remains closed to new insights (Whittaker). This feeds into fears and anxieties pertaining to the perceived loss of power. These fears and anxieties continue to surface through conversations and calls for action on issues such as changing the date of Australia Day, the racialised reporting of news (McQuire), removing of plaques and statues known to be racist, and requests to change placenames and the names of products. For example, in 2020, Australian cheese producer Saputo Dairy Australia changed the name of it is popular brand “Coon” to “Cheer Tasty”. The decision followed a lengthy campaign led by Dr Stephen Hagan who called for the rebranding based on the Coon brand having racist connotations (ABC). The term has its racist origins in the United States and has long been used as a slur against people with dark skin, liking them to racoons and their tendency to steal and deceive. The term “Coon” is used in Australia by settlers as a racist term for referring to Aboriginal peoples. Claims that the name change is example of political correctness gone astray fail to acknowledge and empathise with the lived experience of being treated as if one is dirty, lazy, deceitful, or untrustworthy. Other brand names have also historically utilised racist wording along with imagery in their advertising (Conor). Pear’s soap for example is well-known for its historical use of racist words and imagery to legitimise white rule over Indigenous colonies, including in Australia (Jackson). Like most racial epithets, the power of language lies in how the words reflect and translate into actions that dehumanise others. The words we use matter. The everyday “ordinary” world, including online, is deeply politicised (Carlson and Frazer “They Got Filters”) and comes to reflect attitudes and power imbalances that encourage white people to internalise the falsity that they are superior and should have control over Black people (Conor). Decisions to make social change, such as that made by Saputo Dairy Australia, can manifest into further white anxieties via their ability to force the confrontation of the circ*mstances that continue to contribute to one’s own prosperity. In other words, to unveil the realities of colonialism and ask the questions that are too often left in the dark. Lived Experiences of Darkness Colonial anxieties and fears are driven by the fact that Black populations in many areas of the world are often characterised as criminals, perpetrators, threats, or nuisances, but are rarely seen as victims. In Australia, the repeated lack of police response and receptivity to concerns of Indigenous peoples expressed during the Black Lives Matter campaign saw tens of thousands of people take to the streets to protest. Protestors at the same time called for the end of police brutality towards Indigenous peoples and for an end to Indigenous deaths in custody. The protests were backed by a heavy online presence that sought to mobilise people in hope of lifting the veil that shrouds issues relating to systemic racism. There have been over 450 Aboriginal and Torres Strait Islander people to die in custody since the end of the Royal Commission into Aboriginal Deaths in Custody in 1991 (The Guardian). The tragedy of the Indigenous experience gains little attention internationally. The negative implications of being the object of white fear and anxiety are felt by Indigenous and other Black communities daily. The “safety signals” (Daniella Emanuel) adopted by white peoples in response to often irrational perceptions of threat signify how Indigenous and other Black peoples and communities are seen and valued by the hegemony. Memes played out in social media depicting “Karens” – a term that corresponds to caricaturised white women (but equally applicable to men) who exhibit behaviours of entitlement – have increasing been used in media to expose the prevalence of irrational racial fears (also see Wong). Police are commonly called on Indigenous people and other Black people for simply being within spaces such as shopping malls, street corners, parks, or other spaces in which they are considered not to belong (Mohdin). Digital media are also commonly envisioned as a space that is not natural or normal for Indigenous peoples, a notion that maintains narratives of so-called Indigenous primitivity (Carlson and Frazer). Media connotations of darkness as threatening are associated with, and strategically manipulated by, the images that accompany stories about Indigenous peoples and other Black peoples. Digital technologies play significant roles in producing and disseminating the images shown in the media. Moreover, they have a “role in mediating and amplifying old and new forms of abuse, hate, and discrimination” (Matamoros-Fernández and Farkas). Daniels demonstrates how social media sites can be spaces “where race and racism play out in interesting, sometimes disturbing, ways” (702), shaping ongoing colonial fears and anxieties over Black peoples. Prominent footballer Adam Goodes, for example, faced a string of attacks after he publicly condemned racism when he was called an “Ape” by a spectator during a game celebrating Indigenous contributions to the sport (Coram and Hallinan). This was followed by a barrage of personal attacks, criticisms, and booing that spread over the remaining years of his football career. When Goodes performed a traditional war dance as a form of celebration during a game in 2015, many turned to social media to express their outrage over his “confrontational” and “aggressive” behaviour (Robinson). Goodes’s affirmation of his Indigeneity was seen by many as a threat to their own positionality and white sensibility. Social media were therefore used as a mechanism to control settler narratives and maintain colonial power structures by framing the conversation through a white lens (Carlson and Frazer “They Got Filters”). Indigenous peoples in other highly visible fields have faced similar backlash. In 1993, Elaine George was the first Aboriginal person to feature on the cover of Vogue magazine, a decision considered “risky” at the time (Singer). The editor of Vogue later revealed that the cover was criticised by some who believed George’s skin tone was made to appear lighter than it actually was and that it had been digitally altered. The failure to accept a lighter skin colour as “Aboriginal” exposes a neglect to accept ethnicity and Blackness in all its diversity (Carlson and Frazer “They Got Filters”; Carlson “Love and Hate”). Where Adam Goodes was criticised for his overt expression of Blackness, George was critisised for not being “black enough”. It was not until seventeen years later that another Aboriginal model, Samantha Harris, was featured on the cover of Vogue (Marks). While George inspired and pathed the way for those to come, Harris experienced similar discrimination within the industry and amongst the public (Carson and Ky). Singer Jessica Mauboy (in Hornery) also explains how her identity was managed by others. She recalls, I was pretty young when I first received recognition, and for years I felt as though I couldn't show my true identity. What I was saying in public was very dictated by other people who could not handle my sense of culture and identity. They felt they had to take it off my hands. Mauboy’s experience not only demonstrates how Blackness continues to be seen as something to “handle”, but also how power imbalances play out. Scholar Chelsea Watego offers numerous examples of how this occurs in different ways and arenas, for example through relationships between people and within workplaces. Bargallie’s scholarly work also provides an understanding of how Indigenous people experience racism within the Australian public service, and how it is maintained through the structures and systems of power. The media often represents communities with large Indigenous populations as being separatist and not contributing to wider society and problematic (McQuire). Violence, and the threat of violence, is often presented in media as being normalised. Recently there have been calls for an increased police presence in Alice Springs, NT, and other remotes communities due to ongoing threats of “tribal payback” and acts of “lawlessness” (Sky News Australia; Hildebrand). Goldberg uses the phrase “Super/Vision” to describe the ways that Black men and women in Black neighbourhoods are continuously and erroneously supervised and surveilled by police using apparatus such as helicopters and floodlights. Simone Browne demonstrates how contemporary surveillance practices are rooted in anti-black domination and are operationalised through a white gaze. Browne uses the term “racializing surveillance” to describe a ”technology of social control where surveillance practices, policies, and performances concern the production of norms pertaining to race and exercise a ‘power to define what is in or out of place’” (16). The outcome is often discriminatory treatment to those negatively racialised by such surveillance. Narratives that associate Indigenous peoples with darkness and danger fuel colonial fears and uphold the invisible regimes of power by instilling the perception that acts of surveillance and the restrictions imposed on Indigenous peoples’ autonomy are not only necessary but justified. Such myths fail to contextualise the historic colonial factors that drive segregation and enable a forgetting that negates personal accountability and complicity in maintaining colonial power imbalances (Riggs and Augoustinos). Inayatullah and Blaney (165) write that the “myth we construct calls attention to a darker, tragic side of our ethical engagement: the role of colonialism in constituting us as modern actors.” They call for personal accountability whereby one confronts the notion that we are both products and producers of a modernity rooted in a colonialism that maintains the misguided notion of white supremacy (Wolfe; Mignolo; Moreton-Robinson). When Indigenous and other Black peoples enter spaces that white populations don’t traditionally associate as being “natural” or “fitting” for them (whether residential, social, educational, a workplace, online, or otherwise), alienation, discrimination, and criminalisation often occurs (Bargallie; Mohdin; Linhares). Structural barriers are erected, prohibiting career or social advancement while making the space feel unwelcoming (Fredericks; Bargallie). In workplaces, Indigenous employees become the subject of hyper-surveillance through the supervision process (Bargallie), continuing to make them difficult work environments. This is despite businesses and organisations seeking to increase their Indigenous staff numbers, expressing their need to change, and implementing cultural competency training (Fredericks and Bargallie). As Barnwell correctly highlights, confronting white fears and anxieties must be the responsibility of white peoples. When feelings of shock or discomfort arise when in the company of Indigenous peoples, one must reflexively engage with the reasons behind this “fear of the dark” and consider that perhaps it is they who are self-segregating. Mohdin suggests that spaces highly populated by Black peoples are best thought of not as “black spaces” or “black communities”, but rather spaces where white peoples do not want to be. They stand as reminders of a failed colonial regime that sought to deny and dehumanise Indigenous peoples and cultures, as well as the continuation of Black resistance and sovereignty. Conclusion In working towards improving relationships between Black and white populations, the truths of colonisation, and its continuing pervasiveness in local and global settings must first be confronted. In this article we have discussed the association of darkness with instinctual fears and negative responses to the unknown. White populations need to reflexively engage and critique how they think, act, present, address racism, and respond to Indigenous peoples (Bargallie; Moreton-Robinson; Whittaker), cultivating a “decolonising consciousness” (Bradfield) to develop new habits of thinking and relating. To overcome fears of the dark, we must confront that which remains unknown, and the questions left unasked. This means exposing racism and power imbalances, developing meaningful relationships with Indigenous peoples, addressing structural change, and implementing alternative ways of knowing and doing. Only then may we begin to embody Megan Cope’s message, “I’m not afraid of the Dark”. Acknowledgements We thank Dr Debbie Bargallie for her feedback on our article, which strengthened the work. References ABC News. 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Abstract:

Although I'm a rich white guy, I'm also a feminist anti-racism activist who fights for the rights of the poor and oppressed. (Carl Kenner)Systemic bias is a scourge to the pillar of neutrality. (Cerejota)Count me in. Let's leave the bias to the mainstream media. (Orcar967)Because this is so important. (CuttingEdge)These are a handful of comments posted by online editors who have banded together in a virtual coalition to combat Western bias on the world’s largest digital encyclopedia, Wikipedia. This collective action by Wikipedians both acknowledges the inherent inequalities of a user-controlled information project like Wikpedia and highlights the potential for progressive change within that same project. These community members are taking the responsibility of social change into their own hands (or more aptly, their own keyboards).In recent years much research has emerged on Wikipedia from varying fields, ranging from computer science, to business and information systems, to the social sciences. While critical at times of Wikipedia’s growth, governance, and influence, most of this work observes with optimism that barriers to improvement are not firmly structural, but rather they are socially constructed, leaving open the possibility of important and lasting change for the better.WikiProject: Countering Systemic Bias (WP:CSB) considers one such collective effort. Close to 350 editors have signed on to the project, which began in 2004 and itself emerged from a similar project named CROSSBOW, or the “Committee Regarding Overcoming Serious Systemic Bias on Wikipedia.” As a WikiProject, the term used for a loose group of editors who collaborate around a particular topic, these editors work within the Wikipedia site and collectively create a social network that is unified around one central aim—representing the un- and underrepresented—and yet they are bound by no particular unified set of interests. The first stage of a multi-method study, this paper looks at a snapshot of WP:CSB’s activity from both content analysis and social network perspectives to discover “who” geographically this coalition of the unrepresented is inserting into the digital annals of Wikipedia.Wikipedia and WikipediansDeveloped in 2001 by Internet entrepreneur Jimmy Wales and academic Larry Sanger, Wikipedia is an online collaborative encyclopedia hosting articles in nearly 250 languages (Cohen). The English-language Wikipedia contains over 3.2 million articles, each of which is created, edited, and updated solely by users (Wikipedia “Welcome”). At the time of this study, Alexa, a website tracking organisation, ranked Wikipedia as the 6th most accessed site on the Internet. Unlike the five sites ahead of it though—Google, Facebook, Yahoo, YouTube (owned by Google), and live.com (owned by Microsoft)—all of which are multibillion-dollar businesses that deal more with information aggregation than information production, Wikipedia is a non-profit that operates on less than $500,000 a year and staffs only a dozen paid employees (Lih). Wikipedia is financed and supported by the WikiMedia Foundation, a charitable umbrella organisation with an annual budget of $4.6 million, mainly funded by donations (Middleton).Wikipedia editors and contributors have the option of creating a user profile and participating via a username, or they may participate anonymously, with only an IP address representing their actions. Despite the option for total anonymity, many Wikipedians have chosen to visibly engage in this online community (Ayers, Matthews, and Yates; Bruns; Lih), and researchers across disciplines are studying the motivations of these new online collectives (Kane, Majchrzak, Johnson, and Chenisern; Oreg and Nov). The motivations of open source software contributors, such as UNIX programmers and programming groups, have been shown to be complex and tied to both extrinsic and intrinsic rewards, including online reputation, self-satisfaction and enjoyment, and obligation to a greater common good (Hertel, Niedner, and Herrmann; Osterloh and Rota). Investigation into why Wikipedians edit has indicated multiple motivations as well, with community engagement, task enjoyment, and information sharing among the most significant (Schroer and Hertel). Additionally, Wikipedians seem to be taking up the cause of generativity (a concern for the ongoing health and openness of the Internet’s infrastructures) that Jonathan Zittrain notably called for in The Future of the Internet and How to Stop It. Governance and ControlAlthough the technical infrastructure of Wikipedia is built to support and perhaps encourage an equal distribution of power on the site, Wikipedia is not a land of “anything goes.” The popular press has covered recent efforts by the site to reduce vandalism through a layer of editorial review (Cohen), a tightening of control cited as a possible reason for the recent dip in the number of active editors (Edwards). A number of regulations are already in place that prevent the open editing of certain articles and pages, such as the site’s disclaimers and pages that have suffered large amounts of vandalism. Editing wars can also cause temporary restrictions to editing, and Ayers, Matthews, and Yates point out that these wars can happen anywhere, even to Burt Reynold’s page.Academic studies have begun to explore the governance and control that has developed in the Wikipedia community, generally highlighting how order is maintained not through particular actors, but through established procedures and norms. Konieczny tested whether Wikipedia’s evolution can be defined by Michels’ Iron Law of Oligopoly, which predicts that the everyday operations of any organisation cannot be run by a mass of members, and ultimately control falls into the hands of the few. Through exploring a particular WikiProject on information validation, he concludes:There are few indicators of an oligarchy having power on Wikipedia, and few trends of a change in this situation. The high level of empowerment of individual Wikipedia editors with regard to policy making, the ease of communication, and the high dedication to ideals of contributors succeed in making Wikipedia an atypical organization, quite resilient to the Iron Law. (189)Butler, Joyce, and Pike support this assertion, though they emphasise that instead of oligarchy, control becomes encapsulated in a wide variety of structures, policies, and procedures that guide involvement with the site. A virtual “bureaucracy” emerges, but one that should not be viewed with the negative connotation often associated with the term.Other work considers control on Wikipedia through the framework of commons governance, where “peer production depends on individual action that is self-selected and decentralized rather than hierarchically assigned. Individuals make their own choices with regard to resources managed as a commons” (Viegas, Wattenberg and McKeon). The need for quality standards and quality control largely dictate this commons governance, though interviewing Wikipedians with various levels of responsibility revealed that policies and procedures are only as good as those who maintain them. Forte, Larco, and Bruckman argue “the Wikipedia community has remained healthy in large part due to the continued presence of ‘old-timers’ who carry a set of social norms and organizational ideals with them into every WikiProject, committee, and local process in which they take part” (71). Thus governance on Wikipedia is a strong representation of a democratic ideal, where actors and policies are closely tied in their evolution. Transparency, Content, and BiasThe issue of transparency has proved to be a double-edged sword for Wikipedia and Wikipedians. The goal of a collective body of knowledge created by all—the “expert” and the “amateur”—can only be upheld if equal access to page creation and development is allotted to everyone, including those who prefer anonymity. And yet this very option for anonymity, or even worse, false identities, has been a sore subject for some in the Wikipedia community as well as a source of concern for some scholars (Santana and Wood). The case of a 24-year old college dropout who represented himself as a multiple Ph.D.-holding theology scholar and edited over 16,000 articles brought these issues into the public spotlight in 2007 (Doran; Elsworth). Wikipedia itself has set up standards for content that include expectations of a neutral point of view, verifiability of information, and the publishing of no original research, but Santana and Wood argue that self-policing of these policies is not adequate:The principle of managerial discretion requires that every actor act from a sense of duty to exercise moral autonomy and choice in responsible ways. When Wikipedia’s editors and administrators remain anonymous, this criterion is simply not met. It is assumed that everyone is behaving responsibly within the Wikipedia system, but there are no monitoring or control mechanisms to make sure that this is so, and there is ample evidence that it is not so. (141) At the theoretical level, some downplay these concerns of transparency and autonomy as logistical issues in lieu of the potential for information systems to support rational discourse and emancipatory forms of communication (Hansen, Berente, and Lyytinen), but others worry that the questionable “realities” created on Wikipedia will become truths once circulated to all areas of the Web (Langlois and Elmer). With the number of articles on the English-language version of Wikipedia reaching well into the millions, the task of mapping and assessing content has become a tremendous endeavour, one mostly taken on by information systems experts. Kittur, Chi, and Suh have used Wikipedia’s existing hierarchical categorisation structure to map change in the site’s content over the past few years. Their work revealed that in early 2008 “Culture and the arts” was the most dominant category of content on Wikipedia, representing nearly 30% of total content. People (15%) and geographical locations (14%) represent the next largest categories, while the natural and physical sciences showed the greatest increase in volume between 2006 and 2008 (+213%D, with “Culture and the arts” close behind at +210%D). This data may indicate that contributing to Wikipedia, and thus spreading knowledge, is growing amongst the academic community while maintaining its importance to the greater popular culture-minded community. Further work by Kittur and Kraut has explored the collaborative process of content creation, finding that too many editors on a particular page can reduce the quality of content, even when a project is well coordinated.Bias in Wikipedia content is a generally acknowledged and somewhat conflicted subject (Giles; Johnson; McHenry). The Wikipedia community has created numerous articles and pages within the site to define and discuss the problem. Citing a survey conducted by the University of Würzburg, Germany, the “Wikipedia:Systemic bias” page describes the average Wikipedian as:MaleTechnically inclinedFormally educatedAn English speakerWhiteAged 15-49From a majority Christian countryFrom a developed nationFrom the Northern HemisphereLikely a white-collar worker or studentBias in content is thought to be perpetuated by this demographic of contributor, and the “founder effect,” a concept from genetics, linking the original contributors to this same demographic has been used to explain the origins of certain biases. Wikipedia’s “About” page discusses the issue as well, in the context of the open platform’s strengths and weaknesses:in practice editing will be performed by a certain demographic (younger rather than older, male rather than female, rich enough to afford a computer rather than poor, etc.) and may, therefore, show some bias. Some topics may not be covered well, while others may be covered in great depth. No educated arguments against this inherent bias have been advanced.Royal and Kapila’s study of Wikipedia content tested some of these assertions, finding identifiable bias in both their purposive and random sampling. They conclude that bias favoring larger countries is positively correlated with the size of the country’s Internet population, and corporations with larger revenues work in much the same way, garnering more coverage on the site. The researchers remind us that Wikipedia is “more a socially produced document than a value-free information source” (Royal & Kapila).WikiProject: Countering Systemic BiasAs a coalition of current Wikipedia editors, the WikiProject: Countering Systemic Bias (WP:CSB) attempts to counter trends in content production and points of view deemed harmful to the democratic ideals of a valueless, open online encyclopedia. WP:CBS’s mission is not one of policing the site, but rather deepening it:Generally, this project concentrates upon remedying omissions (entire topics, or particular sub-topics in extant articles) rather than on either (1) protesting inappropriate inclusions, or (2) trying to remedy issues of how material is presented. Thus, the first question is "What haven't we covered yet?", rather than "how should we change the existing coverage?" (Wikipedia, “Countering”)The project lays out a number of content areas lacking adequate representation, geographically highlighting the dearth in coverage of Africa, Latin America, Asia, and parts of Eastern Europe. WP:CSB also includes a “members” page that editors can sign to show their support, along with space to voice their opinions on the problem of bias on Wikipedia (the quotations at the beginning of this paper are taken from this “members” page). At the time of this study, 329 editors had self-selected and self-identified as members of WP:CSB, and this group constitutes the population sample for the current study. To explore the extent to which WP:CSB addressed these self-identified areas for improvement, each editor’s last 50 edits were coded for their primary geographical country of interest, as well as the conceptual category of the page itself (“P” for person/people, “L” for location, “I” for idea/concept, “T” for object/thing, or “NA” for indeterminate). For example, edits to the Wikipedia page for a single person like Tony Abbott (Australian federal opposition leader) were coded “Australia, P”, while an edit for a group of people like the Manchester United football team would be coded “England, P”. Coding was based on information obtained from the header paragraphs of each article’s Wikipedia page. After coding was completed, corresponding information on each country’s associated continent was added to the dataset, based on the United Nations Statistics Division listing.A total of 15,616 edits were coded for the study. Nearly 32% (n = 4962) of these edits were on articles for persons or people (see Table 1 for complete coding results). From within this sub-sample of edits, a majority of the people (68.67%) represented are associated with North America and Europe (Figure A). If we break these statistics down further, nearly half of WP:CSB’s edits concerning people were associated with the United States (36.11%) and England (10.16%), with India (3.65%) and Australia (3.35%) following at a distance. These figures make sense for the English-language Wikipedia; over 95% of the population in the three Westernised countries speak English, and while India is still often regarded as a developing nation, its colonial British roots and the emergence of a market economy with large, technology-driven cities are logical explanations for its representation here (and some estimates make India the largest English-speaking nation by population on the globe today).Table A Coding Results Total Edits 15616 (I) Ideas 2881 18.45% (L) Location 2240 14.34% NA 333 2.13% (T) Thing 5200 33.30% (P) People 4962 31.78% People by Continent Africa 315 6.35% Asia 827 16.67% Australia 175 3.53% Europe 1411 28.44% NA 110 2.22% North America 1996 40.23% South America 128 2.58% The areas of the globe of main concern to WP:CSB proved to be much less represented by the coalition itself. Asia, far and away the most populous continent with more than 60% of the globe’s people (GeoHive), was represented in only 16.67% of edits. Africa (6.35%) and South America (2.58%) were equally underrepresented compared to both their real-world populations (15% and 9% of the globe’s population respectively) and the aforementioned dominance of the advanced Westernised areas. However, while these percentages may seem low, in aggregate they do meet the quota set on the WP:CSB Project Page calling for one out of every twenty edits to be “a subject that is systematically biased against the pages of your natural interests.” By this standard, the coalition is indeed making headway in adding content that strategically counterbalances the natural biases of Wikipedia’s average editor.Figure ASocial network analysis allows us to visualise multifaceted data in order to identify relationships between actors and content (Vego-Redondo; Watts). Similar to Davis’s well-known sociological study of Southern American socialites in the 1930s (Scott), our Wikipedia coalition can be conceptualised as individual actors united by common interests, and a network of relations can be constructed with software such as UCINET. A mapping algorithm that considers both the relationship between all sets of actors and each actor to the overall collective structure produces an image of our network. This initial network is bimodal, as both our Wikipedia editors and their edits (again, coded for country of interest) are displayed as nodes (Figure B). Edge-lines between nodes represents a relationship, and here that relationship is the act of editing a Wikipedia article. We see from our network that the “U.S.” and “England” hold central positions in the network, with a mass of editors crowding around them. A perimeter of nations is then held in place by their ties to editors through the U.S. and England, with a second layer of editors and poorly represented nations (Gabon, Laos, Uzbekistan, etc.) around the boundaries of the network.Figure BWe are reminded from this visualisation both of the centrality of the two Western powers even among WP:CSB editoss, and of the peripheral nature of most other nations in the world. But we also learn which editors in the project are contributing most to underrepresented areas, and which are less “tied” to the Western core. Here we see “Wizzy” and “Warofdreams” among the second layer of editors who act as a bridge between the core and the periphery; these are editors with interests in both the Western and marginalised nations. Located along the outer edge, “Gallador” and “Gerrit” have no direct ties to the U.S. or England, concentrating all of their edits on less represented areas of the globe. Identifying editors at these key positions in the network will help with future research, informing interview questions that will investigate their interests further, but more significantly, probing motives for participation and action within the coalition.Additionally, we can break the network down further to discover editors who appear to have similar interests in underrepresented areas. Figure C strips down the network to only editors and edits dealing with Africa and South America, the least represented continents. From this we can easily find three types of editors again: those who have singular interests in particular nations (the outermost layer of editors), those who have interests in a particular region (the second layer moving inward), and those who have interests in both of these underrepresented regions (the center layer in the figure). This last group of editors may prove to be the most crucial to understand, as they are carrying the full load of WP:CSB’s mission.Figure CThe End of Geography, or the Reclamation?In The Internet Galaxy, Manuel Castells writes that “the Internet Age has been hailed as the end of geography,” a bold suggestion, but one that has gained traction over the last 15 years as the excitement for the possibilities offered by information communication technologies has often overshadowed structural barriers to participation like the Digital Divide (207). Castells goes on to amend the “end of geography” thesis by showing how global information flows and regional Internet access rates, while creating a new “map” of the world in many ways, is still closely tied to power structures in the analog world. The Internet Age: “redefines distance but does not cancel geography” (207). The work of WikiProject: Countering Systemic Bias emphasises the importance of place and representation in the information environment that continues to be constructed in the online world. This study looked at only a small portion of this coalition’s efforts (~16,000 edits)—a snapshot of their labor frozen in time—which itself is only a minute portion of the information being dispatched through Wikipedia on a daily basis (~125,000 edits). Further analysis of WP:CSB’s work over time, as well as qualitative research into the identities, interests and motivations of this collective, is needed to understand more fully how information bias is understood and challenged in the Internet galaxy. The data here indicates this is a fight worth fighting for at least a growing few.ReferencesAlexa. “Top Sites.” Alexa.com, n.d. 10 Mar. 2010 ‹http://www.alexa.com/topsites>. Ayers, Phoebe, Charles Matthews, and Ben Yates. How Wikipedia Works: And How You Can Be a Part of It. 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